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HAF017). A specific band was detected for P-Selectin/CD62P at approximately 140-150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox" target="_blank">
VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to endothelial cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox" target="_blank">
HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_lightbox" target="_blank">
737-PS) was serially diluted and captured by Rat Anti-Mouse P‑Selectin/CD62P Monoclonal Antibody (Catalog # MAB737) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse/Rat P‑Selectin/CD62P Antigen Affinity-purified Polyclonal Antibody (Catalog # AF737) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox" target="_blank">
HAF016). A specific band was detected for P-Selectin/ CD62P at approximately 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox" target="_blank">
HAF016). A specific band was detected for P-Selectin/CD62P at approximately 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox" target="_blank">
HAF016). This experiment was conducted under reducing conditions and using the 66-440 kDa separation system." class="big_lightbox" target="_blank">
CD62P is a member of the small selectin family of cellular adhesion molecules, which also includes CD62E and CD62L. Its structure, similar to the other members of the selectin family, consists of an N terminal lectin like domain of C type, followed by an epidermal growth factor like motif, a series of short consensus repeats, a transmembrane domain, and a cytoplasmic tail. The CD62P antigen is a 140 kDa glycoprotein, located in the alpha granules and the dense granules of platelets and endothelial cells. Activation of these cells results in rapid mobilization of CD62P from the storage granules to the cell surface. Activated platelets have a stable CD62P expression, while the endothelial cells lose CD62P expression within 1 h of activation, because of endocytosis of the molecule. CD62P is also expressed on megakaryocytes, but resting platelets and endothelial cells show no surface staining of CD62P.
![Western Blot P-Selectin/CD62P Antibody [Unconjugated]](http://images.novusbio.com/images/antibody/PSelectin_AF737_Western_Blot_21309.jpg)
![Western Blot P-Selectin/CD62P Antibody [Unconjugated]](http://images.novusbio.com/images/antibody/PSelectin_AF137_Western_Blot_20033.jpg)
![N/A P-Selectin/CD62P [Biotin]](http://images.novusbio.com/fullsize/elisa/DATA_PSelectin_DY137_ELISA_1636.jpg)
![N/A P-Selectin/CD62P [HRP]](http://images.novusbio.com/fullsize/elisa/DATA_PSelectin_MPS00_ELISA_1017.jpg)
![N/A P-Selectin/CD62P [HRP]](http://images.novusbio.com/fullsize/elisa/PSelectin_DPSE00_ELISA_2643.jpg)
![Immunohistochemistry P-Selectin/CD62P Antibody [Unconjugated]](http://images.novusbio.com/images/antibody/PSelectin_AF737_Immunohistochemistry_22267.jpg)
![Simple Western P-Selectin/CD62P Antibody [Unconjugated]](http://images.novusbio.com/images/antibody/PSelectin_AF737_Simple_Western_21453.jpg)
![Immunocytochemistry/ Immunofluorescence P-Selectin/CD62P Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af737_mouse-rat-p-selectin-cd62p-affinity-purified-polyclonal-ab-8220249503262.jpg)
![Immunocytochemistry/ Immunofluorescence P-Selectin/CD62P Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af737_mouse-rat-p-selectin-cd62p-affinity-purified-polyclonal-ab-8220249503234.jpg)
![Immunocytochemistry/ Immunofluorescence P-Selectin/CD62P Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af737_mouse-rat-p-selectin-cd62p-affinity-purified-polyclonal-ab-44202415425067.jpg)
![Immunohistochemistry P-Selectin/CD62P Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af737_mouse-rat-p-selectin-cd62p-affinity-purified-polyclonal-ab-immunohistochemistry-1212202596168.jpg)
![Immunohistochemistry P-Selectin/CD62P Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af737_mouse-rat-p-selectin-cd62p-affinity-purified-polyclonal-ab-immunohistochemistry-121220259211116.jpg)
![ELISA P-Selectin/CD62P Antibody [Unconjugated]](http://images.novusbio.com/images/af737_mouse-rat-p-selectin-cd62p-affinity-purified-polyclonal-ab-elisa-639201854772674465.jpg)
![Western Blot P-Selectin/CD62P Antibody [Unconjugated]](http://images.novusbio.com/images/antibody/PSelectin_AF137_Western_Blot_21319.jpg)
![Simple Western P-Selectin/CD62P Antibody [Unconjugated]](http://images.novusbio.com/images/antibody/af137_human-rat-p-selectin-cd62p-affinity-purified-polyclonal-ab-simple-western-1102021142554.jpg)
![N/A P-Selectin/CD62P [HRP]](http://images.novusbio.com/fullsize/elisa/P-Selectin_MPS00_ELISA_309.jpg)
![N/A P-Selectin/CD62P [HRP]](http://images.novusbio.com/fullsize/elisa/PSelectin_DPSE00_ELISA_2644.jpg)
