| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-1936SS | |||
| Azide and BSA Free | NBP2-80955 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-17675-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-46712-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide Free | NBP3-11944-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26312-50ul | |||
![Immunohistochemistry: SMN Antibody (2B1) [NB100-1936] - CBs lose their colocalization with SMN nuclear foci in diabetic mice. Normal and pathological distribution of SMN proteins in sensory neurons. SMN proteins were found throughout the cytoplasm and as nuclear foci in control sensory neurons. SMN nuclear foci localized within CBs (coilin) in controls (upper panels, white arrows), whereas in the diabetic nucleus the numerous CBs were present but lost their colocalization with SMN nuclear foci (lower panels, yellow arrows). Arrowheads indicate sensory neurons magnified in the insets. Scale bars: 20 um, 10 um in insets. Image collected and cropped by CiteAb from the following publication (//dmm.biologists.org/lookup/doi/10.1242/dmm.028225), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/SMN-Antibody-2B1-Immunohistochemistry-NB100-1936-img0007.jpg "Immunohistochemistry: SMN Antibody (2B1) [NB100-1936] - CBs lose their colocalization with SMN nuclear foci in diabetic mice. Normal and pathological distribution of SMN proteins in sensory neurons. SMN proteins were found throughout the cytoplasm and as nuclear foci in control sensory neurons. SMN nuclear foci localized within CBs (coilin) in controls (upper panels, white arrows), whereas in the diabetic nucleus the numerous CBs were present but lost their colocalization with SMN nuclear foci (lower panels, yellow arrows). Arrowheads indicate sensory neurons magnified in the insets. Scale bars: 20 um, 10 um in insets. Image collected and cropped by CiteAb from the following publication (//dmm.biologists.org/lookup/doi/10.1242/dmm.028225), licensed under a CC-BY license.")
![Western Blot: SMN Antibody (2B1) [NB100-1936] - Analysis of SMN expression in 1) HeLa, 2) NTera2, 3) HepG2, 4) MCF7, 5) NIH 3T3, 6) PC12, and 7) COS7 whole cell lysates.](http://images.novusbio.com/fullsize/SMN-Antibody-2B1-Western-Blot-NB100-1936-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: SMN Antibody (2B1) [NB100-1936] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.5% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti- NB100-1936 at 2 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/SMN-Antibody-2B1-Immunocytochemistry-Immunofluorescence-NB100-1936-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: SMN Antibody (2B1) [NB100-1936] - scIF of control (f) and etv5b MO (g) cells from pmnx1:eGFP embryos. H2B, GFP and Smn signals are shown in Z-projected confocal sections. GFP+ cells marked by white rectangles are magnified on the right. Scale bars: 10 um for low and 5 um for high magnification. Image collected and cropped by Citeab from the following publication (Transcriptional enhancement of Smn levels in motoneurons is crucial for proper axon morphology in zebrafish. Sci Rep (2016) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/SMN-Antibody-2B1-Immunocytochemistry-Immunofluorescence-NB100-1936-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: SMN Antibody (2B1) [NB100-1936] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with SMN Antibody [2B1] conjugated to Alexa Fluor 488 (NB100-1936AF488) at 5 ug/ml for 1 hour at room temperature. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/SMN-Antibody-2B1-Immunocytochemistry-Immunofluorescence-NB100-1936-img0012.jpg)
![Flow Cytometry: SMN Antibody (2B1) [NB100-1936] - An intracellular stain was performed on Neuro2a cells with SMN Antibody (2B1) NB100-1936 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (35503, Thermo Fisher).](http://images.novusbio.com/fullsize/SMN-Antibody-2B1-Flow-Cytometry-NB100-1936-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: SMN Antibody (2B1) [NB100-1936] - The SMN antibody was tested at a 1:250 dilution in HeLa cells against DyLight 488 (Green). Actin nuclei were counterstained against Phalloidin 568 (Red) and DAPI (Blue), respectively.](http://images.novusbio.com/fullsize/SMN-Antibody-2B1-Immunocytochemistry-Immunofluorescence-NB100-1936-img0004.jpg)
![Immunohistochemistry: SMN Antibody (2B1) [NB100-1936] - Analysis of SMN on mouse brain using NB100-1936.](http://images.novusbio.com/fullsize/SMN-Antibody-2B1-Immunohistochemistry-NB100-1936-img0001.jpg)
![Flow Cytometry: SMN Antibody (2B1) [NB100-1936] - An intracellular stain was performed on Ntera2 cells with SMN Antibody (2B1) NB100-1936 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (35503, Thermo Fisher).](http://images.novusbio.com/fullsize/SMN-Antibody-2B1-Flow-Cytometry-NB100-1936-img0008.jpg)
![Immunocytochemistry/ Immunofluorescence: SMN Antibody (2B1) [NB100-1936] - CWC22 is colocalized with nuclear speckles & upregulated in diabetic DRG sensory neurons. (A) Subcellular distribution of TDP-43 & CWC22 in control DRG sensory neurons. TDP-43 was stained diffusely in the nucleus, excluding SMN foci in sensory neurons. CWC22 consistently colocalized with a marker protein SC35 of nuclear speckles in sensory neurons. No obvious differences in the subcellular localization of CWC22 were identified in diabetic neurons (not shown) compared with controls. Scale bar: 10 μm. (B) qRT-PCR analysis of Cwc22 mRNA expression in diabetic & control mice. Cwc22 expression was upregulated ∼2.5-fold in diabetic DRGs. *P<0.05, unpaired two-tailed Student's t-test. Data represented as mean±s.e.m. See Cheng et al. (2015) for microarray data indicating rises in Cwc22 expression as reported separately. Image collected & cropped by CiteAb from the following publication (//journals.biologists.com/dmm/article/10/3/215/2257/Diabetic-polyneuropathy-sensory-neurons-nuclear), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-1936_mouse-monoclonal-smn-antibody-2b1-3102024168396.jpg)
![Immunocytochemistry/Immunofluorescence: SMN Antibody [NBP3-13414] - SMN antibody detects SMN protein at cytoplasm by immunofluorescent analysis. Sample: Neuro2A cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SMN stained by SMN antibody (NBP3-13414) diluted at 1:500.](http://images.novusbio.com/fullsize/SMN-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-13414-img0002.jpg "Immunocytochemistry/Immunofluorescence: SMN Antibody [NBP3-13414] - SMN antibody detects SMN protein at cytoplasm by immunofluorescent analysis. Sample: Neuro2A cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SMN stained by SMN antibody (NBP3-13414) diluted at 1:500.")
![Western Blot: SMN Antibody [NBP3-13414] - Mouse tissue extract (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with SMN (NBP3-13414) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13414_rabbit-smn-pab-91020242012209.jpg)
![Western Blot: SMN Antibody [NBP3-13414] - Rat tissue extract (50 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with SMN antibody (NBP3-13414) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13414_rabbit-smn-pab-91020242001010.jpg)
![Western Blot: SMN Antibody (9N9Q6) [NBP3-16863] - Western blot analysis of extracts of various cell lines, using SMN antibody (NBP3-16863) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/SMN-Antibody-9N9Q6-Western-Blot-NBP3-16863-img0003.jpg "Western Blot: SMN Antibody (9N9Q6) [NBP3-16863] - Western blot analysis of extracts of various cell lines, using SMN antibody (NBP3-16863) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunohistochemistry-Paraffin: SMN Antibody (9N9Q6) [NBP3-16863] - Immunohistochemistry of paraffin-embedded human lung cancer using SMN Rabbit mAb (NBP3-16863) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/SMN-Antibody-9N9Q6-Immunohistochemistry-Paraffin-NBP3-16863-img0002.jpg)
![Immunohistochemistry-Paraffin: SMN Antibody (9N9Q6) [NBP3-16863] - Immunohistochemistry of paraffin-embedded rat brain using SMN Rabbit mAb (NBP3-16863) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/SMN-Antibody-9N9Q6-Immunohistochemistry-Paraffin-NBP3-16863-img0001.jpg)
![Western Blot: SMN Antibody [NBP3-17675] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10; Lane 2: RT4; Lane 3: U-251 MG; Lane 4: Human Plasma; Lane 5: Liver; Lane 6: Tonsil](http://images.novusbio.com/fullsize/SMN-Antibody-Western-Blot-NBP3-17675-img0003.jpg "Western Blot: SMN Antibody [NBP3-17675] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10; Lane 2: RT4; Lane 3: U-251 MG; Lane 4: Human Plasma; Lane 5: Liver; Lane 6: Tonsil")
![Immunocytochemistry/Immunofluorescence: SMN Antibody [NBP3-17675] - Staining of human cell line HEK 293 shows localization to nuclear bodies & cytosol.](http://images.novusbio.com/fullsize/SMN-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-17675-img0002.jpg)
![Western Blot: SMN Antibody [NBP2-46712] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10, Lane 2: Human cell line RT-4](http://images.novusbio.com/fullsize/SMN-Antibody-Western-Blot-NBP2-46712-img0002.jpg "Western Blot: SMN Antibody [NBP2-46712] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10, Lane 2: Human cell line RT-4")
![Immunohistochemistry-Paraffin: SMN Antibody [NBP2-46712] - Staining of human gallbladder shows nuclear positivity in glandular cells.](http://images.novusbio.com/fullsize/SMN-Antibody-Immunohistochemistry-Paraffin-NBP2-46712-img0003.jpg)
![Western Blot: SMN Antibody [NBP1-32021] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with SMN1 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/SMN-Antibody-Western-Blot-NBP1-32021-img0004.jpg "Western Blot: SMN Antibody [NBP1-32021] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with SMN1 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: SMN Antibody [NBP1-32021] - Analysis of methanol-fixed HeLa, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/SMN-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-32021-img0002.jpg)
![Western Blot: SMN Antibody (4B7) [NBP3-11944] - Western blot of T47D cell lysate showing specific immunolabeling of the ~35 kDa survival motor neuron (SMN) protein.](http://images.novusbio.com/fullsize/SMN-Antibody-4B7-Western-Blot-NBP3-11944-img0001.jpg "Western Blot: SMN Antibody (4B7) [NBP3-11944] - Western blot of T47D cell lysate showing specific immunolabeling of the ~35 kDa survival motor neuron (SMN) protein.")
![Western Blot: SMN Antibody [NBP1-03326] - Detection of Human SMN1 on HeLa whole cell lysate using NBP1-03326. SMN1 was also immunoprecipitated by rabbit anti-SMN1 antibody NBP1-03325.](http://images.novusbio.com/fullsize/SMN-Antibody-Western-Blot-NBP1-03326-img0006.jpg "<b>Independent Antibodies Validation and Biological Strategies Validation.</b>Western Blot: SMN Antibody [NBP1-03326] - Detection of Human SMN1 on HeLa whole cell lysate using NBP1-03326. SMN1 was also immunoprecipitated by rabbit anti-SMN1 antibody NBP1-03325.")
![ELISA: Human, Mouse SMN ELISA Kit (Colorimetric) [NBP2-62175] - A typical standard curve for SMN ELISA kit. These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.](http://images.novusbio.com/fullsize/Human-Mouse-SMN-ELISA-Kit-Colorimetric-ELISA-NBP2-62175-img0002.jpg "ELISA: Human, Mouse SMN ELISA Kit (Colorimetric) [NBP2-62175] - A typical standard curve for SMN ELISA kit. These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.")
![ELISA: Human, Mouse SMN ELISA Kit (Colorimetric) [NBP2-62175] - A parallelism experiment was carried out to determine if the recombinant human SMN standard accurately determines SMN concentrations in biological matrices. To assess parallelism, values for human PBMC lysate and mouse tissue extract was obtained from a s](http://images.novusbio.com/fullsize/Human-Mouse-SMN-ELISA-Kit-Colorimetric-ELISA-NBP2-62175-img0001.jpg)
![Western Blot: SMN Overexpression Lysate (Adult Normal) [NBL1-16243] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for SMN.](http://images.novusbio.com/fullsize/SMN-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-16243-img0002.jpg "Western Blot: SMN Overexpression Lysate (Adult Normal) [NBL1-16243] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for SMN.")
![Western Blot: SMN Overexpression Lysate (Adult Normal) [NBP2-06396] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for SMN.](http://images.novusbio.com/fullsize/SMN-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-06396-img0001.jpg "Western Blot: SMN Overexpression Lysate (Adult Normal) [NBP2-06396] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for SMN.")
![Immunohistochemistry: SMN Antibody (8B10) [NBP3-26312] - Image of SMN Antibody (8B10) diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26312_rabbit-smn-mab-8b10-26220241522418.jpg "Immunohistochemistry: SMN Antibody (8B10) [NBP3-26312] - Image of SMN Antibody (8B10) diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.")