ALDH7A1 Overexpression Lysate

Product Discontinued

ALDH7A1 Overexpression Lysate Summary

• Immunogen: The lysate was created in HEK293T cells, using plasmid ID RC209948 and based on accession number NM_001182. The protein contains a C-terminal DDK tag.
• Epitope: C-terminal Myc/DDK
• Specificity: Homo sapiens aldehyde dehydrogenase 7 family, member A1 (ALDH7A1), mRNA.
• Gene: ALDH7A1

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: This product is intended for use as a positive control in Western Blot.
  
  You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
• Theoretical MW: 55.2 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: RIPA buffer

Lysate Details for Array

• Type: Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for ALDH7A1 Overexpression Lysate

• aldehyde dehydrogenase 7 family, member A1
• Aldehyde dehydrogenase family 7 member A1,26g turgor protein homolog
• Alpha-AASA dehydrogenase
• ATQ1Antiquitin-1
• Betaine aldehyde dehydrogenase
• Delta1-piperideine-6-carboxylate dehydrogenase
• EC 1.2.1
• EC 1.2.1.3
• EC 1.2.1.31
• EC 1.2.1.8
• EPDalpha-aminoadipic semialdehyde dehydrogenase
• FLJ11738
• FLJ92814
• P6c dehydrogenase
• PDEantiquitin-1

Background

Antiquitin is a member of subfamily 7 in the aldehyde dehydrogenase gene family. These enzymes are thought to play a major role in the detoxification of aldehydes generated by alcohol metabolism and lipid peroxidation. This particular member has homology to a previously described protein from the green garden pea, the 26g pea turgor protein. Mutations in this gene cause pyridoxine-dependent epilepsy, which involves a combination of various seizure types and is responsive to immediate administration of pyridoxine hydrochloride. Four additional human antiquitin-like sequences, all of which are pseudogenes, have also been identified. [provided by RefSeq]. Sequence Note: A downstream translation start codon is selected for this RefSeq based on better conservation, and for protein N-terminal consistency with homologous proteins. The use of an upstream start codon, which is present in only a select group of higher mammals, would increase the protein length by 27 aa. This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The extent of this transcript is supported by transcript alignments. CCDS Note: The coding region has been updated to extend the N-terminus to one that is more supported by available transcript and homology data. A downstream translation start codon is selected for this CCDS based on better conservation, and for N-terminal consistency with homologous proteins. The use of an upstream start codon, which is present in only a select group of higher mammals, would increase the protein length by 27 aa. No experimental evidence exists to show which start codon is used.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: ALDH7A1