NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for
Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox">

NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for
Fluorescent ICC Staining of Cells on Coverslips." alt="Bromodeoxyuridine/BrdU was detected in immersion fixed MCF-7 human breast cancer cell line stimulated with BrdU using Mouse Anti-Bromodeoxyuridine/ BrdU Antigen Affinity-purified Monoclonal Antibody (Catalog # MAB7225) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog #
NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for
Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />
VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (
CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell nuclei. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox">

VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (
CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell nuclei. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />
VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (
CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell nuclei. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox">

VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (
CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell nuclei. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />
MAB003, open histo-gram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #
F0101B). To facilitate intracellular staining, cells were fixed with cold, 70% ethanol for 5 minutes, DNA was denatured with 1.5M HCl for 30 minutes, and then cells were permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog #
FC005)." class="big_lightbox">

MAB003, open histo-gram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #
F0101B). To facilitate intracellular staining, cells were fixed with cold, 70% ethanol for 5 minutes, DNA was denatured with 1.5M HCl for 30 minutes, and then cells were permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog #
FC005)." class="big_thumb" />