AlphaB Crystallin/CRYAB Antibody (CRYAB/4663) - Azide and BSA Free

Analysis of Protein Array containing more than 19,000 full-length human proteins using AlphaB Crystallin/CRYAB Mouse Monoclonal Antibody (CRYAB/4663).

Formalin-fixed, paraffin-embedded human heart stained with AlphaB Crystallin/CRYAB antibody (CRYAB/4663). Inset: PBS instead of primary antibody; secondary only negative control.

Formalin-fixed, paraffin-embedded human prostate cancer stained with AlphaB Crystallin/CRYAB antibody (CRYAB/4663). HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.

• Reactivity: Hu, Rt
• Applications: IHC
• Clone: CRYAB/4663
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

AlphaB Crystallin/CRYAB Antibody (CRYAB/4663) - Azide and BSA Free Summary

• Description: Positive Controls: 293T whole cell lysates; heart and brain.
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Non-hazardous. No MSDS required.
• Immunogen: Recombinant human full-length AlphaB Crystallin/CRYAB protein
• Localization: Cytoplasm, translocates to nucleus during heat shock and resides in nuclear splicing speckles.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: CRYAB
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry-Paraffin 1-2 ug/ml
• Application Notes: Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes. at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
  
  Optimal dilution for a specific application should be determined.

Reactivity Notes

Others-not tested

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C. Avoid freeze-thaw cycles.
• Buffer: 10mM PBS
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: Protein A or G purified

Alternate Names for AlphaB Crystallin/CRYAB Antibody (CRYAB/4663) - Azide and BSA Free

• alpha B crystallin
• alpha(B)-crystallin
• AlphaB Crystallin
• alpha-crystallin B chain
• CRYA2
• CRYA2alpha crystallin B chain
• CRYAB
• crystallin, alpha B
• CTPP2
• Heat shock protein beta-5
• heat-shock 20 kD like-protein
• HSPB5
• Renal carcinoma antigen NY-REN-27
• Rosenthal fiber component

Background

Crystallin AB is a member of the small heat shock protein family and expressed in several tissues including brain, kidney cardiac and skeletal muscle. Crystallin AB functions as a molecular chaperone and in intracellular architecture. Crystallin AB has shown to have interactions with CRYAA, CRYGC, CRYBB2, CRYBA1 and HSPB8. Defects in crystalline AB have been linked to cause myopathy myofibrillar type 2, cataract posterior polar type 2, and myopathy myofibrillar fatal infantile hypertonic alpha-B crystallin-related (MFMFIH-CRYAB).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CRYAB