ANKS3 Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: ANKS3 Antibody [NBP1-83531] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.

Staining of human Lung shows moderate cytoplasmic positivity in pneumocytes.

Staining of human Liver shows strong cytoplasmic positivity in hepatocytes.

Staining of human Kidney shows moderate cytoplasmic positivity in cells in tubules.

Analysis in human cell line HEK 293.

Staining of human Heart muscle shows moderate cytoplasmic positivity in cardiomyocytes.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

ANKS3 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit ANKS3 Antibody - BSA Free (NBP1-83531) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: REEHAFCANLGPVQSSSSSEGLARAQGLSSEASVESNEDSDHACKSSARKQAKSYMKTKNPDSQWPPRTATDREGFLAESSPQTQRAPYSGPQD
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ANKS3
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  ANKS3 Protein (NBP1-83531PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for ANKS3 Antibody - BSA Free

• ankyrin repeat and SAM domain-containing protein 3
• ankyrin repeat and sterile alpha motif domain containing 3
• FLJ32767
• KIAA1977FLJ32345

Background

ANKS3 (ankyrin repeat and SAM domain-containing protein 3) contains 6 ANK (ankyrin) repeats and a SAM (sterile alpha motif) domain. Each ANK repeat consists of a 30-34 amino acid sequence that exhibits a helix-turn-helix conformation and functions to mediate protein-protein interactions. The SAM domain is a 70 amino acid domain that also functions to mediate protein-protein interactions for homo- and hetero- oligomerization. The function of ANKS3 has not been characterized.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ANKS3