| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-524SS | |||
| Azide and BSA Free | NBP2-80883 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-27328SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-27118SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-48752-25ul | |||
![Western Blot: NOD2 Antibody (2D9) [NB100-524] - HCMV infection induces NOD2 mRNA and protein in HFFs and U373 cells. E. U373 glioma cells were infected with HCMV Towne strain and levels of NOD1, NOD2 and GAPDH mRNAs were measured by qRT-PCR at indicated time points. F. HFFs were infected with HCMV (Towne) at MOI of 1 PFU/cell and levels of NOD2 protein and B-actin were determined 48 and 72 hpi. G. HFFs were infected with HCMV (Towne) strain at MOI of 0.03 or 3 PFU/cell and levels of NOD2 protein and B-actin were determined at 48 hpi. Quantitative data represent mean values (+/-SD) of triplicate determinations from three independent experiments (*p<0.05, **p<0.01, ***p<0.001, one-way ANOVA test). Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0092704.g001) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/NOD2-Antibody-2D9-Western-Blot-NB100-524-img0018.jpg "Western Blot: NOD2 Antibody (2D9) [NB100-524] - HCMV infection induces NOD2 mRNA and protein in HFFs and U373 cells. E. U373 glioma cells were infected with HCMV Towne strain and levels of NOD1, NOD2 and GAPDH mRNAs were measured by qRT-PCR at indicated time points. F. HFFs were infected with HCMV (Towne) at MOI of 1 PFU/cell and levels of NOD2 protein and B-actin were determined 48 and 72 hpi. G. HFFs were infected with HCMV (Towne) strain at MOI of 0.03 or 3 PFU/cell and levels of NOD2 protein and B-actin were determined at 48 hpi. Quantitative data represent mean values (+/-SD) of triplicate determinations from three independent experiments (*p<0.05, **p<0.01, ***p<0.001, one-way ANOVA test). Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0092704.g001) licensed under a CC-BY license.")
![Immunohistochemistry-Frozen: NOD2 Antibody (2D9) [NB100-524] - Overlay of NOD2-DyLight 488 (green) with phase contrast of murine colon. Image from verified customer review.](http://images.novusbio.com/fullsize/NOD2-Antibody-2D9-Immunohistochemistry-Frozen-NB100-524-img0016.jpg)
![Flow (Intracellular): NOD2 Antibody (2D9) [NB100-524] - An intracellular stain was performed on THP-1 cells with NOD2 (2D9) antibody NB100-524AF488 (blue) and a matched isotype control NBP2-27287AF488 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes.](http://images.novusbio.com/fullsize/NOD2-Antibody-2D9-Flow-Intracellular-NB100-524-img0010.jpg)
![Western Blot: NOD2 Antibody (2D9) - BSA Free [NB100-524]](http://images.novusbio.com/fullsize/NOD2-Antibody-2D9-BSA-Free-Western-Blot-NB100-524-img0021.jpg)
![Immunocytochemistry/Immunofluorescence: NOD2 Antibody (2D9) [NB100-524] - NOD2 was detected in human monocytes using NOD2 antibody (2D9) [DyLight 488 (NB100-524G)]- with a concentration of 1:500 in PBS for 2 hours. Image from verified customer review.](http://images.novusbio.com/fullsize/NOD2-Antibody-2D9-Immunocytochemistry-Immunofluorescence-NB100-524-img0020.jpg)
![Immunohistochemistry: NOD2 Antibody (2D9) [NB100-524] - Analysis of PFA fixed mouse sciatic nerve section using anti-NOD2 antibody. Image from verified customer review.](http://images.novusbio.com/fullsize/NOD2-Antibody-2D9-Immunohistochemistry-NB100-524-img0007.jpg)
![Flow Cytometry: NOD2 Antibody (2D9) [NB100-524] - An intracellular stain was performed on THP-1 cells with NOD2 Antibody (2D9) NB100-524 (blue) and a matched mouse IgG1 isotype control (orange) MAB002. Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (35503, Thermo Fisher).](http://images.novusbio.com/fullsize/NOD2-Antibody-2D9-Flow-Cytometry-NB100-524-img0019.jpg)
![Western Blot: NOD2 Antibody (2D9) [NB100-524] - Whole cell protein from THP-1 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2 ug/ml anti-NOD2 in 1% milk, and detected with an anti-mouse HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize/NOD2-Antibody-2D9-Western-Blot-NB100-524-img0017.jpg)
![Flow Cytometry: NOD2 Antibody (2D9) [NB100-524] - An intracellular stain was performed on Jurkat cells with NOD2 (2D9) antibody NB100-524APC (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes.](http://images.novusbio.com/fullsize/NOD2-Antibody-2D9-Flow-Cytometry-NB100-524-img0014.jpg)
![Flow Cytometry: NOD2 Antibody (2D9) [NB100-524] - An intracellular stain was performed on Jurkat cells with NOD2 (2D9) antibody NB100-524PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes.Both antibodies were conjugated to phycoerythrin.](http://images.novusbio.com/fullsize/NOD2-Antibody-2D9-Flow-Cytometry-NB100-524-img0015.jpg)
![Western Blot: NOD2 Antibody (2D9) - BSA Free [NB100-524] - Overexpression of NOD2 restricts HCMV replication & induces antiviral & pro-inflammatory cytokines. B. Cell lysates from 4A used to determine protein expression of HCMV-immediate early (IE1/IE2), early (UL44), & late (pp65) genes. Levels of NOD2 & RIPK2 proteins measured to confirm NOD2 overexpression; beta -actin served as loading control. WB data representative of 3 independent experiments. Asterisks (*) denote endogenous NOD2 & RIPK2 proteins. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0092704), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-524_mouse-monoclonal-nod2-antibody-2d9-31020241535687.jpg)
![Western Blot: NOD2 Antibody [NBP2-27328] - Analysis of NOD2 using this antibody at 1:2000. 30 ug total protein was loaded per lane. Samples from human tumor cell lines: PPC-1 (prostate carcinoma), NCI-H226 and NCI-H332M (lung carcinoma), SF-268 (glioblastoma), UO-31 and A498 (renal/kidney carcinoma), SK-MEL-5 and SK-MEL-28 (melanoma), and MCF-7 (breast carcinoma). Lymphoma is from a cell line generated from a tumor growing in nude mice following injection of primary human lymphoma cells.](http://images.novusbio.com/fullsize/NOD2-Antibody-Western-Blot-NBP2-27328-img0001.jpg "Western Blot: NOD2 Antibody [NBP2-27328] - Analysis of NOD2 using this antibody at 1:2000. 30 ug total protein was loaded per lane. Samples from human tumor cell lines: PPC-1 (prostate carcinoma), NCI-H226 and NCI-H332M (lung carcinoma), SF-268 (glioblastoma), UO-31 and A498 (renal/kidney carcinoma), SK-MEL-5 and SK-MEL-28 (melanoma), and MCF-7 (breast carcinoma). Lymphoma is from a cell line generated from a tumor growing in nude mice following injection of primary human lymphoma cells.")
![Immunohistochemistry-Paraffin: NOD2 Antibody [NBP2-27328] - Formalin-fixed, paraffin-embedded normal human colon stained for NOD2 expression using this antibody at 1:2000. A, tissue core from a colon tissue microarray. A1, high magnification of A. Hematoxylin-eosin counterstain.](http://images.novusbio.com/fullsize/NOD2-Antibody-Immunohistochemistry-Paraffin-NBP2-27328-img0002.jpg)
![Immunohistochemistry-Paraffin: NOD2 Antibody [NBP2-48752] - Analysis in human skin and kidney tissues. Corresponding NOD2 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/NOD2-Antibody-Immunohistochemistry-Paraffin-NBP2-48752-img0013.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: NOD2 Antibody [NBP2-48752] - Analysis in human skin and kidney tissues. Corresponding NOD2 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: NOD2 Antibody [NBP2-48752] - Staining of human skin shows moderate to strong cytoplasmic positivity in keratinocytes.](http://images.novusbio.com/fullsize/NOD2-Antibody-Immunohistochemistry-Paraffin-NBP2-48752-img0009.jpg)
![Immunohistochemistry-Paraffin: NOD2 Antibody [NBP2-48752] - Staining of human duodenum shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/NOD2-Antibody-Immunohistochemistry-Paraffin-NBP2-48752-img0010.jpg)
![Immunohistochemistry-Paraffin: NOD2 Antibody [NBP2-48752] - Staining of human rectum shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/NOD2-Antibody-Immunohistochemistry-Paraffin-NBP2-48752-img0011.jpg)
![Immunohistochemistry-Paraffin: NOD2 Antibody [NBP2-48752] - Staining of human kidney shows no positivity in cells in tubules as expected.](http://images.novusbio.com/fullsize/NOD2-Antibody-Immunohistochemistry-Paraffin-NBP2-48752-img0012.jpg)
![Immunohistochemistry-Paraffin: NOD2 Antibody (hNOD2.017) [NBP3-11219] - NOD2 on human jejunum. Staining of paraffin tissue section with NBP3-11219 with a dilution of 100x.](http://images.novusbio.com/fullsize/NOD2-Antibody-hNOD2-017-Immunohistochemistry-Paraffin-NBP3-11219-img0002.jpg "Immunohistochemistry-Paraffin: NOD2 Antibody (hNOD2.017) [NBP3-11219] - NOD2 on human jejunum. Staining of paraffin tissue section with NBP3-11219 with a dilution of 100x.")
![Immunohistochemistry-Paraffin: NOD2 Antibody (hNOD2.017) [NBP3-11219] - NOD2 on human jejunum. Staining of paraffin tissue section with NBP3-11219 with a dilution of 100x.](http://images.novusbio.com/fullsize/NOD2-Antibody-hNOD2-017-Immunohistochemistry-Paraffin-NBP3-11219-img0001.jpg)
![Western Blot: NOD2 Overexpression Lysate (Adult Normal) [NBP2-06456] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for NOD2.](http://images.novusbio.com/fullsize/NOD2-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-06456-img0001.jpg "Western Blot: NOD2 Overexpression Lysate (Adult Normal) [NBP2-06456] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for NOD2.")