| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | IC808P | |||
| Carrier Free | AF808-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF1433-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-89062SS | |||
| Azide and BSA Free | NBP2-80895 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB1433-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | IC14331P | |||
| Carrier Free | IC14331A | |||
| Carrier Free | IC14331F | |||
| Carrier Free | MAB14331-SP | |||
| Carrier Free | MAB14331-500 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89952-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-73165 | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor Plus 405 | NBP3-49523AFP405 | |||
| Alexa Fluor Plus 488 | NBP3-49523AFP488 | |||
| Alexa Fluor Plus 555 | NBP3-49523AFP555 | |||
| Alexa Fluor Plus 594 | NBP3-49523AFP594 | |||
| Alexa Fluor Plus 647 | NBP3-49523AFP647 | |||
| Alexa Fluor Plus 680 | NBP3-49523AFP680 | |||
![Aging results in diminished OPN production in response to 2-20 RSV infection.Young and aged BALB/c mice (n = 4/group) were intranasally infected with a dose of 106 pfu/mouse of A2 or 105 pfu/mouse of 2-20) and total lung RNA was collected on time points indicated. (A-B) RNA transcripts of OPN were analyzed with qRT-PCR and represented as a relative ratio of target gene expression to endogenous mouse HPRT and examined with rA2-L19F, 2-20, or A2. (B) 5 µm lung sections obtained at 4, 5 and 8 dpi with either rA2-L19F, 2-20, or A2 infected young and aged mice. Lung sections were immunostained for anti-mouse OPN and nickel-DAB reagent before counterstaining with hematoxylin and eosin. Representative images shown are at 100x magnification with inset [400x] displaying nickel-DAB (dark brown/black staining) positive cells and contrast the hematoxylin (light blue) nuclear stain. Representative images are shown with scale bar indicating 100 µm. (C) Enumeration of OPN-positive cells was performed with ImmunoRatio ImageJ analysis on 200X magnified lung sections from 8 dpi and values are shown as a percentage of total hematoxylin-stained cells in an individual box plot with mean interval bars. Within a single frame, at least 5 frames per mouse (n = 4/group) were collected and individual dot plots are shown of either aged or young mice with interval bars and significance, determined with ANOVA and Fisher's test (p<0.05). (D) qRT-PCR was performed on total lung RNA from young and aged 2–20 RSV infected mice for mRNA expression of OPN receptor CD44. Statistical significance was determine with ANOVA 2-way analysis with p<0.05. All experiments were performed in triplicate. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/24558422), licensed under a CC-BY license. Not internally tested by R&D Systems.](http://images.novusbio.com/fullsize/af808_mouse-osteopontin-opn-affinity-purified-polyclonal-ab-812025557278.jpg)
![Western Blot: Osteopontin/OPN Antibody (1B20) - BSA Free [NB110-89062] - Analysis of Osteopontin expression in U2OS whole cell lysate.](http://images.novusbio.com/fullsize/Osteopontin-OPN-Antibody-1B20-BSA-Free-Western-Blot-NB110-89062-img0007.jpg "Western Blot: Osteopontin/OPN Antibody (1B20) - BSA Free [NB110-89062] - Analysis of Osteopontin expression in U2OS whole cell lysate.")
![Immunocytochemistry/Immunofluorescence: Osteopontin/OPN Antibody (1B20) - BSA Free [NB110-89062] - Osteopontin antibody was tested at 1:50 in U2OS cells with FITC (green). Nuclei and actin were counterstained with Dapi (blue) and Phalloidin (red).](http://images.novusbio.com/fullsize/Osteopontin-OPN-Antibody-1B20-BSA-Free-Immunocytochemistry-Immunofluorescence-NB110-89062-img0008.jpg)
![Immunohistochemistry: Osteopontin/OPN Antibody (1B20) - BSA Free [NB110-89062] - Analysis of Osteopontin on human lung adenocarcinoma.](http://images.novusbio.com/fullsize/Osteopontin-OPN-Antibody-1B20-BSA-Free-Immunohistochemistry-NB110-89062-img0009.jpg)
![Simple Western: Osteopontin/OPN Antibody (1B20) - BSA Free [NB110-89062] - Simple Western lane view shows a specific band for Osteopontin in 0.5 mg/ml of Human Breast Cancer lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. * Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody](http://images.novusbio.com/fullsize/Osteopontin-OPN-Antibody-1B20-BSA-Free-Simple-Western-NB110-89062-img0010.jpg)
![Immunohistochemistry: Osteopontin/OPN Antibody (1B20) - BSA Free [NB110-89062] - Photomicrographs of immunostaining for osteocalcin & osteopontin in (A) Group I, (B) Group II (C) Group III at 6 weeks.The pictures are arranged by staining technique (columns) & the investigated treatment (rows). Areas that stained positive for osteocalcin & osteopontin are indicated by red arrowheads. NB, new bone; OS, osteoid; FM, fatty marrow. Image collected & cropped by CiteAb from the following publication (//peerj.com/articles/3513), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-89062_mouse-monoclonal-osteopontin-opn-antibody-1b20-3102024153560.jpg)
![Immunohistochemistry: Osteopontin/OPN Antibody (1B20) - BSA Free [NB110-89062] - Photomicrographs of immunostaining for osteocalcin & osteopontin in (A) Group I, (B) Group II (C) Group III at 6 weeks.The pictures are arranged by staining technique (columns) & the investigated treatment (rows). Areas that stained positive for osteocalcin & osteopontin are indicated by red arrowheads. NB, new bone; OS, osteoid; FM, fatty marrow. Image collected & cropped by CiteAb from the following publication (//peerj.com/articles/3513), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-89062_mouse-monoclonal-osteopontin-opn-antibody-1b20-31020241534321.jpg)
![Western Blot: Osteopontin/OPN Antibody (1B20) - BSA Free [NB110-89062] - Photomicrographs of immunostaining for osteocalcin & osteopontin in (A) Group I, (B) Group II (C) Group III at 6 weeks.The pictures are arranged by staining technique (columns) & the investigated treatment (rows). Areas that stained positive for osteocalcin & osteopontin are indicated by red arrowheads. NB, new bone; OS, osteoid; FM, fatty marrow. Image collected & cropped by CiteAb from the following publication (//peerj.com/articles/3513), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb110-89062_mouse-monoclonal-osteopontin-opn-antibody-1b20-310202415394515.jpg)
![Western Blot: Osteopontin/OPN Antibody [NB600-1043] - Western Blot of Osteopontin/OPN/OPN antibody. Lane 1: Molecular Weight Marker. Lane 2: Human Osteopontin/OPN [250ng]. Lane 3: MMP-cleaved Osteopontin/OPN. Primary Antibody: Anti-Osteopontin/OPN at 1:1000 ovenight at 2-8C. Secondary Antibody: Goat Anti-Rabbit IgG HRP at 1:10,000. Observed MW: full length 66kDa, MMP-cleaved 32kDa.](http://images.novusbio.com/fullsize/Osteopontin-OPN-Antibody-Western-Blot-NB600-1043-img0009.jpg "Western Blot: Osteopontin/OPN Antibody [NB600-1043] - Western Blot of Osteopontin/OPN/OPN antibody. Lane 1: Molecular Weight Marker. Lane 2: Human Osteopontin/OPN [250ng]. Lane 3: MMP-cleaved Osteopontin/OPN. Primary Antibody: Anti-Osteopontin/OPN at 1:1000 ovenight at 2-8C. Secondary Antibody: Goat Anti-Rabbit IgG HRP at 1:10,000. Observed MW: full length 66kDa, MMP-cleaved 32kDa.")
![Immunohistochemistry: Osteopontin/OPN Antibody [NB600-1043] - Osteopontin/OPN/OPN antibody was used at a 1:100-1:300 dilution to detect osteopontin by immunohistochemistry. Osteopontin/OPN is a normal component of elastic fibers in the breast (shown heavily stained in this section of human breast tumor cells). There is also weak staining of the extracellular matrix. Osteopontin/OPN is not expressed in breast tumor cells, and there is no staining of the breast cells in this section. No antigen retrieval is required.](http://images.novusbio.com/fullsize/Osteopontin-OPN-Antibody-Immunohistochemistry-NB600-1043-img0010.jpg)
![Osteopontin/OPN Antibody [NB600-1043]](http://images.novusbio.com/fullsize/Osteopontin-OPN-Antibody-N-A-NB600-1043-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: Osteopontin/OPN Antibody [NBP1-89952] - Staining of human cell line Hep G2 shows localization to the Golgi apparatus. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Osteopontin-OPN-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89952-img0012.jpg "Immunocytochemistry/Immunofluorescence: Osteopontin/OPN Antibody [NBP1-89952] - Staining of human cell line Hep G2 shows localization to the Golgi apparatus. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: Osteopontin/OPN Antibody [NBP1-89952] - Staining of human placenta shows high expression.](http://images.novusbio.com/fullsize/Osteopontin-OPN-Antibody-Immunohistochemistry-Paraffin-NBP1-89952-img0007.jpg)
![Immunohistochemistry-Paraffin: Osteopontin/OPN Antibody [NBP1-89952] - Staining of human liver shows low positivity in hepatocytes as expected.](http://images.novusbio.com/fullsize/Osteopontin-OPN-Antibody-Immunohistochemistry-Paraffin-NBP1-89952-img0016.jpg)
![Immunohistochemistry-Paraffin: Osteopontin/OPN Antibody [NBP1-89952] - Staining of human tonsil shows low positivity in non-germinal center cells as expected.](http://images.novusbio.com/fullsize/Osteopontin-OPN-Antibody-Immunohistochemistry-Paraffin-NBP1-89952-img0017.jpg)
![Western Blot: Osteopontin/OPN Antibody [NB100-1883] - Staining of lysates of cell line MOLT4 (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Osteopontin-OPN-Antibody-Western-Blot-NB100-1883-img0001.jpg "Western Blot: Osteopontin/OPN Antibody [NB100-1883] - Staining of lysates of cell line MOLT4 (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Western Blot: Osteopontin/OPN Antibody [NBP1-59190] - Jurkat cell lysate, Antibody Titration: 1.25ug/ml](http://images.novusbio.com/fullsize/Osteopontin-OPN-Antibody-Western-Blot-NBP1-59190-img0002.jpg "Western Blot: Osteopontin/OPN Antibody [NBP1-59190] - Jurkat cell lysate, Antibody Titration: 1.25ug/ml")
![Immunohistochemistry-Paraffin: Osteopontin/OPN Antibody [NBP1-59190] - Human kidney Tissue, antibody concentration 4-8ug/ml. Cells with positive label: renal corpuscle cells (indicated with arrows) 400X magnification.](http://images.novusbio.com/fullsize/Osteopontin-OPN-Antibody-Immunohistochemistry-Paraffin-NBP1-59190-img0003.jpg)
![Western Blot: Osteopontin/OPN Antibody [NBP1-59190] - Sample Tissue: Jurkat, Lane A: Primary Antibody, Lane B: Primary Antibody + Blocking Peptide, Primary Antibody Concentration: 2.5ug/mL, Peptide Concentration: 2.0ug/mL, Lysate Quantity: 25ug/lane, Gel Concentration: 12%](http://images.novusbio.com/fullsize/Osteopontin-OPN-Antibody-Western-Blot-NBP1-59190-img0004.jpg)
![Western Blot: Osteopontin/OPN Antibody (5E4) [NBP2-45954] - Analysis of HEK293T cells were transiently transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Osteopontin.](http://images.novusbio.com/fullsize/Osteopontin-OPN-Antibody-5E4-Western-Blot-NBP2-45954-img0009.jpg "Western Blot: Osteopontin/OPN Antibody (5E4) [NBP2-45954] - Analysis of HEK293T cells were transiently transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Osteopontin.")
![Immunohistochemistry: Osteopontin/OPN Antibody (5E4) [NBP2-45954] - Analysis of Carcinoma of Human prostate tissue using SPP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Osteopontin-OPN-Antibody-5E4-Immunohistochemistry-NBP2-45954-img0008.jpg)
![Immunohistochemistry: Osteopontin/OPN Antibody (5E4) [NBP2-45954] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Osteopontin-OPN-Antibody-5E4-Immunohistochemistry-NBP2-45954-img0001.jpg)
![Immunohistochemistry: Osteopontin/OPN Antibody (5E4) [NBP2-45954] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Osteopontin-OPN-Antibody-5E4-Immunohistochemistry-NBP2-45954-img0002.jpg)
![Immunohistochemistry: Osteopontin/OPN Antibody (5E4) [NBP2-45954] - Analysis of Human lung tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Osteopontin-OPN-Antibody-5E4-Immunohistochemistry-NBP2-45954-img0003.jpg)
![Immunohistochemistry: Osteopontin/OPN Antibody (5E4) [NBP2-45954] - Analysis of Carcinoma of Human lung tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Osteopontin-OPN-Antibody-5E4-Immunohistochemistry-NBP2-45954-img0004.jpg)
![Immunohistochemistry: Osteopontin/OPN Antibody (5E4) [NBP2-45954] - Analysis of Carcinoma of Human pancreas tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Osteopontin-OPN-Antibody-5E4-Immunohistochemistry-NBP2-45954-img0005.jpg)
![Immunohistochemistry: Osteopontin/OPN Antibody (5E4) [NBP2-45954] - Analysis of Human thyroid tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Osteopontin-OPN-Antibody-5E4-Immunohistochemistry-NBP2-45954-img0006.jpg)
![Immunohistochemistry: Osteopontin/OPN Antibody (5E4) [NBP2-45954] - Analysis of Human prostate tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/Osteopontin-OPN-Antibody-5E4-Immunohistochemistry-NBP2-45954-img0007.jpg)
