| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-22193-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-91660-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-54376-100ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47996-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-91659-25ul | |||
![Immunocytochemistry/Immunofluorescence: Alkaline Phosphatase, Tissue Non-Specific Antibody (SA40-00) [NBP2-67295] - Staining ALP in SW480 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Alkaline-Phosphatase-Tissue-Non-Specific-Antibody-SA40-00-Immunocytochemistry-Immunofluorescence-NBP2-67295-img0010.jpg "Immunocytochemistry/Immunofluorescence: Alkaline Phosphatase, Tissue Non-Specific Antibody (SA40-00) [NBP2-67295] - Staining ALP in SW480 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Flow Cytometry: Alkaline Phosphatase, Tissue Non-Specific Antibody (SA40-00) [NBP2-67295] - Analysis of Hela cells with ALP antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody](http://images.novusbio.com/fullsize/Alkaline-Phosphatase-Tissue-Non-Specific-Antibody-SA40-00-Flow-Cytometry-NBP2-67295-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Alkaline Phosphatase, Tissue Non-Specific Antibody (SA40-00) [NBP2-67295] - Staining ALP in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/Alkaline-Phosphatase-Tissue-Non-Specific-Antibody-SA40-00-Immunocytochemistry-Immunofluorescence-NBP2-67295-img0003.jpg)
![Immunohistochemistry-Paraffin: Alkaline Phosphatase, Tissue Non-Specific Antibody (SA40-00) [NBP2-67295] - Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Alkaline Phosphatase, Tissue Non-Specific antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The t](http://images.novusbio.com/fullsize/Alkaline-Phosphatase-Tissue-Non-Specific-Antibody-SA40-00-Immunohistochemistry-Paraffin-NBP2-67295-img0011.jpg)
![Immunohistochemistry-Paraffin: Alkaline Phosphatase, Tissue Non-Specific Antibody (SA40-00) [NBP2-67295] - Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue using anti-Alkaline Phosphatase, Tissue Non-Specific antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minut](http://images.novusbio.com/fullsize/Alkaline-Phosphatase-Tissue-Non-Specific-Antibody-SA40-00-Immunohistochemistry-Paraffin-NBP2-67295-img0012.jpg)
![Immunohistochemistry-Paraffin: Alkaline Phosphatase, Tissue Non-Specific Antibody (SA40-00) [NBP2-67295] - Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Alkaline Phosphatase, Tissue Non-Specific antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The t](http://images.novusbio.com/fullsize/Alkaline-Phosphatase-Tissue-Non-Specific-Antibody-SA40-00-Immunohistochemistry-Paraffin-NBP2-67295-img0013.jpg)
![Western Blot: Alkaline Phosphatase, Tissue Non-Specific Antibody (SA40-00) [NBP2-67295] -Analysis of Alkaline Phosphatase on different lysates with Rabbit anti-Alkaline Phosphatase antibody at 1/5,000 dilution.Lane 1: Saos-2 cell lysate (15ug/Lane)Lane 2: HeLa cell lysate (15 ug/Lane)Lane 3: A549 cell lysate (15 ug/Lane)Lane 4: Mouse liver tissue lysate (20 ug/Lane)Lane 5: Rat liver tissue lysate (20 ug/Lane)Predicted band size: 57 kDaObserved band size: 75 kDaExposure time: 24 seconds;4-20% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp2-67295_rabbit-monoclonal-alkaline-phosphatase-tissue-non-specific-antibody-sa40-00-western-blot-1482024111635..jpg)
![Western Blot: Alkaline Phosphatase, Tissue Non-Specific Antibody (TRA-2-49) [NB600-540] - Comparison on osteogenic-related proteins (OCN, BMP2, COL1a1, DDk1, ALP) for osteogenic micro-tissue and digested cells by Western blot. MT, osteogenic micro-tissue; DC, digested cells Image collected and cropped by CiteAb from the following publication (//stemcellres.biomedcentral.com/articles/10.1186/s13287-018-1064-1) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Alkaline-Phosphatase-Tissue-Non-Specific-Antibody-TRA-2-49-Western-Blot-NB600-540-img0009.jpg "Western Blot: Alkaline Phosphatase, Tissue Non-Specific Antibody (TRA-2-49) [NB600-540] - Comparison on osteogenic-related proteins (OCN, BMP2, COL1a1, DDk1, ALP) for osteogenic micro-tissue and digested cells by Western blot. MT, osteogenic micro-tissue; DC, digested cells Image collected and cropped by CiteAb from the following publication (//stemcellres.biomedcentral.com/articles/10.1186/s13287-018-1064-1) licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: Alkaline Phosphatase, Tissue Non-Specific Antibody (TRA-2-49) - BSA Free [NB600-540] - Confocal fluorescent analysis of A431 using anti-TRA-2-49 Mouse monoclonal antibody (Red). Actin filaments have been labeled with AlexaFluor(R) 488-Phalloidin (Green). Nucleus is stained with DAPI (Blue). Positive cytosol staining.](http://images.novusbio.com/fullsize/Alkaline-Phosphatase-Tissue-Non-Specific-Antibody-TRA-2-49-BSA-Free-Immunocytochemistry-Immunofluorescence-NB600-540-img0011.jpg)
![Flow Cytometry: Alkaline Phosphatase, Tissue Non-Specific Antibody (TRA-2-49) [NB600-540] - Analysis using anti-TRA-2-49. 2102Ep cells were stained with anti-TRA-2-49 antibody (Green) or isotype control antibody (grey).](http://images.novusbio.com/fullsize/Alkaline-Phosphatase-Tissue-Non-Specific-Antibody-TRA-2-49-Flow-Cytometry-NB600-540-img0008.jpg)
![Western Blot: Alkaline Phosphatase, Tissue Non-Specific Antibody (2F4) - BSA Free [NBP2-22193] - Analysis of Human engineered ALPL cells using Alkaline Phosphatase. Primary antibody dilution: 1:1000. Image from verified customer review.](http://images.novusbio.com/fullsize/Alkaline-Phosphatase-Tissue-Non-Specific-Antibody-2F4-BSA-Free-Western-Blot-NBP2-22193-img0007.jpg "Western Blot: Alkaline Phosphatase, Tissue Non-Specific Antibody (2F4) - BSA Free [NBP2-22193] - Analysis of Human engineered ALPL cells using Alkaline Phosphatase. Primary antibody dilution: 1:1000. Image from verified customer review.")
![Immunohistochemistry-Paraffin: Alkaline Phosphatase, Tissue Non-Specific Antibody (2F4) [NBP2-22193] - Analysis of ovarian cancer tissues using Alkaline Phosphatase, Tissue Non-Specific mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/Alkaline-Phosphatase-Tissue-Non-Specific-Antibody-2F4-Immunohistochemistry-Paraffin-NBP2-22193-img0002.jpg)
![Flow Cytometry: Alkaline Phosphatase, Tissue Non-Specific Antibody (2F4) - BSA Free [NBP2-22193] - Analysis of Human engineered ALPL cells using Alkaline Phosphatase. Image from verified customer reviews.](http://images.novusbio.com/fullsize/Alkaline-Phosphatase-Tissue-Non-Specific-Antibody-2F4-BSA-Free-Flow-Cytometry-NBP2-22193-img0006.jpg)
![Western Blot: Alkaline Phosphatase, Tissue Non-Specific Antibody (2F4) [NBP2-22193] - Analysis using Alkaline Phosphatase, Tissue Non-Specific mAb against human Alkaline Phosphatase, Tissue Non-Specific (AA: 18-502) recombinant protein. (Expected MW is 78.9 kDa)](http://images.novusbio.com/fullsize/Alkaline-Phosphatase-Tissue-Non-Specific-Antibody-2F4-Western-Blot-NBP2-22193-img0004.jpg)
![Western Blot: Alkaline Phosphatase, Tissue Non-Specific Antibody (2F4) [NBP2-22193] - Analysis using Alkaline Phosphatase, Tissue Non-Specific mouse mAb against HeLa (1), and NTERA-2 (4) cell lysate.](http://images.novusbio.com/fullsize/Alkaline-Phosphatase-Tissue-Non-Specific-Antibody-2F4-Western-Blot-NBP2-22193-img0005.jpg)
![Flow Cytometry: Alkaline Phosphatase, Tissue Non-Specific Antibody (2F4) [NBP2-22193] - Analysis of MCF-7 cells using Alkaline Phosphatase, Tissue Non-Specific mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize/Alkaline-Phosphatase-Tissue-Non-Specific-Antibody-2F4-Flow-Cytometry-NBP2-22193-img0001.jpg)
![ELISA: Alkaline Phosphatase, Tissue Non-Specific Antibody (2F4) [NBP2-22193] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng)](http://images.novusbio.com/fullsize/Alkaline-Phosphatase-Tissue-Non-Specific-Antibody-2F4-ELISA-NBP2-22193-img0003.jpg)
![Immunohistochemistry-Paraffin: Alkaline Phosphatase, Tissue Non-Specific Antibody [NBP1-91660] - Staining of human adrenal gland, endometrium, liver and pancreas using Anti-Alkaline Phosphatase, Tissue Non-Specific antibody NBP1-91660 (A) shows similar protein distribution across tissues to independent antibody NBP1-91659 (B).](http://images.novusbio.com/fullsize/Alkaline-Phosphatase--Tissue-Non-Specific-Antibody-Immunohistochemistry-Paraffin-NBP1-91660-img0012.jpg "<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: Alkaline Phosphatase, Tissue Non-Specific Antibody [NBP1-91660] - Staining of human adrenal gland, endometrium, liver and pancreas using Anti-Alkaline Phosphatase, Tissue Non-Specific antibody NBP1-91660 (A) shows similar protein distribution across tissues to independent antibody NBP1-91659 (B).")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: Alkaline Phosphatase, Tissue Non-Specific Antibody [NBP1-91660] - Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.](http://images.novusbio.com/fullsize/Alkaline-Phosphatase--Tissue-Non-Specific-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-91660-img0004.jpg)
![Immunohistochemistry-Paraffin: Alkaline Phosphatase, Tissue Non-Specific Antibody [NBP1-91660] - Staining of human pancreas shows no postivity in glandular cells as expected.](http://images.novusbio.com/fullsize/Alkaline-Phosphatase--Tissue-Non-Specific-Antibody-Immunohistochemistry-Paraffin-NBP1-91660-img0015.jpg)
![Immunohistochemistry-Paraffin: Alkaline Phosphatase, Tissue Non-Specific Antibody [NBP1-91660] - Staining of human adrenal gland shows strong membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/Alkaline-Phosphatase--Tissue-Non-Specific-Antibody-Immunohistochemistry-Paraffin-NBP1-91660-img0010.jpg)
![Immunohistochemistry-Paraffin: Alkaline Phosphatase, Tissue Non-Specific Antibody [NBP1-91660] - Staining of human endometrium shows strong positivity in apical membrane in glandular cells.](http://images.novusbio.com/fullsize/Alkaline-Phosphatase--Tissue-Non-Specific-Antibody-Immunohistochemistry-Paraffin-NBP1-91660-img0013.jpg)
![Immunohistochemistry-Paraffin: Alkaline Phosphatase, Tissue Non-Specific Antibody [NBP1-91660] - Staining of human liver shows moderate membranous positivity in hepatocytes.](http://images.novusbio.com/fullsize/Alkaline-Phosphatase--Tissue-Non-Specific-Antibody-Immunohistochemistry-Paraffin-NBP1-91660-img0014.jpg)
![Western Blot: Alkaline Phosphatase, Tissue Non-Specific Antibody [NBP1-32948] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Alkaline Phosphatase, Tissue Non-Specific antibody (NBP1-32948) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-32948_rabbit-polyclonal-alkaline-phosphatase-tissue-non-specific-antibody-910202420134024.jpg "Western Blot: Alkaline Phosphatase, Tissue Non-Specific Antibody [NBP1-32948] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Alkaline Phosphatase, Tissue Non-Specific antibody (NBP1-32948) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Alkaline Phosphatase, Tissue Non-Specific Antibody [NBP1-32948] - Deprivation of NO induces bi-lineage phenotype and expression of stem cell markers in mammary epithelial cells both in vivo and in 3D cultures. Representative images of drug-treated mammary glands stained for stem cell markers: alkaline phosphatase (AP) (top 2 rows), CD44 (middle 2 rows) or CD24 (bottom 2 rows). Nuclei were counterstained with DAPI (blue). Scale bars: 50 um. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-019-43239-x) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Alkaline-Phosphatase--Tissue-Non-Specific-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32948-img0006.jpg)
![Immunohistochemistry-Paraffin: Alkaline Phosphatase, Tissue Non-Specific Antibody [NBP1-32948] - Paraffin-embedded OVCAR3 xenograft, using antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/Alkaline-Phosphatase-Tissue-Non-Specific-Antibody-Immunohistochemistry-Paraffin-NBP1-32948-img0003.jpg)
![Western Blot: Alkaline Phosphatase, Tissue Non-Specific Antibody (S08-6H0) [NBP3-19565] - Western blot detection of Alkaline Phosphatase, Tissue Non-Specific in Hela, C6 cell lysates using NBP3-19565 (1:500 diluted). Predicted band size: 57kDa. Observed band size: 80kDa.](http://images.novusbio.com/fullsize/Alkaline-Phosphatase-Tissue-Non-Specific-Antibody-S08-6H0-Western-Blot-NBP3-19565-img0003.jpg "Western Blot: Alkaline Phosphatase, Tissue Non-Specific Antibody (S08-6H0) [NBP3-19565] - Western blot detection of Alkaline Phosphatase, Tissue Non-Specific in Hela, C6 cell lysates using NBP3-19565 (1:500 diluted). Predicted band size: 57kDa. Observed band size: 80kDa.")
![Immunocytochemistry/Immunofluorescence: Alkaline Phosphatase, Tissue Non-Specific Antibody (S08-6H0) [NBP3-19565] - Immunofluorescence of Alkaline Phosphatase, Tissue Non-Specific (green) in Hela using NBP3-19565 at dilution 1/5, and DAPI (blue)](http://images.novusbio.com/fullsize/Alkaline-Phosphatase-Tissue-Non-Specific-Antibody-S08-6H0-Immunocytochemistry-Immunofluorescence-NBP3-19565-img0001.jpg)
![Immunohistochemistry-Paraffin: Alkaline Phosphatase, Tissue Non-Specific Antibody (S08-6H0) [NBP3-19565] - Immunohistochemistry of Alkaline Phosphatase, Tissue Non-Specific in paraffin-embedded Human lung cancer tissue using NBP3-19565 at dilution 1/50](http://images.novusbio.com/fullsize/Alkaline-Phosphatase-Tissue-Non-Specific-Antibody-S08-6H0-Immunohistochemistry-Paraffin-NBP3-19565-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Alkaline Phosphatase, Tissue Non-Specific Antibody (S08-6H0) [NBP3-19565] - Immunofluorescence of Alkaline Phosphatase, Tissue Non-Specific (green) in Hela using NBP3-19565 at dilution 1/5, and DAPI (blue)](http://images.novusbio.com/fullsize/nbp3-19565_rb-alkaline-phosphatase-tissue-non-specific-mab-s08-6h0-11320251555912.jpg)
![Immunohistochemistry-Paraffin: Alkaline Phosphatase, Tissue Non-Specific Antibody (ALPL/597) - Azide and BSA Free [NBP2-54376] - Formalin-fixed, paraffin-embedded human Ovarian Carcinoma stained with Alkaline Phosphatase Mouse Monoclonal Antibody (ALPL/597).](http://images.novusbio.com/fullsize/Alkaline-Phosphatase-Tissue-Non-Specific-Antibody-ALPL-597-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-54376-img0008.jpg "Immunohistochemistry-Paraffin: Alkaline Phosphatase, Tissue Non-Specific Antibody (ALPL/597) - Azide and BSA Free [NBP2-54376] - Formalin-fixed, paraffin-embedded human Ovarian Carcinoma stained with Alkaline Phosphatase Mouse Monoclonal Antibody (ALPL/597).")
![Immunohistochemistry-Paraffin: Alkaline Phosphatase, Tissue Non-Specific Antibody (ALPL/597) - Azide and BSA Free [NBP2-54376] - Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Alkaline Phosphatase Mouse Monoclonal Antibody (ALPL/597).](http://images.novusbio.com/fullsize/Alkaline-Phosphatase-Tissue-Non-Specific-Antibody-ALPL-597-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-54376-img0005.jpg)
![Immunohistochemistry-Paraffin: Alkaline Phosphatase, Tissue Non-Specific Antibody (ALPL/597) - Azide and BSA Free [NBP2-54376] - Formalin-fixed, paraffin-embedded human Endometrial Carcinoma stained with Alkaline Phosphatase Mouse Monoclonal Antibody (ALPL/597).](http://images.novusbio.com/fullsize/Alkaline-Phosphatase-Tissue-Non-Specific-Antibody-ALPL-597-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-54376-img0006.jpg)
![Immunohistochemistry-Paraffin: Alkaline Phosphatase, Tissue Non-Specific Antibody (ALPL/597) - Azide and BSA Free [NBP2-54376] - Formalin-fixed, paraffin-embedded human Testicular Carcinoma stained with Alkaline Phosphatase Mouse Monoclonal Antibody (ALPL/597).](http://images.novusbio.com/fullsize/Alkaline-Phosphatase-Tissue-Non-Specific-Antibody-ALPL-597-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-54376-img0007.jpg)
![Immunohistochemistry-Paraffin: Alkaline Phosphatase, Tissue Non-Specific Antibody (ALPL/597) [NBP2-53240] - Formalin-fixed, paraffin-embedded human Ovarian Carcinoma stained with Alkaline Phosphatase Mouse Monoclonal Antibody (ALPL/597).](http://images.novusbio.com/fullsize/Alkaline-Phosphatase-Tissue-Non-Specific-Antibody-ALPL-597-Immunohistochemistry-Paraffin-NBP2-53240-img0008.jpg "Immunohistochemistry-Paraffin: Alkaline Phosphatase, Tissue Non-Specific Antibody (ALPL/597) [NBP2-53240] - Formalin-fixed, paraffin-embedded human Ovarian Carcinoma stained with Alkaline Phosphatase Mouse Monoclonal Antibody (ALPL/597).")
![Immunohistochemistry-Paraffin: Alkaline Phosphatase, Tissue Non-Specific Antibody (ALPL/597) [NBP2-53240] - Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Alkaline Phosphatase Mouse Monoclonal Antibody (ALPL/597).](http://images.novusbio.com/fullsize/Alkaline-Phosphatase-Tissue-Non-Specific-Antibody-ALPL-597-Immunohistochemistry-Paraffin-NBP2-53240-img0005.jpg)
![Immunohistochemistry-Paraffin: Alkaline Phosphatase, Tissue Non-Specific Antibody (ALPL/597) [NBP2-53240] - Formalin-fixed, paraffin-embedded human Endometrial Carcinoma stained with Alkaline Phosphatase Mouse Monoclonal Antibody (ALPL/597).](http://images.novusbio.com/fullsize/Alkaline-Phosphatase-Tissue-Non-Specific-Antibody-ALPL-597-Immunohistochemistry-Paraffin-NBP2-53240-img0006.jpg)
![Immunohistochemistry-Paraffin: Alkaline Phosphatase, Tissue Non-Specific Antibody (ALPL/597) [NBP2-53240] - Formalin-fixed, paraffin-embedded human Testicular Carcinoma stained with Alkaline Phosphatase Mouse Monoclonal Antibody (ALPL/597).](http://images.novusbio.com/fullsize/Alkaline-Phosphatase-Tissue-Non-Specific-Antibody-ALPL-597-Immunohistochemistry-Paraffin-NBP2-53240-img0007.jpg)
![Immunohistochemistry-Paraffin: Alkaline Phosphatase, Tissue Non-Specific Antibody (V17.1) [NBP2-44962] - Formalin-fixed, paraffin-embedded human Ovarian Carcinoma stained with Alkaline Phosphatase, Tissue Non-Specific Antibody (V17.1).](http://images.novusbio.com/fullsize/Alkaline-Phosphatase-Tissue-Non-Specific-Antibody-V17-1-Immunohistochemistry-Paraffin-NBP2-44962-img0002.jpg "Immunohistochemistry-Paraffin: Alkaline Phosphatase, Tissue Non-Specific Antibody (V17.1) [NBP2-44962] - Formalin-fixed, paraffin-embedded human Ovarian Carcinoma stained with Alkaline Phosphatase, Tissue Non-Specific Antibody (V17.1).")
![Immunohistochemistry-Paraffin: Alkaline Phosphatase, Tissue Non-Specific Antibody [NBP1-91659] - Staining of human adrenal gland, endometrium, liver and pancreas using Anti-Alkaline Phosphatase, Tissue Non-Specific antibody NBP1-91659 (A) shows similar protein distribution across tissues to independent antibody NBP1-91660 (B).](http://images.novusbio.com/fullsize/Alkaline-Phosphatase--Tissue-Non-Specific-Antibody-Immunohistochemistry-Paraffin-NBP1-91659-img0012.jpg "<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: Alkaline Phosphatase, Tissue Non-Specific Antibody [NBP1-91659] - Staining of human adrenal gland, endometrium, liver and pancreas using Anti-Alkaline Phosphatase, Tissue Non-Specific antibody NBP1-91659 (A) shows similar protein distribution across tissues to independent antibody NBP1-91660 (B).")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: Alkaline Phosphatase, Tissue Non-Specific Antibody [NBP1-91659] - Staining of human pancreas shows no positivity in glandular cells as expected.](http://images.novusbio.com/fullsize/Alkaline-Phosphatase--Tissue-Non-Specific-Antibody-Immunohistochemistry-Paraffin-NBP1-91659-img0015.jpg)
![Immunohistochemistry-Paraffin: Alkaline Phosphatase, Tissue Non-Specific Antibody [NBP1-91659] - Staining of human adrenal gland shows strong membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/Alkaline-Phosphatase--Tissue-Non-Specific-Antibody-Immunohistochemistry-Paraffin-NBP1-91659-img0010.jpg)
![Immunohistochemistry-Paraffin: Alkaline Phosphatase, Tissue Non-Specific Antibody [NBP1-91659] - Staining of human endometrium shows strong positivity in apical membrane in glandular cells.](http://images.novusbio.com/fullsize/Alkaline-Phosphatase--Tissue-Non-Specific-Antibody-Immunohistochemistry-Paraffin-NBP1-91659-img0013.jpg)
![Immunohistochemistry-Paraffin: Alkaline Phosphatase, Tissue Non-Specific Antibody [NBP1-91659] - Staining of human liver shows weak membranous positivity in hepatocytes.](http://images.novusbio.com/fullsize/Alkaline-Phosphatase--Tissue-Non-Specific-Antibody-Immunohistochemistry-Paraffin-NBP1-91659-img0014.jpg)
![Western Blot: Alkaline Phosphatase, Tissue Non-Specific Antibody (4H1) [H00000249-M01] - Analysis of ALPL expression in transfected 293T cell line by ALPL monoclonal antibody (M01), clone 4H1.Lane 1: ALPL transfected lysate(57.3 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Alkaline-Phosphatase-Tissue-Non-Specific-Antibody-4H1-Western-Blot-H00000249-M01-img0007.jpg "Western Blot: Alkaline Phosphatase, Tissue Non-Specific Antibody (4H1) [H00000249-M01] - Analysis of ALPL expression in transfected 293T cell line by ALPL monoclonal antibody (M01), clone 4H1.Lane 1: ALPL transfected lysate(57.3 KDa).Lane 2: Non-transfected lysate.")
![Western Blot: Alkaline Phosphatase, Tissue Non-Specific Antibody (4H1) [H00000249-M01] - ALPL monoclonal antibody (M01), clone 4H1 Western Blot analysis of ALPL expression in HeLa ( Cat # L013V1 ).](http://images.novusbio.com/fullsize/Alkaline-Phosphatase-Tissue-Non-Specific-Antibody-4H1-Western-Blot-H00000249-M01-img0001.jpg)
![Western Blot: Alkaline Phosphatase, Tissue Non-Specific Antibody (4H1) [H00000249-M01] - Analysis of ALPL over-expressed 293 cell line, cotransfected with ALPL Validated Chimera RNAi ( Cat # H00000249-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with ALPL monoclonal antibody (M01), clone 4H1 (Cat # H00000249-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.](http://images.novusbio.com/fullsize/Alkaline-Phosphatase-Tissue-Non-Specific-Antibody-4H1-Western-Blot-H00000249-M01-img0005.jpg)
![ELISA: Alkaline Phosphatase, Tissue Non-Specific Antibody (4H1) [H00000249-M01] - Detection limit for recombinant GST tagged ALPL is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Alkaline-Phosphatase-Tissue-Non-Specific-Antibody-4H1-ELISA-H00000249-M01-img0006.jpg)
![Western Blot: Alkaline Phosphatase, Tissue Non-Specific Antibody (HL2003) - Azide and BSA Free [NBP3-25356] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Alkaline Phosphatase (Tissue Non-Specific) antibody [HL2003] (NBP3-25356) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25356_rabbit-alkaline-phosphatase-tissue-non-specific-mab-hl2003-azide-bsa-free-2120249374918.jpg "Western Blot: Alkaline Phosphatase, Tissue Non-Specific Antibody (HL2003) - Azide and BSA Free [NBP3-25356] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Alkaline Phosphatase (Tissue Non-Specific) antibody [HL2003] (NBP3-25356) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Western Blot: Alkaline Phosphatase, Tissue Non-Specific Antibody (HL2003) - Azide and BSA Free [NBP3-25356] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Alkaline Phosphatase (Tissue Non-Specific) antibody [HL2003] (NBP3-25356) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp3-25356_rabbit-alkaline-phosphatase-tissue-non-specific-mab-hl2003-azide-bsa-free-2120249383191.jpg)
![Western Blot: Alkaline Phosphatase, Tissue Non-Specific Antibody (HL2003) - Azide and BSA Free [NBP3-25356] - Whole cell extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with Alkaline Phosphatase (Tissue Non-Specific) antibody [HL2003] (NBP3-25356) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25356_rabbit-alkaline-phosphatase-tissue-non-specific-mab-hl2003-azide-bsa-free-212024940384.jpg)
![Western Blot: Alkaline Phosphatase, Tissue Non-Specific Antibody (HL2003) - Azide and BSA Free [NBP3-25356] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Alkaline Phosphatase (Tissue Non-Specific) antibody [HL2003] (NBP3-25356) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp3-25356_rabbit-alkaline-phosphatase-tissue-non-specific-mab-hl2003-azide-bsa-free-2120249393565.jpg)