Allergin-1 Antibody (767727)

Images

 
Human peripheral blood monocytes were stained with Mouse Anti-Human CD14 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB3832F) and either (A) Mouse Anti-Human Allergin‑1 Monoclonal Antibody (Catalog # ...read more
Allergin‑1 was detected in immersion fixed U‑87 MG human glioblastoma/astrocytoma cell line using Mouse Anti-Human Allergin‑1 Monoclonal Antibody (Catalog # MAB7638) at 10 µg/mL for 3 hours at room temperature. ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, CyTOF-ready, ICC/IF
Clone
767727
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

Allergin-1 Antibody (767727) Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human Allergin-1
Met1-Lys227
Accession # Q7Z6M3
Specificity
Detects human Allergin-1 in direct ELISAs.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
MILR1
Purity
Protein A or G purified from hybridoma culture supernatant
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunocytochemistry 8-25 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Protein A or G purified from hybridoma culture supernatant
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Allergin-1 Antibody (767727)

  • Allergin1
  • Allergin-1
  • Allergy Inhibitory Receptor 1
  • C17orf60
  • Chromosome 17 Open Reading Frame 60
  • Mast Cell Antigen 32
  • Mast Cell Immunoglobulin-Like Receptor 1
  • MCA32
  • MCA-32
  • MCA32-1S2
  • MILR1
  • Probable Mast Cell Antigen 32 Homolog

Background

MCA32 (Mast Cell Antigen; also Allergin‑1 and c17orf60) is a 70 kDa member of the Ig Superfamily. It is expressed on mast cells, basophils, neutrophils and macrophages, and is reported to negatively regulate allergic mediator release from mast cells. In particular, MCA32 interacts with Fc epsilon RI. Upon IgE mediated Fc epsilon RI aggregation and activation, Allergin‑1 recruits Tyr phosphatases to a Fc epsilon RI tetrameric complex, dampening IgE receptor downstream signaling. Mature human Allergin‑1 is a monomeric type I transmembrane glycoprotein that is 324 amino acids (aa) in length. It contains two C2-type Ig domains (aa 35-118 and 123-217) in the extracellular region (aa 20-227), plus a 95 aa cytoplasmic domain that possesses two ITIM motifs. Two 40 kDa splice variants exist that show deletions of the Ig-like domains. One isoform termed Allergin‑1S1 is missing aa 128-217, while a second isoform called Allergin‑1S2 is missing aa 33-122. This second isoform represents the antigen used for immunization in this product (GenBank BAJ08253). Over aa 29-131, the Allergin‑1S2 isoform shares 100% sequence identity with human Allergin‑1L full length isoform and only 24% aa sequence identity with mouse Allergin‑1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 FAB7638U
Alexa Fluor 405 FAB7638V
Alexa Fluor 488 FAB7638G
Alexa Fluor 532 FAB7638X
Alexa Fluor 594 FAB7638T
Alexa Fluor 647 FAB7638R
Alexa Fluor 700 FAB7638N
Alexa Fluor 750 FAB7638S
Allophycocyanin FAB7638A
Biotin FAB7638B
DyLight 350 FAB7638D
DyLight 405 FAB7638E
DyLight 488 FAB7638K
DyLight 550 FAB7638L
DyLight 594 FAB7638M
DyLight 650 FAB7638W
DyLight 680 FAB7638Y
DyLight 755 FAB7638Z
FITC FAB7638F
Janelia Fluor 549 FAB7638I
Janelia Fluor 646 FAB7638J
PerCP FAB7638C

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Bioinformatics

Gene Symbol MILR1
Uniprot