Recombinant Mouse AlphaB Crystallin/CRYAB Protein

SDS-Page: Recombinant Mouse AlphaB Crystallin/CRYAB Protein [NBC1-18376] - Crystallin alpha B, 20kDa (175aa), confirmed by MALDI-TOF with a purity of 95% by SDS - PAGE

• Reactivity: Mu
• Applications: PAGE
• Concentration: 1 mg/ml

Recombinant Mouse AlphaB Crystallin/CRYAB Protein Summary

• Description: An un-tagged recombinant protein corresponding to the amino acids 1-175 of Mouse AlphaB Crystallin/CRYAB
  
  

  Source: E.coli

  Amino Acid Sequence: MDIAIHHPWI RRPFFPFHSP SRLFDQFFGE HLLESDLFST ATSLSPFYLR PPSFLRAPSW IDTGLSEMRL EKDRFSVNLD VKHFSPEELK VKVLGDVIEV HGKHEERQDE HGFISREFHR KYRIPADVDP LTITSSLSSD GVLTVNGPRK QVSGPERTIP ITREEKPAVA AAPKK

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: CRYAB
• Purity: >95%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 20 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH 8.0), 10 % glycerol
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: >95%, by SDS-PAGE

Alternate Names for Recombinant Mouse AlphaB Crystallin/CRYAB Protein

• alpha B crystallin
• alpha(B)-crystallin
• AlphaB Crystallin
• alpha-crystallin B chain
• CRYA2
• CRYA2alpha crystallin B chain
• CRYAB
• crystallin, alpha B
• CTPP2
• Heat shock protein beta-5
• heat-shock 20 kD like-protein
• HSPB5
• Renal carcinoma antigen NY-REN-27
• Rosenthal fiber component

Background

Alpha crystallins are composed of two gene products, alpha-A and alpha-B for acidic and basic, respectively. Alpha crystallins can be induced by heat shock and are members of the small heat shock protein (sHSP also known as the HSP20). They act as molecular chaperones and hold them in large soluble aggregates. These heterogeneous aggregates consist of 30-40 subunits; the alpha-A and alpha-B subunits have a 3:1 ratio, respectively. Two additional functions of alpha-crystallins are an autokinase activity and participation in the intracellular architecture. CRYAB is expressed widely in many tissues and organs. Recombinant CRYAB was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: CRYAB