Aiolos/IKZF3 Overexpression Lysate (Native)

Images

 
Western Blot: IKZF3 Overexpression Lysate (Adult Normal) [NBL1-11893] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for IKZF3.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

Aiolos/IKZF3 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC207547 and based on accession number NM_012481. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens IKAROS family zinc finger 3 (Aiolos) (IKZF3), transcript variant 1, mRNA.
Gene
IKZF3

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
57.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Aiolos/IKZF3

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for Aiolos/IKZF3 Overexpression Lysate (Native)

  • AIO
  • Aiolos
  • IKAROS family zinc finger 3 (Aiolos)
  • Ikaros family zinc finger protein 3
  • IKZF3
  • zinc finger protein Aiolos
  • ZNFN1A3
  • ZNFN1A3subfamily 1A, 3 (Aiolos)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

AF4984
Species: Hu
Applications: WB, Simple Western, ChIP, CyTOF-ready, ICC, ICFlow
NBP2-34122
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-45374
Species: Hu
Applications: WB, IHC
NBP1-19097
Species: Hu
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP, PLA
AF5165
Species: Hu, Mu
Applications: WB
NB100-56104
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-Fr, IHC-P, IP
20650002
Species: Hu
Applications: WB, ELISA, IHC, IHC-P
NBP1-82689
Species: Hu
Applications: WB, IHC, IHC-P
AF3487
Species: Hu
Applications: WB, IHC, ICC
NBP2-55305
Species: Hu
Applications: WB, ICC/IF
AF231
Species: Hu
Applications: WB, Flow, IHC, IP, CyTOF-ready, ELISA(Cap), ELISA(Det), ICC, ELISA(Sta)
MAB7650
Species: Mu
Applications: WB, IHC
NB100-128
Species: Hu, Mu, Rt
Applications: ChIP, GS, ICC/IF, IHC, IHC-P
NBP2-34099
Species: Hu
Applications: IHC, IHC-P
NBP1-19371
Species: Hu, Mu, Rt, Ca, Rb
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P
AF5117
Species: Hu
Applications: WB, IHC

Publications for Aiolos/IKZF3 Lysate (NBL1-11893) (0)

There are no publications for Aiolos/IKZF3 Lysate (NBL1-11893).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Aiolos/IKZF3 Lysate (NBL1-11893) (0)

There are no reviews for Aiolos/IKZF3 Lysate (NBL1-11893). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Aiolos/IKZF3 Lysate (NBL1-11893) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional Aiolos/IKZF3 Products

Bioinformatics Tool for Aiolos/IKZF3 Lysate (NBL1-11893)

Discover related pathways, diseases and genes to Aiolos/IKZF3 Lysate (NBL1-11893). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Aiolos/IKZF3 Lysate (NBL1-11893)

Discover more about diseases related to Aiolos/IKZF3 Lysate (NBL1-11893).
 

Pathways for Aiolos/IKZF3 Lysate (NBL1-11893)

View related products by pathway.

PTMs for Aiolos/IKZF3 Lysate (NBL1-11893)

Learn more about PTMs related to Aiolos/IKZF3 Lysate (NBL1-11893).
 

Research Areas for Aiolos/IKZF3 Lysate (NBL1-11893)

Find related products by research area.

Blogs on Aiolos/IKZF3

There are no specific blogs for Aiolos/IKZF3, but you can read our latest blog posts.

Customers Who Bought This Also Bought

IL-4 [Unconjugated]
204-IL-010/CF
IL-2 [Unconjugated]
202-IL-010/CF

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Aiolos/IKZF3 Overexpression Lysate (Native) and receive a gift card or discount.

Bioinformatics

Gene Symbol IKZF3
Entrez