AHSA2 Antibody

Immunocytochemistry/Immunofluorescence: AHSA2 Antibody [NBP2-12736] - =Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-AHA2 Polyclonal Antibody at 1:80 for 12 hours at 4C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Nucleus. Cytoplasm. Magnification: 100x. Heat Shocked at 42C for 1h.

Immunocytochemistry/Immunofluorescence: AHSA2 Antibody [NBP2-12736] - Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-AHA2 Polyclonal Antibody at 1:80 for 12 hours at 4C. Secondary Antibody: R-PE Goat Anti-Rabbit (yellow) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Nucleus. Cytoplasm. Magnification: 20x. Heat Shocked at 42C for 1h.

Product Discontinued

AHSA2 Antibody Summary

• Immunogen: Aha2
• Specificity: Cross reacts with Aha1.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: AHSA2P
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot 1:100-1:2000
• Application Notes: 1 ug/ml of AHA2 Antibody was sufficient for detection of Aha2 in 20 ug of rat tissue lysate by colorimetric immunoblot analysis using Goat anti-rat IgG:HRP as the secondary Antibody.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4), 50% Glycerol
• Preservative: 0.09% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for AHSA2 Antibody

• activator of 90 kDa heat shock protein ATPase homolog 2
• AHA1, activator of heat shock 90kDa protein ATPase homolog 2 (yeast)
• DKFZp564C236
• FLJ34679
• FLJ41715
• Hch1

Background

Certificate of Analysis, 1 ug/mL was sufficient for detection of AHSA2 in 20ug of rat tissue lysate by colorimetric immunoblot analysis using Goat anti-rat IgG: HRP as the secondary antibody.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: AHSA2P
• Uniprot:
  • NP_689605.1(Human)