AP1S2 Recombinant Protein Antigen

• Reactivity: Hu
• Applications: AC

AP1S2 Recombinant Protein Antigen Summary

• Description: A recombinant protein antigen with a N-terminal His6-ABP tag corresponding to human AP1S2.
  
  

  Source: E. coli

  Amino Acid Sequence: QADLLQEKTETMYHSKSFIGFKKAY

  Fusion Tag: N-terminal His6ABP (ABP = Albumin Binding Protein derived from Streptococcal Protein G)

  This product is intended to be used as a blocking antigen for antibody competition assays. Any other use of this antigen is done at the risk of the user. The use of this product for commercial production is strictly prohibited. Please contact technical support if you have any questions.

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein Antigen
• Gene: AP1S2
• Purity: >80% by SDS-PAGE and Coomassie blue staining

Applications/Dilutions

• Dilutions:
  • Antibody Competition 10 - 100 molar excess
• Application Notes: This recombinant antigen is only intended to be used as a blocking agent to confirm antibody specificity with the corresponding antibody, catalog number NBP2-14299.

  It is purified by IMAC chromatography, and the expected concentration is greater than 0.5 mg/ml.

  For current lot information, including availability, please contact our technical support team click nb-technical@bio-techne.com

• Theoretical MW: 21 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS and 1M Urea, pH 7.4.
• Preservative: No Preservative
• Purity: >80% by SDS-PAGE and Coomassie blue staining

Alternate Names for AP1S2 Recombinant Protein Antigen

• adaptor-related protein complex 1, sigma 2 subunit
• MGC:1902
• SIGMA1B
• X-linked 59

Background

Adaptor protein complex 1 is found at the cytoplasmic face of coated vesicles located at the Golgi complex, where itmediates both the recruitment of clathrin to the membrane and the recognition of sorting signals within the cytosolictails of transmembrane receptors. This complex is a heterotetramer composed of two large, one medium, and one smalladaptin subunit. The protein encoded by this gene serves as the small subunit of this complex and is a member of theadaptin protein family. Transcript variants utilizing alternative polyadenylation signals exist for this gene.(provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: AP1S2