ACE-2 Antibodies

HAF008). A specific band was detected for ACE‑2 at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_lightbox"> HAF008). A specific band was detected for ACE‑2 at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." alt="Western blot shows lysates of mouse kidney, mouse ovary, mouse heart, and mouse testis. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Mouse ACE‑2 Monoclonal Antibody (Catalog # MAB34372) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (HAF008). A specific band was detected for ACE‑2 at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_thumb" />

F0111). Quadrant markers were set based on Rabbit IgG Isotype Control (MAB1050). Staining was performed using our Staining Membrane-associated Proteins protocol. " class="big_lightbox"> F0111). Quadrant markers were set based on Rabbit IgG Isotype Control (MAB1050). Staining was performed using our Staining Membrane-associated Proteins protocol. " class="big_thumb" />

NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox"> NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

HAF008). A specific band was detected for ACE‑2 at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_lightbox"> HAF008). A specific band was detected for ACE‑2 at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." alt="Western blot shows lysates of human kidney and human testis. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human ACE‑2 Monoclonal Antibody (Catalog # MAB10823) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (HAF008). A specific band was detected for ACE‑2 at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_thumb" />

F0111). Quadrant markers were set based on Rabbit IgG Isotype Control (MAB1050). Staining was performed using our Staining Membrane-associated Proteins protocol." class="big_lightbox"> F0111). Quadrant markers were set based on Rabbit IgG Isotype Control (MAB1050). Staining was performed using our Staining Membrane-associated Proteins protocol." class="big_thumb" />

VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane in convoluted tubules. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox"> VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane in convoluted tubules. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />

NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox"> NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells." alt="ACE‑2 was detected in immersion fixed HepG2 human hepatocellular carcinoma cell (positive staining) line and MCF‑7 human breast cancer cell line (negative control) using Mouse Anti-Human ACE‑2 Monoclonal Antibody (Catalog # MAB9335) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell membrane in convoluted tubules. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox"> VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell membrane in convoluted tubules. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />

HAF018). A specific band was detected for ACE‑2 at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_lightbox"> HAF018). A specific band was detected for ACE‑2 at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." alt="Western blot shows lysates of human testis. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human ACE‑2 Monoclonal Antibody (Catalog # MAB10826) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for ACE‑2 at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_thumb" />

VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell membranes in convoluted tubules. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox"> VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell membranes in convoluted tubules. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />

HAF008). A specific band was detected for ACE‑2 at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_lightbox"> HAF008). A specific band was detected for ACE‑2 at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." alt="Western blot shows lysates of human kidney, human heart, and human ovary. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human ACE‑2 Monoclonal Antibody (Catalog # MAB9337) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (HAF008). A specific band was detected for ACE‑2 at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_thumb" />

HAF018). A specific band was detected for ACE‑2 at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_lightbox"> HAF018). A specific band was detected for ACE‑2 at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." alt="Western blot shows lysates of human kidney. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human ACE‑2 Monoclonal Antibody (Catalog # MAB10821) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for ACE‑2 at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_thumb" />

MAB0061) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (F0113) and Mouse anti-Human CD14 Phycoerythrin-conjugated Monoclonal Antibody (FAB3832P). Staining was performed using our Staining Membrane-associated Proteins protocol. " class="big_lightbox"> MAB0061) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (F0113) and Mouse anti-Human CD14 Phycoerythrin-conjugated Monoclonal Antibody (FAB3832P). Staining was performed using our Staining Membrane-associated Proteins protocol. " alt="Human PBMC were stained with (A) Rat Anti-Human ACE-2 Monoclonal Antibody (Catalog # MAB9334) or (B) Rat IgG2b Isotype Control Antibody (MAB0061) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (F0113) and Mouse anti-Human CD14 Phycoerythrin-conjugated Monoclonal Antibody (FAB3832P). Staining was performed using our Staining Membrane-associated Proteins protocol. " class="big_thumb" />

DY990). Goat Anti-Mouse ACE‑2 Antigen Affinity-purified Polyclonal Antibody (AF3437) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994)." class="big_lightbox"> DY990). Goat Anti-Mouse ACE‑2 Antigen Affinity-purified Polyclonal Antibody (AF3437) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994)." alt="Recombinant Mouse ACE‑2 protein was serially diluted 2-fold and captured by Rat Anti-Mouse ACE‑2 Monoclonal Antibody (Catalog # MAB34371) coated on a Clear Polystyrene Microplate (DY990). Goat Anti-Mouse ACE‑2 Antigen Affinity-purified Polyclonal Antibody (AF3437) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994)." class="big_thumb" />

3437-ZN) was serially diluted and captured by Rat Anti-Mouse ACE‑2 Monoclonal Antibody (Catalog # MAB34371) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse ACE‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3437) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 3437-ZN) was serially diluted and captured by Rat Anti-Mouse ACE‑2 Monoclonal Antibody (Catalog # MAB34371) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse ACE‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3437) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />