AFF4 Antibody

Western Blot: AFF4 Antibody [H00027125-D01P] - Analysis of AFF4 expression in mouse liver.

Immunocytochemistry/Immunofluorescence: AFF4 Antibody [H00027125-D01P] - Analysis of antibody to AFF4 on HeLa cell. Antibody concentration 10 ug/ml.

Western Blot: AFF4 Antibody [H00027125-D01P] - Analysis of AFF4 expression in human liver.

Western Blot: AFF4 Antibody [H00027125-D01P] - Analysis of AFF4 expression in transfected 293T cell line by AFF4 polyclonal antibody.Lane 1: AFF4 transfected lysate(40.20 KDa).Lane 2: Non-transfected lysate.

Product Discontinued

AFF4 Antibody Summary

• Immunogen: AFF4 (AAH25700.1, 1 a.a. - 365 a.a.) full-length human protein. MNREDRNVLRMKERERRNQEIQQGEDAFPPSSPLFAEPYKVTSKEDKLSSRIQSMLGNYDEMKDFIGDRSIPKLVAIPKPTVPPSADEKSNPNFFEQRHGGSHQSSKWTPVGPAPSTSQSQKRSSGLQSGHSSQRTSAGSSSGTNSSGQRHDRESYNNSGSSSRKKGQHGSEHSKSRSSSPGKPQAVSSLNSSHSRSHGNDHHSKEHQRSKSPRDPDANWDSPSRVPFSSGQHSTQSFPPSLMSKSNSMLQKPTAYVRPMDGQESMEPKLSSEHYSSQSHGNSMTELKPSSKAHLTKLKIPSQPLDASASGDVSCVDEILKEMTHSWPPPLTAIHTPCKTEPSKFPFPTKESQQSNFGTGEQRLK
• Specificity: AFF4 - AF4/FMR2 family, member 4,
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: AFF4
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot 1:500
• Application Notes: Antibody reactive against Recombinant Protein with GST tag on ELISA and Western Blot and also on transfected lysate in western blot. GST tag alone is used as a negative control.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: No Preservative
• Purity: Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for AFF4 Antibody

• AF4/FMR2 family, member 4
• AF5Q31AF4/FMR2 family member 4
• ALL1-fused gene from chromosome 5q31 protein
• Major CDK9 elongation factor-associated protein
• MCEFALL1 fused gene from 5q31
• MGC75036
• Protein AF-5q31

Background

AFF4, or AF4/FMR2 family member 4, contains a 127 kDa, 98 kDa, and 39 kDa isoform, and is involved in the process of improving the catalytic rate of RNA polymerase II transcription. Disease research is currently being conducted with AFF4 on its relation to acute lymphoblastic leukemia, as well as other types of leukemia. This protein interacts with SIAH1, CCNT1, MLLT3, MLLT1, and MED10 during the regulation of DNA-dependent transcription and the transcription of RNA polymerase II.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: AFF4
• Entrez:
  • 27125(Human)
• Uniprot:
  • AAH25700.1(Human)