AP2A2 Antibody - BSA Free

Western Blot: AP2A2 Antibody [NBP3-12692] - Lane A: RT-4 Whole Cell LysateLane B: U-251 MG Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:104 kDaObserved band size:104 kDa

Immunohistochemistry-Paraffin: AP2A2 Antibody [NBP3-12692] - Staining of human AP2A2 in human rectum with rabbit polyclonal antibody at 1:100 dilution.

Immunohistochemistry-Paraffin: AP2A2 Antibody [NBP3-12692] - Staining of human AP2A2 in human testis with rabbit polyclonal antibody at 1:100 dilution.

Immunoprecipitation: AP2A2 Antibody [NBP3-12692] - Lane A:0.5 mg RT-4 Whole Cell Lysate4 uL anti-AP2A2 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-AP2A2 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 104 kDaObserved band size :104 kDa

• Reactivity: Hu
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

AP2A2 Antibody - BSA Free Summary

• Description: This antibody can be stored at 2-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
  
  Protein A & Antigen Affinity purified
• Immunogen: Produced in rabbits immunized with E. coli-derived Human AP2A2 fragment
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: AP2A2
• Purity: Antigen and protein A Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation 1-5uL/mg of uLysate
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.03% Proclin 300
• Purity: Antigen and protein A Affinity-purified

Alternate Names for AP2A2 Antibody - BSA Free

• 100 kDa coated vesicle protein C
• Adapter-related protein complex 2 alpha-2 subunit
• adaptin, alpha B
• Adaptor protein complex AP-2 subunit alpha-2
• adaptor-related protein complex 2, alpha 2 subunit
• alpha2-adaptin
• Alpha-adaptin C
• alpha-adaptin C; Huntingtin interacting protein J
• AP-2 complex subunit alpha-2
• Clathrin assembly protein complex 2 alpha-C large chain
• clathrin-associated/assembly/adaptor protein, large, alpha 2
• DKFZP564D1864
• HIP9ADTAB
• Huntingtin yeast partner J
• Huntingtin-interacting protein 9
• Huntingtin-interacting protein J
• HYPJCLAPA2
• KIAA0899HIP-9
• Plasma membrane adaptor HA2/AP2 adaptin alpha C subunit

Background

AP2A2 is a component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transportvia transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat componentsand appear to be involved in carg

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: AP2A2