AHI1 Antibody

Flow Cytometry: AHI1 Antibody [NBP2-76427] - Analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation overnight (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Product Discontinued

AHI1 Antibody Summary

• Immunogen: Peptide with sequence PTAESEAKVKTC corresponding to N-Terminus according to NP_060121.3, NP_001128303.1, NP_001128302.1, NP_001128304.1.
• Specificity: This antibody is expected to recognise both reported isoforms. Variants ( NP_060121.3; NP_001128303.1; NP_001128302.1) encode the same isofom.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: AHI1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 ug/mL
  • Peptide ELISA Detection limit 1:16000
• Application Notes: WB: Preliminary experiments gave a consistent band at approx 75-80 kDa in Human Cerebellum and Frontal Cortex lysates after 0.5 ug/mL antibody staining and was successfully blocked by incubation with the immunizing peptide. Please note that currently we cannot find an explanation in the literature for this band, given the calculated size of 137 kDa according to NP_060121.3

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for AHI1 Antibody

• Abelson helper integration site 1 protein homolog
• Abelson helper integration site 1
• Abelson helper integration site
• AHI-1
• contatins SH3 and WD40 domains
• dJ71N10.1
• DKFZp686J1653
• FLJ14023
• FLJ20069
• JBTS3
• jouberin
• ORF1

Background

Jouberin (AHI1), encoding the Jouberin protein, is an alternatively spliced signaling molecule that contains seven Trp-Asp (WD) repeats, an SH3 domain, and numerous SH3-binding sites. The gene is expressed strongly in embryonic hindbrain and forebrain, and AHI1 may be required for both cerebellar and cortical development in humans. Defects in AHI1 are the cause of Joubert syndrome 3, an autosomal recessive brain congenital malformation. The exact function of the AHI1 gene is not yet known.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: AHI1