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![Western Blot: CDC73/HRPT2 Antibody [NB200-184] - Detection of Human and Mouse Para fibromin by Western Blot. Samples: Whole cell lysate (50 ug) from mouse TCMK-1 and human 293T cells. Antibodies: Affinity purified rabbit anti-Parafibromin antibody NB200-184 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize2/CDC73-HRPT2-Antibody-Western-Blot-NB200-184-img0006.jpg "<b>Biological Strategies Validation. </b>Western Blot: CDC73/HRPT2 Antibody [NB200-184] - Detection of Human and Mouse Para fibromin by Western Blot. Samples: Whole cell lysate (50 ug) from mouse TCMK-1 and human 293T cells. Antibodies: Affinity purified rabbit anti-Parafibromin antibody NB200-184 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Biological Strategies Validation. </b>Immunoprecipitation: CDC73/HRPT2 Antibody [NB200-184] - Detection of human CDC73/HRPT2 (Parafibromin) by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HEK293T cells. Antibodies: Affinity purified rabbit anti-CDC73/HRPT2 antibody NB200-184 used for IP at 6 ug/mg lysate. For blotting immunoprecipitated CDC73/HRPT2, NB200-184 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize2/CDC73-HRPT2-Antibody-Immunoprecipitation-NB200-184-img0007.jpg)
![Western Blot: CDC73/HRPT2 Antibody [NBP2-59257] - Western blot was performed on whole cell extracts (WCE) from drosophila larva and drosophila adults (Figure A) or on nuclear extracts from HeLa cells (HeLa NE, 20 ug) (Figure B) using the antibody against dCdc73 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. A molecular weight marker (kDa) is shown on the left; the location of the protein of interest is indicated on the right.](http://images.novusbio.com/fullsize2/CDC73-HRPT2-Antibody-Western-Blot-NBP2-59257-img0001.jpg "Western Blot: CDC73/HRPT2 Antibody [NBP2-59257] - Western blot was performed on whole cell extracts (WCE) from drosophila larva and drosophila adults (Figure A) or on nuclear extracts from HeLa cells (HeLa NE, 20 ug) (Figure B) using the antibody against dCdc73 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. A molecular weight marker (kDa) is shown on the left; the location of the protein of interest is indicated on the right.")
![Western Blot: CDC73/HRPT2 Antibody [NBP1-32972] - Sample (30 ug of whole cell lysate) A: Molt-4 7. 5% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize2/CDC73-HRPT2-Antibody-Western-Blot-NBP1-32972-img0004.jpg "Western Blot: CDC73/HRPT2 Antibody [NBP1-32972] - Sample (30 ug of whole cell lysate) A: Molt-4 7. 5% SDS PAGE; antibody diluted at 1:1000.")
![Immunocytochemistry/Immunofluorescence: CDC73/HRPT2 Antibody [NBP3-17817] - Staining of human cell line U-2 OS shows localization to nucleoplasm.](http://images.novusbio.com/fullsize2/CDC73-HRPT2-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-17817-img0001.jpg "Immunocytochemistry/Immunofluorescence: CDC73/HRPT2 Antibody [NBP3-17817] - Staining of human cell line U-2 OS shows localization to nucleoplasm.")
![Immunocytochemistry/Immunofluorescence: CDC73/HRPT2 Antibody [NBP2-55257] - Staining of human cell line MCF7 shows localization to nucleus. Antibody staining is shown in green.](http://images.novusbio.com/fullsize2/CDC73-HRPT2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-55257-img0001.jpg "Immunocytochemistry/Immunofluorescence: CDC73/HRPT2 Antibody [NBP2-55257] - Staining of human cell line MCF7 shows localization to nucleus. Antibody staining is shown in green.")
![Western Blot: HRPT2 Overexpression Lysate (Adult Normal) [NBL1-09015] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for HRPT2.](http://images.novusbio.com/fullsize/HRPT2-Overexpression-Lysate-(Adult-Normal)-Western-Blot-NBL1-09015-img0002.jpg "Western Blot: HRPT2 Overexpression Lysate (Adult Normal) [NBL1-09015] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for HRPT2.")