Bromodeoxyuridine/BrdU Antibody (SPM166) [Alexa Fluor 647]

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Product Details

Summary
Reactivity AllSpecies Glossary
Applications Flow, ICC/IF, IHC-Fr, IHC-P
Clone
SPM166
Clonality
Monoclonal
Host
Mouse

Bromodeoxyuridine/BrdU Antibody (SPM166) [Alexa Fluor 647] Summary

Immunogen
Bromodeoxyuridine (BrdU) conjugated to KLH
Localization
Nuclear
Marker
Proliferation Marker
Specificity
It reacts with Bromodeoxyuridine (BrdU) in single stranded DNA (produced by partial denaturation of double stranded DNA), BrdU coupled to a protein carrier, as well as free BrdU. BrdU is a thymidine analog, incorporated into cell nuclei during DNA synthesis prior to mitosis. Antibody to BrdU is helpful in detecting S-phase cells, providing useful information on the aggressiveness of tumors.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Purity
Protein G purified
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Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Conjugate
Alexa Fluor 647
Format
Alexa Fluor 647 (A=650, E=668)
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
Application Notes
Immunohistochemistry (For staining of formalin-fixed tissues, incubate sections in 4N HCl for 30 minutes at RT followed by digestion with trypsin at 1mg/ml PBS, 10 min at 37C ]
Theoretical MW
307 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Alternate Names for Bromodeoxyuridine/BrdU Antibody (SPM166) [Alexa Fluor 647]

  • 5-bromo-2-deoxyuridine
  • BrdU
  • Bromodeoxyuridine
  • BUdr

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

ICC/IF Video Protocol

FAQs for Bromodeoxyuridine/BrdU Antibody (NBP2-34744AF647) (0)

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Secondary Antibodies

 

Isotype Controls

Additional Bromodeoxyuridine/BrdU Products

Bromodeoxyuridine/BrdU NBP2-34744AF647

Blogs on Bromodeoxyuridine/BrdU.

BrDU (Bromodeoxyuridine)
The thymidine synthetic nucleoside analogue bromodeoxyuridine (BrdU) has a long, colorful history of repeated use in molecular and cytokinetic studies, as detailed in reviews by Vanderlaan and Dolbeare (1,2).  Because BrDU is only incorporated in...  Read full blog post.

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