alpha-1B Adrenergic R/ADRA1B Antibody (471802) [Unconjugated]

PC‑3 human prostate cancer cell line was stained with Mouse Anti-Human ADRA1B Monoclonal Antibody (Catalog # MAB4730, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2 Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

• Reactivity: Hu
• Applications: CyTOF-ready, Flow
• Clone: 471802
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

alpha-1B Adrenergic R/ADRA1B Antibody (471802) [Unconjugated] Summary

• Immunogen: NS0 mouse myeloma cell line transfected with human ADRA1B
  Met1-Phe520
  Accession # P35368
• Specificity: Detects human ADRA1B. Stains human ADRA1B transfectants but not irrelevant transfectants.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: ADRA1B
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Intracellular Staining by Flow Cytometry 0.25 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for alpha-1B Adrenergic R/ADRA1B Antibody (471802) [Unconjugated]

• a-1B AdrenergicR
• ADRA1
• ADRA1B
• adrenergic, alpha-1B-, receptor
• alpha-1B Adrenergic R
• alpha-1B adrenergic receptor
• alpha1B AdrenergicR
• Alpha-1B adrenoceptor
• Alpha-1B adrenoreceptor
• alpha-1B-adrenergic receptor
• ALPHA1BAR

Background

ADRA1B, also known as the alpha -1B-adrenergic receptor, is a 90 kDa 7TM catecholamine receptor with selectivity for epinephrine and norepinephrine. Formation of homooligomers and heterooligomers with ADRA1A enables receptor internalization following ligand binding. ADRA1B functions in diverse settings including vasoconstriction and myocardial contractility, neuronal dopaminergic responses, dendritic cell migration and inflammatory responses, and neuroendocrine regulation of fertility. Human ADRA1B shares 95% amino acid sequence identity with mouse and rat ADRA1B.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ADRA1B
• Uniprot:
  • P35368()