Bromodeoxyuridine/BrdU Antibody (MoBu-1)

Images

 
Immunohistochemistry-Paraffin: Bromodeoxyuridine/BrdU Antibody (MoBu-1) [NB500-439] - Mouse Small Intestine stained with BrdU Monoclonal Antibody (MoBu-1).
Immunohistochemistry: Bromodeoxyuridine/BrdU Antibody (MoBu-1) [NB500-439] - Staining of bromodeoxyuridine/BrdU-labeled cells (chick embryo; paraffin-embedded sections)

Product Details

Summary
Reactivity All-NASpecies Glossary
Applications Flow, ICC/IF, IHC
Clone
MoBu-1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

Bromodeoxyuridine/BrdU Antibody (MoBu-1) Summary

Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-68041)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Antibody is stable for 24 months. Non-hazardous.
Immunogen
Bromodeoxyuridine/BrdU conjugated to hemocyanine (isolated from Helix pomatia)
Localization
Nuclear
Marker
Proliferation Marker
Specificity
It reacts with Bromodeoxyuridine (BrdU) in single stranded DNA (produced by partial denaturation of double stranded DNA), BrdU coupled to a protein carrier, as well as free BrdU. BrdU is a thymidine analog, incorporated into cell nuclei during DNA synthesis prior to mitosis. Antibody to BrdU is helpful in detecting S-phase cells, providing useful information on the aggressiveness of tumors.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1-2 ug/million cells
  • Immunocytochemistry/ Immunofluorescence 1-2 ug/ml
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. For staining of formalin-fixed tissues, incubate sections in 4N HCl for 30 minutes at RT followed by digestion with trypsin at 1mg/ml PBS, 10 min at 37C.
Optimal dilution for a specific application should be determined.
Publications
Read Publications using
NB500-439 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Alternate Names for Bromodeoxyuridine/BrdU Antibody (MoBu-1)

  • 5-bromo-2-deoxyuridine
  • BrdU
  • Bromodeoxyuridine
  • BUdr

Background

Bromodexyuridine (BrdU) is a thymidine analog which is selectively incorporated into the DNA of proliferating cells to provide a marker for the DNA being replicated. The number of proliferating cells can then be detected in cell lysates, tissue sections or suspensions using an antibody specific for the BrdU.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Bromodeoxyuridine/BrdU Antibody (NB500-439)(9)

We have publications tested in 3 confirmed species: Human, Mouse, Non-species specific.

We have publications tested in 4 applications: ICC/IF, IF/IHC, IHC-Fr, IHC-P.


Filter By Application
ICC/IF
(3)
IF/IHC
(5)
IHC-Fr
(1)
IHC-P
(1)
All Applications
Filter By Species
Human
(1)
Mouse
(4)
Non-species specific
(1)
All Species
Showing Publications 1 - 9 of 9.
Publications using NB500-439 Applications Species
Castillo-Azofeifa D, Wald T, Reyes EA et al. A DLG1-ARHGAP31-CDC42 axis is essential for the intestinal stem cell response to fluctuating niche Wnt signaling Cell stem cell 2023-01-05 [PMID: 36640764] (IHC-Fr, IHC-P, Mouse) IHC-Fr, IHC-P Mouse
Abedin MR, Powers K, Aiardo R et al. Antibody-drug nanoparticle induces synergistic treatment efficacies in HER2 positive breast cancer cells Sci Rep 2021-04-02 [PMID: 33795712]

Details:
Citation using the IHC-Prediluted format of this antibody.
Xie MQ, Chen ZC, Zhang P et al. Newborn dopaminergic neurons are associated with the migration and differentiation of SVZ-derived neural progenitors in a 6-hydroxydopamin-injected mouse model. Neuroscience. 2017-04-04 [PMID: 28385636] (IF/IHC, Mouse) IF/IHC Mouse
Kollareddy M, Dimitrova E, Vallabhaneni KC et al. Regulation of nucleotide metabolism by mutant p53 contributes to its gain-of-function activities Nat Commun. 2015-06-13 [PMID: 26067754] (ICC/IF, Human) ICC/IF Human
Zhang P, Xie MQ, Ding YQ et al. Allopregnanolone enhances the neurogenesis of midbrain dopaminergic neurons in APPswe/PSEN1 mice Neuroscience. 2015-01-27 [PMID: 25637494] (IF/IHC, Mouse) IF/IHC Mouse
Adeosun SO, Hou X, Jiao Y et al. Source Allopregnanolone Reinstates Tyrosine Hydroxylase Immunoreactive Neurons and Motor Performance in an MPTP-Lesioned Mouse Model of Parkinson's Disease PLoS One 2012-01-01 [PMID: 23209637] (ICC/IF, IF/IHC) ICC/IF, IF/IHC
Hasegawa A, Liu H, Ling B et al. The level of monocyte turnover predicts disease progression in the macaque model of AIDS. Blood 2009-01-01 [PMID: 19383966]
Garcia L. Molecular dissection of prefrontal cortex development in health and disease: How serotonin makes up our mind Thesis 2019-07-09 (IF/IHC, Non-species specific) IF/IHC Non-species specific
Sun C, Ou X, Farley JM, Stockmeier C, Bigler S, Brinton RD, Wang JM. Allopregnanolone increases the number of dopaminergic neurons in substantia nigra of a triple transgenic mouse model of Alzheimer's disease. Curr Alzheimer Res;9(4):473-80. 2012-05-01 [PMID: 22272610] (IF/IHC, ICC/IF, Mouse) IF/IHC, ICC/IF Mouse

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

ICC/IF Video Protocol

FAQs for Bromodeoxyuridine/BrdU Antibody (NB500-439). (Showing 1 - 1 of 1 FAQs).

  1. I have been using your BrdU antibody for some time now and saw that you have discontinued it. I was wondering if you had any alternative antibodies that would also work? it is antibody NB500-169 lot# 1706-5
    • We recommend this product: NB500-235​

Secondary Antibodies

 

Isotype Controls

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The thymidine synthetic nucleoside analogue bromodeoxyuridine (BrdU) has a long, colorful history of repeated use in molecular and cytokinetic studies, as detailed in reviews by Vanderlaan and Dolbeare (1,2).  Because BrDU is only incorporated in...  Read full blog post.

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