AGAP1 Antibody (OTI4E8)

Western Blot: AGAP1 Antibody (OTI4E8) [NBP2-01708] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AGAP1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-AGAP1.

Immunohistochemistry-Paraffin: AGAP1 Antibody (OTI4E8) [NBP2-01708] - Staining of paraffin-embedded Human tonsil using anti-AGAP1 mouse monoclonal antibody.

Flow Cytometry: AGAP1 Antibody (OTI4E8) [NBP2-01708] - Analysis of Jurkat cells, using anti-AGAP1 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, IHC
• Clone: OTI4E8
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

AGAP1 Antibody (OTI4E8) Summary

• Immunogen: Full length human recombinant protein of human AGAP1(NP_055729) produced in HEK293T cell.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: AGAP1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:2000

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for AGAP1 Antibody (OTI4E8)

• AGAP-1
• ANK repeat and PH domains 1
• ArfGAP with GTPase domain, ankyrin repeat and PH domain 1
• centaurin, gamma 2
• centaurin-gamma-2
• CENTG2
• cnt-g2
• GTP-binding and GTPase-activating protein 1
• KIAA1099arf-GAP with GTPase, ANK repeat and PH domain-containing protein 1
• MGC71657

Background

CENTG2 belongs to an ADP-ribosylation factor GTPase-activating (ARF-GAP) protein family involved in membrane traffic and actin cytoskeleton dynamics (Nie et al., 2002 [PubMed 12388557]).[supplied by OMIM].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: AGAP1
• Entrez:
  • 116987(Human)