AIFM3 Antibody - BSA Free

Western Blot: AIFM3 Antibody [NBP1-76889] - Human brain tissue lysate with AIFM3 antibody at (A) 0.5 and (B) 1 ug/ml.

Immunocytochemistry/ Immunofluorescence: AIFM3 Antibody - BSA Free [NBP1-76889] - Immunofluorescence of AIFM3 in human brain tissue with AIFM3 antibody at 20 ug/mL.

Immunohistochemistry: AIFM3 Antibody - BSA Free [NBP1-76889] - Immunohistochemical staining of human brain tissue using AIFM3 antibody at 2.5 ug/mL.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

AIFM3 Antibody - BSA Free Summary

• Immunogen: Antibody was raised against a 16 amino acid synthetic peptide from near the amino terminus of human AIFM3. The immunogen is located within the first 50 amino acids of AIFM3. Amino Acid Squence: GKGSPRAYQGNGTARH
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: AIFM3
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 20 ug/ml
  • Immunohistochemistry 2.5 ug/ml
  • Immunohistochemistry-Paraffin 2.5 ug/ml
  • Western Blot 0.5-1 ug/ml
• Control Peptide:

  AIFM3 Peptide (NBP1-76889PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for AIFM3 Antibody - BSA Free

• AIFLFLJ30473
• apoptosis-inducing factor 3
• apoptosis-inducing factor like
• apoptosis-inducing factor, mitochondrion-associated, 3
• Apoptosis-inducing factor-like protein
• FLJ45137

Background

Apoptosis, also known as programmed cell death, plays major roles in development and normal tissue turnover in addition to tumor formation. Recently a protein similar to the apoptosis-inducing factor (AIF) was cloned and designated AIFL (also known as AIFM3). AIFM3 is expressed ubiquitously and is predominantly localized to the inner membranes of mitochondria. Unlike AIF, AIFM3 does not translocate to the nucleus upon induction of apoptosis. However, overexpression of AIFM3, like AIF, induced cytochrome c release from the mitochondria, cleavage of caspase 3, and ultimately apoptosis, indicating AIFM3 induces apoptosis through caspase activation. Multiple isoforms of AIFM3 are known to exist.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for AIFM3 Antibody (NBP1-76889)(1)

Publication using NBP1-76889

• Wang J, Rattner A, Nathans J Bacterial meningitis in the early postnatal mouse studied at single-cell resolution eLife 2023-06-15 [PMID: 37318981] (IHC, Mouse)

Bioinformatics

• Gene Symbol: AIFM3
• Uniprot:
  • Q96NN9(Human)