AP3B2 Antibody - BSA Free

Western Blot: AP3B2 Antibody - BSA Free [NBP1-76590] - Western blot analysis of AP3B2 expression in mouse brain tissue lysate with AP3B2 antibody at (A) 1 and (B) 2 u/ml.

Immunohistochemistry: AP3B2 Antibody - BSA Free [NBP1-76590] - Immunohistochemistry of AP3B2 in mouse brain tissue with AP3B2 antibody at 5 ug/mL.

Immunocytochemistry/ Immunofluorescence: AP3B2 Antibody - BSA Free [NBP1-76590] - Immunofluorescence of AP3B2 in mouse brain tissue with AP3B2 antibody at 20 ug/mL.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

AP3B2 Antibody - BSA Free Summary

• Immunogen: Antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human AP3B2. The immunogen is located within the first 50 amino acids of AP3B2. Amino Acid Squence: IFSSDYKRHDDLKEMLDT
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: AP3B2
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 20 ug/ml
  • Immunohistochemistry 5 ug/ml
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Western Blot 1-2 ug/ml
• Theoretical MW: 128 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control Peptide:

  AP3B2 Peptide (NBP1-76590PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for AP3B2 Antibody - BSA Free

• Adapter-related protein complex 3 subunit beta-2
• Adaptor protein complex AP-3 subunit beta-2
• adaptor-related protein complex 3, beta 2 subunit
• beta-3B-adaptin
• Clathrin assembly protein complex 3 beta-2 large chain
• DKFZp686D17136
• NAPTBAP-3 complex subunit beta-2
• Neuronal adaptin-like protein, beta-subunit
• Neuron-specific vesicle coat protein beta-NAP

Background

The AP3 complex is a heterotetramer composed of two large adaptins (AP3D1, AP3B1 or AP3B2), a medium adaptin (AP3M1 or AP3M2) and a small adaptin (APS1 or AP3S2). The complex is associated with the Golgi region as well as more peripheral structures. It facilitates the budding of vesicles from the Golgi membrane and may be directly involved in trafficking to lysosomes. Unlike the homologous AP3B1, AP3B2-containing AP3 complexes are preferentially targeted to neuronal processes, and mice deficient in AP3B2 displayed compromised synaptic zinc stores.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: AP3B2