| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | IC1924A | |||
| Carrier Free | NL1924R | |||
| Carrier Free | AF1924-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | NL20181G | |||
| Carrier Free | AF2018-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF5507-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-47434-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB5507-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB55071-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-98886-50ul | |||
![Soluble factors increase the numbers of Hes3+ cells in the adult rat hippocampus.(a) Treatment with the soluble factors induced a powerful increase in the number of Sox2+ cells in the hilus [Quantifications are from images taken from the tip of the hilus to approximately 1,300 micrometers towards the CA3 region – representative images are shown. Staining for the young neuron marker doublecortin (DCX) helps to visualize the subgranular zone]. (b) The same treatment also induced an increase in the numbers of Hes3+ cells in the hilus. (c) Sox2+ cells inside the hilus co-expressed Hes3; in contrast, Sox2+ cells in the subgranular zone did not co-express Hes3. (d,e) Quantification graphs of the increases in Sox2+ and Hes3+ cell numbers in the hilus (p0.05; *, p0.05, #, p C) in exon 2 of the SMPD1 gene. D) Flow cytometry analysis of pluripotency protein markers: NANOG and TRA-1–60. E) RT-PCR verification for the clearance of the Sendai virus from reprogrammed cells. Sendai virus vector transduced fibroblasts were used as a positive control. F) Pathological analysis of teratoma from TRNDi009-C iPSC, showing a normal ectodermal, mesodermal, and endodermal differentiation. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31132580), licensed under a CC-BY license. Not internally tested by R&D Systems.](http://images.novusbio.com/fullsize/mab2018_human-mouse-rat-sox2-mab-clone-245610-22820241723619.jpg)
![Characterization of cell lines.(A) H&E staining of tumor 5653, the arrow indicates the location of the tumor. The right panel shows a higher magnification. (B) Genotyping PCR on genomic wildtype mouse, genomic NPcis mouse (heterozygous for Nf1 and Tp53) DNA and DNA extracted from cell lines 17, 5653 and 5746. The glioma cell lines show complete loss of the wildtype allele for Nf1 and Tp53. (C) Western blot shows the expression of markers consistent with OPC lineage origin of our cell lines. SOX2 is expressed in Neural Progenitor cells (NPC) and Oligodendrocyte precursor cells (OPC); NG2 is expressed in OPCs. Markers (CPNase and MAG) expressed in pre-oligodendrocytes (pre-OL) or mature oligodendrocytes (OL) are not present, highlighting the progenitor status of our lines [22]. (D) The ATRX mutation (E2281*) present in TM31 was confirmed using sequencing. Top shows the reference genome DNA and protein sequence, bottom shows the TM31 sequence with STOP mutation. (E) The homozygous 4bp NF1 deletion (p.LF1247fs*18) present in TM31 was confirmed using sequencing. Top shows the reference genome DNA and protein sequence, bottom shows the TM31 sequence with the homozygous 4bp deletion inducing a frameshift. (F) Western blot for TP53 and CDKN2A, showing that both genes are absent in TM31, but not in glioma line LN319. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36730269), licensed under a CC-BY license. Not internally tested by R&D Systems.](http://images.novusbio.com/fullsize/mab2018_human-mouse-rat-sox2-mab-clone-245610-western-blot-1212202596399.jpg)
![Characterization of cell lines.(A) H&E staining of tumor 5653, the arrow indicates the location of the tumor. The right panel shows a higher magnification. (B) Genotyping PCR on genomic wildtype mouse, genomic NPcis mouse (heterozygous for Nf1 and Tp53) DNA and DNA extracted from cell lines 17, 5653 and 5746. The glioma cell lines show complete loss of the wildtype allele for Nf1 and Tp53. (C) Western blot shows the expression of markers consistent with OPC lineage origin of our cell lines. SOX2 is expressed in Neural Progenitor cells (NPC) and Oligodendrocyte precursor cells (OPC); NG2 is expressed in OPCs. Markers (CPNase and MAG) expressed in pre-oligodendrocytes (pre-OL) or mature oligodendrocytes (OL) are not present, highlighting the progenitor status of our lines [22]. (D) The ATRX mutation (E2281*) present in TM31 was confirmed using sequencing. Top shows the reference genome DNA and protein sequence, bottom shows the TM31 sequence with STOP mutation. (E) The homozygous 4bp NF1 deletion (p.LF1247fs*18) present in TM31 was confirmed using sequencing. Top shows the reference genome DNA and protein sequence, bottom shows the TM31 sequence with the homozygous 4bp deletion inducing a frameshift. (F) Western blot for TP53 and CDKN2A, showing that both genes are absent in TM31, but not in glioma line LN319. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36730269), licensed under a CC-BY license. Not internally tested by R&D Systems.](http://images.novusbio.com/fullsize/mab2018_human-mouse-rat-sox2-mab-clone-245610-western-blot-121220259161717.jpg)
![Western Blot: HMGB3/HMG4 Antibody [NBP2-47434] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG.](http://images.novusbio.com/fullsize/HMGB3-HMG4-Antibody-Western-Blot-NBP2-47434-img0005.jpg "Western Blot: HMGB3/HMG4 Antibody [NBP2-47434] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG.")
![Immunocytochemistry/Immunofluorescence: HMGB3/HMG4 Antibody [NBP2-47434] - Immunofluorescent staining of human cell line MCF7 shows localization to nucleus & nucleoli.](http://images.novusbio.com/fullsize/HMGB3-HMG4-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-47434-img0006.jpg)
![Immunohistochemistry-Paraffin: HMGB3/HMG4 Antibody [NBP2-47434] - Staining of human pancreas shows no positivity as expected.](http://images.novusbio.com/fullsize/HMGB3-HMG4-Antibody-Immunohistochemistry-Paraffin-NBP2-47434-img0013.jpg)
![Immunohistochemistry-Paraffin: HMGB3/HMG4 Antibody [NBP2-47434] - Staining of human pancreas shows low expression as expected.](http://images.novusbio.com/fullsize/HMGB3-HMG4-Antibody-Immunohistochemistry-Paraffin-NBP2-47434-img0008.jpg)
![Immunohistochemistry-Paraffin: HMGB3/HMG4 Antibody [NBP2-47434] - Staining of human placenta shows high expression.](http://images.novusbio.com/fullsize/HMGB3-HMG4-Antibody-Immunohistochemistry-Paraffin-NBP2-47434-img0009.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: HMGB3/HMG4 Antibody [NBP2-47434] - Analysis in human placenta and skeletal muscle tissues using NBP2-47434 antibody. Corresponding HMGB3 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/HMGB3-HMG4-Antibody-Immunohistochemistry-Paraffin-NBP2-47434-img0011.jpg)
![Immunohistochemistry-Paraffin: HMGB3/HMG4 Antibody [NBP2-47434] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/HMGB3-HMG4-Antibody-Immunohistochemistry-Paraffin-NBP2-47434-img0012.jpg)
![Immunohistochemistry-Paraffin: HMGB3/HMG4 Antibody [NBP2-47434] - Staining of human skin shows no positivity in squamous epithelial cells as expected.](http://images.novusbio.com/fullsize/HMGB3-HMG4-Antibody-Immunohistochemistry-Paraffin-NBP2-47434-img0014.jpg)
![Western Blot: HMGB3/HMG4 Antibody [NBP2-24645] - Analysis of HMGB3 in mouse placenta lysate in the 1) absence and 2) presence of immunizing peptide using this antibody at 3 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/HMGB3-HMG4-Antibody-Western-Blot-NBP2-24645-img0002.jpg "Western Blot: HMGB3/HMG4 Antibody [NBP2-24645] - Analysis of HMGB3 in mouse placenta lysate in the 1) absence and 2) presence of immunizing peptide using this antibody at 3 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Western Blot: HMGB3/HMG4 Antibody [NBP2-24512] - Analysis of HMGB3 in mouse placenta lysate in the 1) absence and 2) presence of immunizing peptide using NBP2-24512 at 0.5 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize/HMGB3-HMG4-Antibody-Western-Blot-NBP2-24512-img0002.jpg "Western Blot: HMGB3/HMG4 Antibody [NBP2-24512] - Analysis of HMGB3 in mouse placenta lysate in the 1) absence and 2) presence of immunizing peptide using NBP2-24512 at 0.5 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.")
![Western Blot: HMGB3/HMG4 Antibody (S01-9C9) [NBP3-15041] - Western blot detection of HMGB3/HMG4 in K562, C6, 3T3, Hela cell lysates using NBP3-15041 (1:1000 diluted). Predicted band size: 23kDa. Observed band size: 23kDa.](http://images.novusbio.com/fullsize/nbp3-15041_rabbit-hmgb3-hmg4-mab-s01-9c9-23420241545359.jpg "Western Blot: HMGB3/HMG4 Antibody (S01-9C9) [NBP3-15041] - Western blot detection of HMGB3/HMG4 in K562, C6, 3T3, Hela cell lysates using NBP3-15041 (1:1000 diluted). Predicted band size: 23kDa. Observed band size: 23kDa.")
![Western Blot: HMGB3/HMG4 Antibody (JE63-39) [NBP3-32437] - Western blot analysis of HMGB3/HMG4 on different lysates with Rabbit anti-HMGB3/HMG4 antibody (NBP3-32437) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: 293T cell lysate Lane 3: MCF7 cell lysate Lane 4: Jurkat cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 23 kDa Observed band size: 23 kDa Exposure time: 50 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32437) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32437_rabbit-hmgb3-hmg4-mab-je63-39-27820241631669.jpg "Western Blot: HMGB3/HMG4 Antibody (JE63-39) [NBP3-32437] - Western blot analysis of HMGB3/HMG4 on different lysates with Rabbit anti-HMGB3/HMG4 antibody (NBP3-32437) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: 293T cell lysate Lane 3: MCF7 cell lysate Lane 4: Jurkat cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 23 kDa Observed band size: 23 kDa Exposure time: 50 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32437) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry-Paraffin: HMGB3/HMG4 Antibody [NBP2-98886] - Immunochemical staining of human HMGB3/HMG4 in human placenta with rabbit polyclonal antibody (1:2000, formalin-fixed paraffin embedded sections).](http://images.novusbio.com/fullsize/HMGB3-HMG4-Antibody-Immunohistochemistry-Paraffin-NBP2-98886-img0001.jpg "Immunohistochemistry-Paraffin: HMGB3/HMG4 Antibody [NBP2-98886] - Immunochemical staining of human HMGB3/HMG4 in human placenta with rabbit polyclonal antibody (1:2000, formalin-fixed paraffin embedded sections).")