alpha-1A Adrenergic R/ADRA1A Antibody

Images

 
Western Blot: alpha-1A Adrenergic R/ADRA1A Antibody [NB100-78585] - Sample(30 ug of whole cell lysate)A:Raji (GTX27908)10% SDS PAGE, antibody diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: alpha-1A Adrenergic R/ADRA1A Antibody [NB100-78585] - analysis of methanol-fixed Hep3B, using alpha 1a Adrenergic Receptor antibody at 1:500 dilution.
Immunohistochemistry-Paraffin: alpha-1A Adrenergic R/ADRA1A Antibody [NB100-78585] - Paraffin-embedded Heart, using antibody 10 ug/ml.
Western Blot: alpha-1A Adrenergic R/ADRA1A Antibody [NB100-78585] - A. Jurkat whole cell lysate/extract. B. Raji whole cell lysate/extract. C. NCI-H929 whole cell lysate/extract. 10 % SDS-PAGE alpha 1a Adrenergic ...read more
Immunohistochemistry-Paraffin: alpha-1A Adrenergic R/ADRA1A Antibody [NB100-78585] - Paraffin-embedded mouse prostate. alpha 1a Adrenergic Receptor antibody dilution: 1:500.
Immunohistochemistry-Paraffin: alpha-1A Adrenergic R/ADRA1A Antibody [NB100-78585] - Paraffin-embedded rat hind brain. alpha 1a Adrenergic Receptor antibody dilution: 1:500.

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, Ca, GP, Mk, Rb, RMSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

alpha-1A Adrenergic R/ADRA1A Antibody Summary

Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human alpha 1a Adrenergic Receptor. The exact sequence is proprietary.
Localization
Cell membrane
Predicted Species
Porcine (100%), Rhesus Macaque (100%), Rabbit (100%), Bovine (100%), Monkey (100%), Guinea Pig (100%), Canine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ADRA1A
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
51 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.0), 1.0% BSA and 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for alpha-1A Adrenergic R/ADRA1A Antibody

  • a-1A AdrenergicR
  • ADRA1A
  • ADRA1Cadrenergic, alpha-1A-, receptor variant 1
  • ADRA1L1
  • adrenergic, alpha -1A-, receptor
  • adrenergic, alpha-1A-, receptor variant 3
  • adrenergic, alpha-1A-, receptor variant 5
  • adrenergic, alpha-1A-, receptor variant 8
  • adrenergic, alpha-1A-, receptor
  • adrenergic, alpha-1C-, receptor
  • alpha-1A Adrenergic R
  • alpha-1A adrenergic receptor
  • alpha1A AdrenergicR
  • Alpha-1A adrenoceptor
  • Alpha-1A adrenoreceptor
  • ALPHA1AAR
  • Alpha-1C adrenergic receptor
  • Alpha-adrenergic receptor 1c
  • G protein coupled receptor

Background

Adrenergic Receptors are members of the 7-transmembrane domain G protein-coupled receptor superfamily that bind the endogenous catecholamines epinephrine and norepinephrine. Pharmacological, structural and molecular cloning data indicate significant heterogeneity within this receptor family. Nine receptor subtypes have been identified to date, including three alpha 1 adrenergic receptor subtypes (1A, 1B and 1C), three alpha 2 adrenergic receptors (2A, 2B and 2C), and three beta adrenergic receptor subtypes (1, 2 and 3). Adrenergic receptors participate in either the onset or maintenance of several disease states including hypertension, cardiac dysfunction (congestive heart failure, ischemia, arrhythmias), diabetes, glaucoma, depression and impotence. Alpha 1 adrenergic receptor subtypes are found in numerous tissues and are involved in the regulation of blood pressure due to changes in vascular tone and cardiac output. Effects on uterine contraction, hepatic glucose metabolism, heat shock protein 70 (HSP 70), proto-oncogene expression, and mitogenesis have been linked to alpha 1 adrenergic receptor activation. Norepinephrine can increase nitric oxide synthase (NOS) levels in the hypothalamus by activating alpha 1 adrenergic receptor, which may indirectly suppress the release of luteinizing hormone (LH), the androgens, estrogen and progesterone.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for alpha-1A Adrenergic R/ADRA1A Antibody (NB100-78585). (Showing 1 - 1 of 1 FAQ).

  1. On the product sheet of NB100-78585, it states that Synthetic peptide contain a sequence corresponding to a region within amino acids 206 and 260 of alpha 1a Adrenergic Receptor. Is this sequence conserved for only alpha 1a Adrenergic receptor or also for the 2 other alpha 1 adrenergic receptor subtypes? It also states that the Raji cell lysates were used as the positive control. Is it an cell line with over expressed adrenergic receptor? Or it is only after transfection? Does your company provide any other form of positive controls for the alpha 1 adrenergic receptor detection?
    • The immunogen used to generate NB100-78585 is conserved for all 9 isoforms of ADRA1A and this antibody does not cross-react with other alpha 1 adrenergic receptor subtypes. The Raji cells used were not transfected. This blot shows endogenous expression. HL-60 cells can also be used as a positive control.

Secondary Antibodies

 

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Bioinformatics

Gene Symbol ADRA1A
Entrez
Uniprot