AMPD1 Antibody

Western Blot: AMPD1 Antibody [NBP2-24508] - Analysis of AMPDA1 in rat skeletal muscle lysate in the 1) absence, 2) presence of immunizing peptide and 3) mouse skeletal muscle using this antibody. 5 ug/ml.

Immunohistochemistry-Paraffin: AMPD1 Antibody [NBP2-24508] - Staining of AMPD1 using this antibody at 5 ug/ml (using control rabbit Ig for figure B).

Immunohistochemistry-Paraffin: AMPD1 Antibody [NBP2-24508] - IHC analysis of human skeletal muscle using NBP2-24508 at 10 ug/ml.

Product Discontinued

AMPD1 Antibody Summary

• Immunogen: A portion of amino acids 500-550 of human AMPDA1 was used as the immunogen.
• Predicted Species: Chimpanzee (100%), Bovine (100%), Equine (100%), Monkey (100%), Chicken (93%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: AMPD1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 5-10 ug/ml
  • Western Blot 0.5-2 ug/ml

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: zebrafish (86%). The approximate 70 kD seen in western blot is blocked by the immunizing peptide.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.2 ml PBS and 0.2% Gelatin
• Preservative: 0.05% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for AMPD1 Antibody

• adenosine monophosphate deaminase 1 (isoform M)
• adenosine monophosphate deaminase 1
• adenosine monophosphate deaminase-1 (muscle)
• AMP deaminase 1
• AMP deaminase isoform M
• AMPD
• EC 3.5.4.6
• MAD
• MADA
• Myoadenylate deaminase
• skeletal muscle AMPD

Background

Adenosine monophosphate deaminase 1 catalyzes the deamination of AMP to IMP in skeletal muscle and plays an important role in the purine nucleotide cycle. Two other genes have been identified, AMPD2 and AMPD3, for the liver- and erythocyte-specific isoforms, respectively. Deficiency of the muscle-specific enzyme is apparently a common cause of exercise-induced myopathy and probably the most common cause of metabolic myopathy in the human.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for AMPD1 Antibody (NBP2-24508)(1)

Publication using NBP2-24508

• Huang Z, Zhang J, Gu Y et al. Analysis of the molecular mechanism of inosine monophosphate deposition in Jingyuan chicken muscles using a proteomic approach Poultry Science 2022-01-01 [PMID: 35259688]

Bioinformatics

• Gene Symbol: AMPD1