Recombinant Human AKAP7 His Protein

SDS-Page: Recombinant Human AKAP7 His Protein [NBP1-99000] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

• Reactivity: Hu
• Applications: PAGE
• Concentration: 1 mg/ml

Recombinant Human AKAP7 His Protein Summary

• Description: A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-81 of Human AKAP7
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMGQLCC FPFSRDEGKI SEKNGGEPDD AELVRLSKRL VENAVLKAVQ QYLEETQNKN KPGEGSSVKT EAADQNGNDN ENNRK

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: AKAP7
• Purity: >90%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 11.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH8.0), 10% glycerol, 1 mM DTT, 50 mM NaCl
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: >90%, by SDS-PAGE

Alternate Names for Recombinant Human AKAP7 His Protein

• A kinase (PRKA) anchor protein 7
• AKAP 18
• AKAP15
• AKAP18A-kinase anchor protein 7 isoform alpha
• AKAP-7 isoform gamma
• AKAP-7 isoforms alpha and beta
• A-kinase anchor protein 18 kDa
• A-kinase anchor protein 7 isoform gamma
• A-kinase anchor protein 7 isoforms alpha and beta
• A-kinase anchor protein 7
• A-kinase anchor protein 9 kDa
• A-kinase anchor protein, 18-kD
• A-kinase anchoring protein 18
• protein kinase A anchoring protein 7
• Protein kinase A-anchoring protein 7 isoform gamma
• Protein kinase A-anchoring protein 7 isoforms alpha/beta

Background

AKAP7 (A-kinase anchor protein 7 isoform gamma isoform alpha) , also known as AKAP18, belongs to the A-kinase anchoring protein (AKAP) family, a group of functionally related proteins that bind to a regulatory subunit (RII) of cAMP-dependent protein kinase A (PKA) and target the enzyme to specific subcellular compartments. AKAP 7 is expressed in brain, heart, lung, pancreas and skeletal muscle. AKAP 7 binds PKA to the plasma membrane, and permits functional coupling to the L-type calcium channel. Recombinant human AKAP7 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: AKAP7