Bromodeoxyuridine/BrdU Antibody (29G6.E8) - BSA Free

Western Blot: Bromodeoxyuridine/BrdU Antibody (29G6.E8) [NBP2-44055] - Lane 1: loading control. Lane 2: FdU. Lane 3: BrdU. Lane 4: IdU. Lane 5 CldU. Load: 20 Ug per lane.

Immunocytochemistry/Immunofluorescence: Bromodeoxyuridine/BrdU Antibody (29G6.E8) [NBP2-44055] - Tissue: OCT-embedded E10.5 mouse embryo. Localization: 20X, section through the developing hindbrain. Fixation: 4% PFA. Antigen retrieval: not required. Staining: Double labeled (green/blue) cells represent cells that were actively dividing.

Immunocytochemistry/Immunofluorescence: Bromodeoxyuridine/BrdU Antibody (29G6.E8) [NBP2-44055] - Tissue: OCT-embedded E10.5 mouse embryo. Localization: 20X, section through the developing limb bud. Fixation: 4% PFA. Antigen retrieval: not required.

Immunocytochemistry/Immunofluorescence: Bromodeoxyuridine/BrdU Antibody (29G6.E8) [NBP2-44055] - Tissue: OCT-embedded E10.5 mouse embryo. Localization: 40X, section through the developing limb bud. Fixation: 4% PFA. Antigen retrieval: not required. Primary antibody: BrdU antibody at 1:500 in 0.4% PBS+Triton with 1% normal sheep serum overnight at 4C. Staining: Double labeled (green/blue) cells represent cells that were actively dividing.

Bromodeoxyuridine/BrdU Antibody (29G6.E8) [NBP2-44055]

• Reactivity: All-NA
• Applications: WB, DB, ELISA, Flow, ICC/IF, IHC
• Clone: 29G6.E8
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

Bromodeoxyuridine/BrdU Antibody (29G6.E8) - BSA Free Summary

• Description: This antibody Monoclonal Antibody was purified from ascites fluid by Protein A chromatography
  
  Store this antibody at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
• Immunogen: Bromodeoxyuridine/Bromodeoxyuridine/BrdU antibody (29G6.E8) was produced in mice by repeated immunizations prepared via immunizations with BromodeoxyUridine-KLH followed by hybridoma development.
• Specificity: This antibody reacts strongly with Bromodeoxyuridine/BrdU. Cross-reactivity is observed with CldU and IdU.
• Isotype: IgG1 Lambda
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Dot Blot
  • ELISA 1:2000-1:10000
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:500-1:3000
  • Immunohistochemistry 1:100-1:500
  • Western Blot 1:2000-1:5000
• Application Notes: This product has been tested as suitable for immunofluorescence and immunoblot assays. Antibody may be suitable for additional immunoassays including flow cytometry and immunohistochemistry. Specific conditions for reactivity should be optimized by the end user. Antibody will detect incorporated BrdU thymidine analog from replicated cells.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Preservative: 0.01% Sodium Azide
• Purity: Protein A purified

Alternate Names for Bromodeoxyuridine/BrdU Antibody (29G6.E8) - BSA Free

• 5-bromo-2-deoxyuridine
• BrdU
• Bromodeoxyuridine
• BUdr

Background

Bromodeoxyuridine (BrdU) is a synthetic nucleoside analogue of thymidine which can be incorporated into DNA in proliferating cells. During mitosis, BrdU may be incorporated into the newly synthezised DNA of S-phase cells, and can therefore provide an estimate for the fraction of cells in S-phase. Labeling cells with BrdU and then screening with BrdU antibodies has become a powerful tool to study the cytokinetics of normal and neoplastic cells. In vivo, BrdU is considered a potential carcinogen, as its incorporation into DNA may cause mutations.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Bromodeoxyuridine/BrdU Antibody (NBP2-44055)(1)

Publication using NBP2-44055

• Lazarchuk P, Nguyen MM, Curca CM et Al. Werner syndrome RECQ helicase participates in and directs maintenance of the protein complexes of constitutive heterochromatin in proliferating human cells Aging (Albany NY) 2024-10-31 [PMID: 39422615]

FAQs for Bromodeoxyuridine/BrdU Antibody (NBP2-44055). (Showing 1 - 1 of 1 FAQ).

1. I have been using your BrdU antibody for some time now and saw that you have discontinued it. I was wondering if you had any alternative antibodies that would also work? it is antibody NB500-169 lot# 1706-5
   • We recommend this product: NB500-235​