Timp1 Products By Gene Name

HAF017). A specific band was detected for TIMP-1 at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF017). A specific band was detected for TIMP-1 at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of human lung tissue and human prostate tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human TIMP-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF970) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for TIMP-1 at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

HAF017). A specific band was detected for TIMP-1 at approximately 26 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF017). A specific band was detected for TIMP-1 at approximately 26 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and extracellular space. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox"> VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and extracellular space. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />

HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_lightbox"> HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_thumb" />

HAF017). A specific band was detected for TIMP-1 at approximately 25 kDa (as indicated) in the parental SK-OV-3 cell line, but is not detectable in knockout SK-OV-3 cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF017). A specific band was detected for TIMP-1 at approximately 25 kDa (as indicated) in the parental SK-OV-3 cell line, but is not detectable in knockout SK-OV-3 cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

902-MP) activity is measured in the presence of Recombinant Human TIMP-1 (0.1 µg/mL, Catalog # 970-TM) that has been preincubated with increasing concentrations of Goat Anti-Human TIMP-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF970). The ND₅₀ is typically 1 µg/mL." class="big_lightbox"> 902-MP) activity is measured in the presence of Recombinant Human TIMP-1 (0.1 µg/mL, Catalog # 970-TM) that has been preincubated with increasing concentrations of Goat Anti-Human TIMP-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF970). The ND₅₀ is typically 1 µg/mL." class="big_thumb" />

970-TM) was serially diluted and captured by Mouse Anti-Human TIMP‑1 Monoclonal Antibody (Catalog # MAB970) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human TIMP‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF970) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 970-TM) was serially diluted and captured by Mouse Anti-Human TIMP‑1 Monoclonal Antibody (Catalog # MAB970) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human TIMP‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF970) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

980-MT) was serially diluted and captured by Rat Anti-Mouse TIMP‑1 Monoclonal Antibody (Catalog # MAB980) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse TIMP‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF980) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 980-MT) was serially diluted and captured by Rat Anti-Mouse TIMP‑1 Monoclonal Antibody (Catalog # MAB980) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse TIMP‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF980) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and endoplasmic reticulum. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox"> NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and endoplasmic reticulum. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." alt="TIMP-1 was detected in immersion fixed Wi-38 human lung fibroblast cell line using Mouse Anti-Human TIMP-1 Monoclonal Antibody (Catalog # MAB970) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and endoplasmic reticulum. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox"> CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

970-TM) was serially diluted and captured by Mouse Anti-Human TIMP‑1 Monoclonal Antibody (Catalog # MAB970) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human TIMP‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF970) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 970-TM) was serially diluted and captured by Mouse Anti-Human TIMP‑1 Monoclonal Antibody (Catalog # MAB970) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human TIMP‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF970) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox"> VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." alt="TIMP‑1 was detected in perfusion fixed paraffin-embedded sections of Rat Brain using Goat Anti-Rat TIMP‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF580) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />

580-RT) was serially diluted and captured by Mouse Anti-Rat TIMP‑1 Monoclonal Antibody (Catalog # MAB5802) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Rat TIMP‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF580) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 580-RT) was serially diluted and captured by Mouse Anti-Rat TIMP‑1 Monoclonal Antibody (Catalog # MAB5802) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Rat TIMP‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF580) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />