| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-74520 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-27221 | |||
| BSA Free | NBP2-27220SS | |||
| Azide and BSA Free | NBP2-27221SS | |||
| Azide and BSA Free | NBP2-27221-0.5mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-79836 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-11989-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-24217 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-89462-50ul | |||
![N/A TIM-3 [Biotin]](http://images.novusbio.com/fullsize2/DATA_TIM3_DY2365_ELISA_1818.jpg "N/A TIM-3 [Biotin]")
![Western Blot: TIM-3 Antibody (2E2) [NBP2-45933] - HEK293T cells were transfected with the pCMV6- ENTRY control (Left lane) or pCMV6-ENTRY HAVCR2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti HAVCR2.(1:2000)](http://images.novusbio.com/fullsize/TIM-3-Antibody-2E2-Western-Blot-NBP2-45933-img0009.jpg "Western Blot: TIM-3 Antibody (2E2) [NBP2-45933] - HEK293T cells were transfected with the pCMV6- ENTRY control (Left lane) or pCMV6-ENTRY HAVCR2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti HAVCR2.(1:2000)")
![Immunohistochemistry: TIM-3 Antibody (2E2) [NBP2-45933] - Analysis of Human lymph node tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/TIM-3-Antibody-2E2-Immunohistochemistry-NBP2-45933-img0005.jpg)
![Immunohistochemistry: TIM-3 Antibody (2E2) [NBP2-45933] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120 degrees C for 3min)](http://images.novusbio.com/fullsize/TIM-3-Antibody-2E2-Immunohistochemistry-NBP2-45933-img0001.jpg)
![Immunohistochemistry: TIM-3 Antibody (2E2) [NBP2-45933] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/TIM-3-Antibody-2E2-Immunohistochemistry-NBP2-45933-img0002.jpg)
![Immunohistochemistry: TIM-3 Antibody (2E2) [NBP2-45933] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/TIM-3-Antibody-2E2-Immunohistochemistry-NBP2-45933-img0003.jpg)
![Immunohistochemistry: TIM-3 Antibody (2E2) [NBP2-45933] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/TIM-3-Antibody-2E2-Immunohistochemistry-NBP2-45933-img0004.jpg)
![Immunohistochemistry: TIM-3 Antibody (2E2) [NBP2-45933] - Analysis of Human lymphoma tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)](http://images.novusbio.com/fullsize/TIM-3-Antibody-2E2-Immunohistochemistry-NBP2-45933-img0006.jpg)
![Flow Cytometry: TIM-3 Antibody (F38-2E2) [NBP2-27220] - Cell surface staining of unstimulated (A) and Con A stimulated (B) human PBMCs with purified human TIM-3 antibody (Red) and mouse IgG1 isotype control (Green)and PE-conjugated secondary antibody this antibody was used for this test (cells were not fixed for testing).](http://images.novusbio.com/fullsize/TIM-3-Antibody-F38-2E2-Flow-Cytometry-NBP2-27220-img0002.jpg "Flow Cytometry: TIM-3 Antibody (F38-2E2) [NBP2-27220] - Cell surface staining of unstimulated (A) and Con A stimulated (B) human PBMCs with purified human TIM-3 antibody (Red) and mouse IgG1 isotype control (Green)and PE-conjugated secondary antibody this antibody was used for this test (cells were not fixed for testing).")
![Flow (Cell Surface): TIM-3 Antibody (F38-2E2) [NBP2-27220] - Analysis using the PE conjugate of NBP2-27220. Staining of Con A stimulated human PBMCs with PE-conjugated human TIM-3 antibody (Red) and mouse IgG1 isotype control (Green)was used for this test (cells were not fixed for testing).](http://images.novusbio.com/fullsize/TIM-3-Antibody-F38-2E2-Flow-Cell-Surface-NBP2-27220-img0004.jpg)
![<b>Biological Strategies Validation. </b>Flow (Cell Surface): TIM-3 Antibody (F38-2E2) [NBP2-27220] - hPBMC cells were either stimulated with Con A (panel B) or grown in normal media (panel A). A cell surface stain was performed with TIM-3 (F38-2E2) NBP2-27220 and Mouse IgG1 Kappa NBP2-27287. Cells were incubated with antibody for 20 minutes. A co-stain was also performed with CD4 (RPA-T4) NBP2-27216AF647.](http://images.novusbio.com/fullsize/TIM-3-Antibody-F38-2E2-Flow-Cell-Surface-NBP2-27220-img0005.jpg)
![Immunohistochemistry-Paraffin: TIM-3 Antibody (TIM3/3113) [NBP2-79702] - Formalin-fixed, paraffin-embedded human Tonsil stained with TIM-3 Antibody (TIM3/3113).](http://images.novusbio.com/fullsize/TIM-3-Antibody-TIM3-3113-Immunohistochemistry-Paraffin-NBP2-79702-img0003.jpg "Immunohistochemistry-Paraffin: TIM-3 Antibody (TIM3/3113) [NBP2-79702] - Formalin-fixed, paraffin-embedded human Tonsil stained with TIM-3 Antibody (TIM3/3113).")
![Immunohistochemistry-Paraffin: TIM-3 Antibody (TIM3/3113) [NBP2-79702] - Formalin-fixed, paraffin-embedded human Adrenal stained with TIM-3 Antibody (TIM3/3113).](http://images.novusbio.com/fullsize/TIM-3-Antibody-TIM3-3113-Immunohistochemistry-Paraffin-NBP2-79702-img0001.jpg)
![Immunohistochemistry-Paraffin: TIM-3 Antibody (TIM3/3113) [NBP2-79702] - Formalin-fixed, paraffin-embedded human Tonsil stained with TIM-3 Antibody (TIM3/3113).](http://images.novusbio.com/fullsize/TIM-3-Antibody-TIM3-3113-Immunohistochemistry-Paraffin-NBP2-79702-img0002.jpg)
![Protein Array: TIM-3 Antibody (TIM3/3113) [NBP2-79702] - Analysis of Protein Array containing more than 19,000 full-length human proteins using TIM-3 Antibody (TIM3/3113) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/TIM-3-Antibody-TIM3-3113-Protein-Array-NBP2-79702-img0004.jpg)
![Immunohistochemistry-Paraffin: TIM-3 Antibody (TIM3/3113) [NBP2-79702] - TIM-3 was stained in human FFPE head and neck squamous cell carcinoma tissue. HIER antigen retrieval for 20min at pH 9. AF750 conjugated version of antibody was used (Catalog # NBP2-79836AF750). Image from a verified customer review.](http://images.novusbio.com/fullsize/nbp2-79702_mouse-monoclonal-tim-3-antibody-tim3-3113-17720241044615.jpg)
![Immunocytochemistry/Immunofluorescence: TIM-3 Antibody (2C12) - Chimeric [NBP3-11989] - Immunofluorescence analysis of paraformaldehyde fixed RAW264.7 cells on Shi-fix(TM) coverslips stained with the chimeric rabbit IgG version of 2C12 (NBP3-11989) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-11989, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/TIM-3-Antibody-2C12-Chimeric-Immunocytochemistry-Immunofluorescence-NBP3-11989-img0001.jpg "Immunocytochemistry/Immunofluorescence: TIM-3 Antibody (2C12) - Chimeric [NBP3-11989] - Immunofluorescence analysis of paraformaldehyde fixed RAW264.7 cells on Shi-fix(TM) coverslips stained with the chimeric rabbit IgG version of 2C12 (NBP3-11989) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-11989, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.")
![ELISA: TIM-3 Antibody (2C12) - Chimeric - Azide and BSA Free [NBP3-11989] - Binding curves of NBP3-11989 (blue line) and isotype control (NBP2-52638; red line) to an ELISA plate coated with mouse TIM-3-Fc fusion protein at a concentration of 5 ug/ml. A 3-fold serial dilution from 3000 to 0.0169 ng/ml was performed using NBP3-11989. For signal detection, anti-rabbit IgG1 HRP antibody was used.](http://images.novusbio.com/fullsize/nbp3-11989_rabbit-monoclonal-tim-3-antibody-2c12-chimeric-74202522113036.png)
![Flow Cytometry: TIM-3 Antibody (2C12) - Chimeric - Azide and BSA Free [NBP3-11989] - Paraformaldehyde fixed mouse splenocytes permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (isotype control, black line) or NBP3-11989 (blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/nbp3-11989_rabbit-monoclonal-tim-3-antibody-2c12-chimeric-74202522113017.png)
![Western Blot: TIM-3 Overexpression Lysate (Adult Normal) [NBL1-11452] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for TIM-3.](http://images.novusbio.com/fullsize/TIM-3-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-11452-img0002.jpg "Western Blot: TIM-3 Overexpression Lysate (Adult Normal) [NBL1-11452] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for TIM-3.")