Bromodeoxyuridine/BrdU Antibody (BRD494) [Alexa Fluor® 532]


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Product Details

Reactivity All-NASpecies Glossary
Applications Flow, ICC/IF, IHC, IHC-Fr, IHC-P
Alexa Fluor 532

Order Details

Bromodeoxyuridine/BrdU Antibody (BRD494) [Alexa Fluor® 532] Summary

Bromodeoxyuridine (BrdU) conjugated to KLH
It reacts with Bromodeoxyuridine (BrdU) in single stranded DNA (produced by partial denaturation of double stranded DNA), BrdU coupled to a protein carrier, as well as free BrdU. BrdU is a thymidine analog, incorporated into cell nuclei during DNA synthesis prior to mitosis. Antibody to BrdU is helpful in detecting S-phase cells, providing useful information on the aggressiveness of tumors.
Protein G purified
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  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Store at 4C in the dark.
50mM Sodium Borate
0.05% Sodium Azide
Protein G purified


Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Bromodeoxyuridine/BrdU Antibody (BRD494) [Alexa Fluor® 532]

  • 5-bromo-2-deoxyuridine
  • BrdU
  • Bromodeoxyuridine
  • BUdr


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

ICC/IF Video Protocol

FAQs for Bromodeoxyuridine/BrdU Antibody (NBP2-34566AF532). (Showing 1 - 1 of 1 FAQ).

  1. I have been using your BrdU antibody for some time now and saw that you have discontinued it. I was wondering if you had any alternative antibodies that would also work? it is antibody NB500-169 lot# 1706-5
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Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-34566AF350
Alexa Fluor 405 NBP2-34566AF405
Alexa Fluor 488 NBP2-34566AF488
Alexa Fluor 532 NBP2-34566AF532
Alexa Fluor 594 NBP2-34566AF594
Alexa Fluor 647 NBP2-34566AF647
Alexa Fluor 700 NBP2-34566AF700
Alexa Fluor 750 NBP2-34566AF750
Allophycocyanin NBP2-34566APC
Biotin NBP2-34566B
DyLight 350 NBP2-34566UV
DyLight 405 NBP2-34566V
DyLight 488 NBP2-34566G
DyLight 550 NBP2-34566R
DyLight 594 NBP2-34566DL594
DyLight 650 NBP2-34566C
DyLight 680 NBP2-34566FR
DyLight 755 NBP2-34566IR
FITC NBP2-34566F
HRP NBP2-34566H
Janelia Fluor 549 NBP2-34566JF549
Janelia Fluor 646 NBP2-34566JF646
PE NBP2-34566PE
PerCP NBP2-34566PCP

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BrDU (Bromodeoxyuridine)
The thymidine synthetic nucleoside analogue bromodeoxyuridine (BrdU) has a long, colorful history of repeated use in molecular and cytokinetic studies, as detailed in reviews by Vanderlaan and Dolbeare (1,2).  Because BrDU is only incorporated in...  Read full blog post.

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