ADAM8 Antibody (143303) [CoraFluor™ 1]

• Reactivity: Hu
• Applications: WB, IP
• Clone: 143303
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: CoraFluor 1

ADAM8 Antibody (143303) [CoraFluor™ 1] Summary

• Description: CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(TM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
  
  CoraFluor(TM) 1, amine reactive
  
  CoraFluor(TM) 1, thiol reactive
  
  For more information, please see our CoraFluor(TM) TR-FRET technology flyer.
• Immunogen: Mouse myeloma cell line NS0-derived recombinant human ADAM8 ectodomain
  Asp158-Ser653
  Accession # P78325
• Specificity: Detects human ADAM8 in direct ELISAs and Western blots. In Western blots, less than 5% cross-reactivity with the ectodomains of recombinant human ADAM9, 10, 15, 17, and 19 is observed. Detects an epitope within aa 201‑497 of recombinant human ADAM8 which corresponds to the metalloprotease and disintegrin-like domains.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: ADAM8
• Purity: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark. Do not freeze.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein A or G purified from hybridoma culture supernatant

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for ADAM8 Antibody (143303) [CoraFluor™ 1]

• a disintegrin and metalloproteinase domain 8
• ADAM 8
• ADAM metallopeptidase domain 8
• ADAM8
• CD156a antigen
• CD156a
• CD156human leukocyte differentiation antigen
• Cell surface antigen MS2
• EC 3.4.24
• EC 3.4.24.-
• MGC134985
• MS2
• MS2disintegrin and metalloproteinase domain-containing protein 8

Background

Members of ADAM family are cell surface proteins with a unique structure possessing both potential adhesion and protease domains. The extracellular region of ADAM8 shows significant amino acid sequence homology to hemorrhagic snake venom proteins, including the metalloprotease and disintegrin domains. The expression of ADAM8 is upregulated by retinoic acid and vitamin D3. ADAM8 is involved in protein processing at the cell surface and is also thought to act as a leukocyte differentiation marker.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ADAM8