CX3CL1/Fractalkine: Novus Biologicals

AF365

CX3CL1/Fractalkine Antibody [Unconjugated]

(2 Reviews)

100 μg

AF365

365-FR) chemoattracts the BaF3 mouse pro-B cell line transfected with mouse CX3CR1 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CX3CL1/Fractalkine (100 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human CX3CL1/Fractalkine Chemokine Domain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF365). The ND₅₀ is typically 0.75-4.5 µg/mL." class="big_lightbox">

Recombinant Human CX3CL1/Fractalkine (Catalog # <a class=

365-FR) chemoattracts the BaF3 mouse pro-B cell line transfected with mouse CX3CR1 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CX3CL1/Fractalkine (100 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human CX3CL1/Fractalkine Chemokine Domain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF365). The ND₅₀ is typically 0.75-4.5 µg/mL." alt="Recombinant Human CX3CL1/Fractalkine (Catalog # 365-FR) chemoattracts the BaF3 mouse pro-B cell line transfected with mouse CX3CR1 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CX3CL1/Fractalkine (100 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human CX3CL1/Fractalkine Chemokine Domain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF365). The ND₅₀ is typically 0.75-4.5 µg/mL." class="big_thumb" />

CX3CR1 is required for keratinocytes differentiation. (A) Expression of CX3CR1 in parental PA-1 cell line&that stably transfected with either CX3CR1 or scrambled shRNA constructs was examined with WB. Actin served as a loading control. CX3CR1 expression levels quantified using digital densitometry&normalized to the levels of actin expression. (B) Expression of cytokeratin 14 in PA-1 cells stably transfected with either CX3CR1 or scrambled shRNA constructs treated with recombinant BMP-4 or vehicle, as indicated, was examined with immunocytochemistry. CK14 in PA-1 was probed with anti-CK14&Alexa555-conjugated anti-rabbit antibodies,&DNA was stained with 4’,6-Diamidino-2-Phenylindole, Dihydrochloride (DAPI); CK14 – red, DNA – blue. Images taken using 5× magnification on the objective. The histogram demonstrates the average intensity of CK14 signal across the field as determined by the Zeiss AxioVision software. Data is an average of three independent experiments. *p < 0.05. (C) Expression of cytokeratins 14&18 in PA-1 cells stably transfected with either CX3CR1 or scrambled shRNA constructs treated with recombinant BMP-4 or vehicle, as indicated, was examined with WB. beta -Tubulin served as a loading control. The histogram shows CK14&CK18 expression levels. Expression of CK18 in CX3CR1shRNA-transfected PA-1 cells was arbitrarily set as 1&expression of both CK14&CK18 in other conditions was calculated accordingly. The data represent a typical WB image,&quantitative analysis was performed using three independently performed experiments. *p < 0.05. (D) Expression of CX3CL1 in SKOV-3&PA-1 cell lines was examined with WB. Actin served as a loading control. SKOV-3 cell line was used as a positive control. Image collected&cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/23958497), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF365U-100UG
Alexa Fluor 405	AF365V-100UG
Alexa Fluor 488	AF365G-100UG
Alexa Fluor 532	AF365X-100UG
Alexa Fluor 594	AF365T-100UG
Alexa Fluor 647	AF365R-100UG
Alexa Fluor 700	AF365N-100UG
Alexa Fluor 750	AF365S-100UG
Alexa Fluor Plus 405	AF365AFP405-100UG
Alexa Fluor Plus 488	AF365AFP488-100UG
Alexa Fluor Plus 555	AF365AFP555-100UG
Alexa Fluor Plus 594	AF365AFP594-100UG
Alexa Fluor Plus 647	AF365AFP647-100UG
Alexa Fluor Plus 680	AF365AFP680-100UG
Allophycocyanin	AF365A
Biotin	BAF365
CoraFluor 1	AF365CL1
DyLight 350	AF365D
DyLight 405	AF365E
DyLight 488	AF365K
DyLight 550	AF365L
DyLight 594	AF365M
DyLight 650	AF365W
DyLight 680	AF365Y
DyLight 755	AF365Z
FITC	AF365F
HRP	AF365H
Janelia Fluor 525	AF365JF525
Janelia Fluor 549	AF365I
Janelia Fluor 646	AF365J
Janelia Fluor 669	AF365JF669
PE	AF365P
PerCP	AF365C
mFluor Violet 450 SE	AF365MFV450
mFluor Violet 500 SE	AF365MFV500
mFluor Violet 610 SE	AF365MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF365-SP

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AF537

CX3CL1/Fractalkine Antibody [Unconjugated]

(22 Publications)

100 μg

AF537

536-FR) chemoattracts the BaF3 mouse pro-B cell line transfected with human CX3CR1 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Rat CX3CL1/Fractalkine (40 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Rat CX3CL1/Fractalkine Chemokine Domain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF537). The ND₅₀ is typically 0.3-1.2 µg/mL." class="big_lightbox">

Recombinant Rat CX3CL1/Fractalkine (Catalog # <a class=

536-FR) chemoattracts the BaF3 mouse pro-B cell line transfected with human CX3CR1 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Rat CX3CL1/Fractalkine (40 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Rat CX3CL1/Fractalkine Chemokine Domain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF537). The ND₅₀ is typically 0.3-1.2 µg/mL." alt="Recombinant Rat CX3CL1/Fractalkine (Catalog # 536-FR) chemoattracts the BaF3 mouse pro-B cell line transfected with human CX3CR1 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Rat CX3CL1/Fractalkine (40 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Rat CX3CL1/Fractalkine Chemokine Domain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF537). The ND₅₀ is typically 0.3-1.2 µg/mL." class="big_thumb" />

CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_lightbox">

CX3CL1/Fractalkine was detected in perfusion fixed frozen sections of mouse brain (cingulate cortex) using 1.7 µg/mL Goat Anti-Rat CX3CL1/Fractalkine Chemokine Domain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF537) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # <a class=

CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_thumb" />

Upregulation of the expression of CX3CL1 and CX3CR1 in the hippocampus of 2VO and miR-195 loss-of-function rats. a, b Expression of CX3CL1 (red) and CX3CR1 (green) in the hippocampus of sham and 2VO rats was shown by immunofluorescence staining. The scale bar is 500 um. c Expression of CX3CL1 and CX3CR1 in the hippocampus of sham and 2VO rats detected by western blot technique. Bars represent the mean ± SD, n = 6, *P < 0.05 vs sham group, Student’s t test. d Lenti-pre-AMO-miR-195 upregulated the expression of CX3CL1 in rat hippocampus. Mean ± SD, n = 6, *P < 0.05 vs sham; #P < 0.05 vs lenti-pre-AMO-miR-195. Data were analyzed using one-way ANOVA followed by Tukey test. e Lenti-pre-AMO-miR-195 upregulated the expression of CX3CR1 in the rat hippocampus. Mean ± SD, n = 6, *P < 0.05 vs sham; #P < 0.05 vs lenti-pre-AMO-miR-195. Data were analyzed using one-way ANOVA followed by Tukey test Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/32819407), licensed under a CC-BY license. Not internally tested by R&D Systems.

536-FR) was serially diluted and captured by Goat Anti-Rat CX3CL1/Fractalkine Chemokine Domain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF537) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Rat CX3CL1/Fractalkine Chemokine Domain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF537) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox">

536-FR) was serially diluted and captured by Goat Anti-Rat CX3CL1/Fractalkine Chemokine Domain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF537) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Rat CX3CL1/Fractalkine Chemokine Domain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF537) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

536-FR) was serially diluted and captured by Goat Anti-Rat CX3CL1/Fractalkine Chemokine Domain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF537) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Rat CX3CL1/Fractalkine Chemokine Domain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF537) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox">

536-FR) was serially diluted and captured by Goat Anti-Rat CX3CL1/Fractalkine Chemokine Domain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF537) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Rat CX3CL1/Fractalkine Chemokine Domain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF537) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

Reactivity:

Rt

Species Glossary

Reactivity: Rt

Applications:

, , ,

Applications: , , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF537U-100UG
Alexa Fluor 405	AF537V-100UG
Alexa Fluor 488	AF537G-100UG
Alexa Fluor 532	AF537X-100UG
Alexa Fluor 594	AF537T-100UG
Alexa Fluor 647	AF537R-100UG
Alexa Fluor 700	AF537N-100UG
Alexa Fluor 750	AF537S-100UG
Alexa Fluor Plus 405	AF537AFP405-100UG
Alexa Fluor Plus 488	AF537AFP488-100UG
Alexa Fluor Plus 555	AF537AFP555-100UG
Alexa Fluor Plus 594	AF537AFP594-100UG
Alexa Fluor Plus 647	AF537AFP647-100UG
Alexa Fluor Plus 680	AF537AFP680-100UG
Allophycocyanin	AF537A
Biotin	BAF537
CoraFluor 1	AF537CL1
DyLight 350	AF537D
DyLight 405	AF537E
DyLight 488	AF537K
DyLight 550	AF537L
DyLight 594	AF537M
DyLight 650	AF537W
DyLight 680	AF537Y
DyLight 755	AF537Z
FITC	AF537F
HRP	AF537H
Janelia Fluor 525	AF537JF525
Janelia Fluor 549	AF537I
Janelia Fluor 646	AF537J
Janelia Fluor 669	AF537JF669
PE	AF537P
PerCP	AF537C
mFluor Violet 450 SE	AF537MFV450
mFluor Violet 500 SE	AF537MFV500
mFluor Violet 610 SE	AF537MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF537-SP

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DY365

Human CX3CL1/Fractalkine DuoSet ELISA, 15 Plate

(29 Publications)

1 Kit (for 15 Plates)

DY365

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

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DCX310

Human CX3CL1/Fractalkine Quantikine ELISA Kit

(26 Publications)

1 Kit

DCX310

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

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AF472

CX3CL1/Fractalkine Antibody [Unconjugated]

(18 Publications)

100 μg

AF472

571-MF) chemoattracts the BaF3 mouse pro-B cell line transfected with human CX3CR1 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CX3CL1/Fractalkine (30 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse CX3CL1/Fractalkine Chemokine Domain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF472). The ND₅₀ is typically 0.3-1.5 µg/mL." class="big_lightbox">

Recombinant Mouse CX3CL1/Fractalkine (Catalog # <a class=

571-MF) chemoattracts the BaF3 mouse pro-B cell line transfected with human CX3CR1 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CX3CL1/Fractalkine (30 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse CX3CL1/Fractalkine Chemokine Domain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF472). The ND₅₀ is typically 0.3-1.5 µg/mL." alt="Recombinant Mouse CX3CL1/Fractalkine (Catalog # 571-MF) chemoattracts the BaF3 mouse pro-B cell line transfected with human CX3CR1 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CX3CL1/Fractalkine (30 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse CX3CL1/Fractalkine Chemokine Domain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF472). The ND₅₀ is typically 0.3-1.5 µg/mL." class="big_thumb" />

<b>Biological Strategies Validation. </b>Cardiomyocyte-specific ADAM10/CX3CL1 signaling regulates neutrophil migration.a Immunofluorescence images and b quantification of CX3CL1, cardiac Troponin T (cTnT, cardiomyocytes, CM) and CD31 (endothelial cells, EC) stained heart tissue sections of sham-operated (Sham) and LAD-ligated (MI) mice 14 days after surgery. Nuclei are stained with DAPI. Representative images are shown. Scale bar, 20 µm. n = 4 per group, mean ± SEM, ns not significant, two-tailed Mann–Whitney test with Dunn’s posttest. c Co-immunoprecipitation of endogenous ADAM10 (A10) and CX3CL1 from whole-cell lysates of HL-1 cells. Representative western blots are shown (n = 4). IgG, immunoglobulin G as a control. WCL, whole-cell lysate. d Western blot analysis of CX3CL1 and e quantification of CX3CL1 expression (n = 6 per group, mean ± SEM, ns not significant, **P < 0.01, ***P < 0.001, one-way ANOVA with Tukey’s posttest) as well as f ectodomain shedding (CX3CL1 levels in supernatant) in normoxic as well as GI254023X (A10i) and DMSO treated hypoxic (1% O2) mouse cardiomyocytes (HL-1) (n = 3 per group, mean ± SEM, ns not significant, **P < 0.01, Kruskal–Wallis test with Dunn’s posttest, exact P-values are provided in the Source Data file). g Treatment scheme. HL-1 cells were cultured for 3 h under normoxic or hypoxic conditions in parallel to A10i or DMSO treatment in combination with unspecific control siRNA (siC) or CX3CL1 depletion (siCX3CL1). Supernatants were used in the lower chamber and 3 × 105 freshly isolated mouse bone marrow derived neutrophils or macrophages were seeded in the upper chamber of transwell plates (8 µm pore size) to determine migration capacity. The scheme was created using Servier Medical Art (available online: //smart.servier.com). h Western blot analysis and i quantification of CX3CL1 expression in HL-1 cells treated for 48 h with unspecific control siRNA (siC) or CX3CL1-specific siRNA (siCX3#1 and siCX3#2). Representative western blots (n = 3 per group, mean ± SEM, *

$Cardiomyocyte-specific ADAM10 KO improves post-MI cardiac function.a–c Echocardiographic assessment of fractional area shortening, ejection fraction and left ventricular end-systolic interior diameter in 8-week-old ADAM10fl/fl (WT) and ( alpha MHC-Cre) ADAM10 KO mice under a basal conditions (WT, n = 7, KO, n = 8, mean ± SEM, ns not significant) as well as b 3 days (WT, n = 8, KO, n = 9, mean ± SEM, ns not significant, *P < 0.05, two-tailed t test) and c 14 days after myocardial infarction (WT, n = 6, KO, n = 9, mean ± SEM, ns not significant, *P < 0.05, two-tailed t test, exact P-values are provided in the Source Data file). d Representative end-systolic B-mode and M-mode echocardiograms of ADAM10 WT and ADAM10 KO mice 14 days after myocardial infarction. e Western blot analysis and f quantification of ADAM10, CX3CL1 and IL-1 beta expression in heart tissue lysates of ADAM10 WT and ADAM10 KO mice 3 days after myocardial infarction (n = 6, mean ± SEM, ns not significant, *P < 0.05, **P < 0.01, two-tailed t test). Exact P-values: ADAM10, 0.0159. CX3CL1, 0.0084. IL-1 beta , 0.0317. g Analysis of leukocyte counts in blood samples of ADAM10 WT (n = 8) and ADAM10 KO (n = 7) mice (mean ± SEM, ns not significant, *P < 0.05, two-tailed t test). Exact P-values: Neutrophils, 0.0404. Eosinophils, 0.7886. Monocytes, 0.3373. Lymphocytes, 0.751. h Analysis of leukocyte counts in the infarcted area/ infarct border zone of ADAM10 WT (n = 8) and ADAM10 KO (n = 7) mice (mean ± SEM, ns not significant, *P < 0.05, two-tailed two-tailed t test). Exact P-values: Neutrophils, 0.0214. Eosinophils, 0.176. Monocytes, 0.639. Macrophages, 0.6064. Source data are provided as a Source Data file. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36496449), licensed under a CC-BY license. Not internally tested by R&D Systems.$

472-FF) was serially diluted and captured by Rat Anti-Mouse CX3CL1/Fractalkine Chemokine Domain Monoclonal Antibody (Catalog # MAB571) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse CX3CL1/Fractalkine Chemokine Domain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF472) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox">

Recombinant Mouse CX3CL1/Fractalkine Full Length (Catalog # <a class=

472-FF) was serially diluted and captured by Rat Anti-Mouse CX3CL1/Fractalkine Chemokine Domain Monoclonal Antibody (Catalog # MAB571) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse CX3CL1/Fractalkine Chemokine Domain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF472) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

,

Applications: ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF472U-100UG
Alexa Fluor 405	AF472V-100UG
Alexa Fluor 488	AF472G-100UG
Alexa Fluor 532	AF472X-100UG
Alexa Fluor 594	AF472T-100UG
Alexa Fluor 647	AF472R-100UG
Alexa Fluor 700	AF472N-100UG
Alexa Fluor 750	AF472S-100UG
Alexa Fluor Plus 405	AF472AFP405-100UG
Alexa Fluor Plus 488	AF472AFP488-100UG
Alexa Fluor Plus 555	AF472AFP555-100UG
Alexa Fluor Plus 594	AF472AFP594-100UG
Alexa Fluor Plus 647	AF472AFP647-100UG
Alexa Fluor Plus 680	AF472AFP680-100UG
Allophycocyanin	AF472A
Biotin	BAF472
CoraFluor 1	AF472CL1
DyLight 350	AF472D
DyLight 405	AF472E
DyLight 488	AF472K
DyLight 550	AF472L
DyLight 594	AF472M
DyLight 650	AF472W
DyLight 680	AF472Y
DyLight 755	AF472Z
FITC	AF472F
HRP	AF472H
Janelia Fluor 525	AF472JF525
Janelia Fluor 549	AF472I
Janelia Fluor 646	AF472J
Janelia Fluor 669	AF472JF669
PE	AF472P
PerCP	AF472C
mFluor Violet 450 SE	AF472MFV450
mFluor Violet 500 SE	AF472MFV500
mFluor Violet 610 SE	AF472MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF472-SP

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365-FR

Recombinant Human CX3CL1/Fractalkine (Full Length) Protein

(22 Publications)

25 μg

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	365-FR-025/CF

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MAB571

CX3CL1/Fractalkine Antibody (126315) [Unconjugated]

(13 Publications)

100 μg

MAB571

571-MF) chemoattracts the BaF3 mouse pro-B cell line transfected with human CX3CR1 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CX3CL1/Fractalkine (30 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse CX3CL1/Fractalkine Chemokine Domain Monoclonal Antibody (Catalog # MAB571). The ND₅₀ is typically 0.75-3.5 µg/mL." class="big_lightbox">

571-MF) chemoattracts the BaF3 mouse pro-B cell line transfected with human CX3CR1 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CX3CL1/Fractalkine (30 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse CX3CL1/Fractalkine Chemokine Domain Monoclonal Antibody (Catalog # MAB571). The ND₅₀ is typically 0.75-3.5 µg/mL." alt="Recombinant Mouse CX3CL1/Fractalkine (Catalog # 571-MF) chemoattracts the BaF3 mouse pro-B cell line transfected with human CX3CR1 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CX3CL1/Fractalkine (30 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse CX3CL1/Fractalkine Chemokine Domain Monoclonal Antibody (Catalog # MAB571). The ND₅₀ is typically 0.75-3.5 µg/mL." class="big_thumb" />

<b>Biological Strategies Validation. </b>CX3CL1 expression in the brain during EAE progression. (A) Brain CX3CL1 expression of wild type mice with EAE as determined by quantitative real-time PCR. Gene levels were normalized to GAPDH levels and are displayed as levels relative to naïve mice. Error bars represent the s.e.m. (n = 5 mice per group). (B-M) Brains from naïve wild type mice were harvested and frozen for immunostaining. CX3CL1 expression (red) and DAPI nuclei staining (blue) in (B, F, J) naïve and (C, G, K) Day 10, (D, H, L) Day 14, and (E, I, M) Day 21 post-EAE induced wild type mice. CX3CL1 expression is displayed at the (B-E) choroid plexus, (F-I) in and near the hippocampus, and (J-M) cerebellum. White scale bars represent 50 μm. (N) CX3CL1 expression (relative to non-treated cells) in the CPLacZ-2 mouse choroid plexus cell line after 2 hour treatment with varying concentrations of the A2A adenosine receptor specific adenosine receptor agonist CGS21680. Error bars represent the s.e.m. (O) Lymphocyte migration across a transwell choroid plexus barrier following pretreatment with vehicle treatment alone, CGS21680, or CGS21680 and anti-CX3CL1. Total migration was normalized to the vehicle control (set to 100%). Error bars represent the s.e.m. These results are representative of two separate experiments (n ≤ 3). Image collected and cropped by CiteAb from the following publication (//jneuroinflammation.biomedcentral.com/articles/10.1186/1742-2094-9-193), licensed under a CC-BY license. Not internally tested by R&D Systems.

472-FF) was serially diluted and captured by Rat Anti-Mouse CX3CL1/Fractalkine Chemokine Domain Monoclonal Antibody (Catalog # MAB571) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse CX3CL1/Fractalkine Chemokine Domain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF472) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox">

472-FF) was serially diluted and captured by Rat Anti-Mouse CX3CL1/Fractalkine Chemokine Domain Monoclonal Antibody (Catalog # MAB571) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse CX3CL1/Fractalkine Chemokine Domain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF472) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

, ,

Applications: , ,

Host:

Rat Monoclonal

Host: Rat Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB571U
Alexa Fluor 405	FAB571V
Alexa Fluor 532	FAB571X
Alexa Fluor 594	FAB571T
Alexa Fluor 647	FAB571R
Alexa Fluor 700	FAB571N
Alexa Fluor 750	FAB571S
Allophycocyanin	FAB571A-025
Biotin	FAB571B
CoraFluor 1	FAB571CL1
DyLight 350	FAB571D
DyLight 405	FAB571E
DyLight 488	FAB571K
DyLight 550	FAB571L
DyLight 594	FAB571M
DyLight 650	FAB571W
DyLight 680	FAB571Y
DyLight 755	FAB571Z
FITC	FAB571F
HRP	FAB571H
Janelia Fluor 525	FAB571JF525
Janelia Fluor 549	FAB571I
Janelia Fluor 646	FAB571J
Janelia Fluor 669	FAB571JF669
PerCP	FAB571C
Phycoerythrin	FAB571P-025
mFluor Violet 450 SE	FAB571MFV450
mFluor Violet 500 SE	FAB571MFV500
mFluor Violet 610 SE	FAB571MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB571-SP
Carrier Free	FAB571P-025
Carrier Free	FAB571A-025
Carrier Free	MAB571-500

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MAB3651

CX3CL1/Fractalkine Antibody (81513) [Unconjugated]

(1 Review)

(14 Publications)

100 μg

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB3651U-100UG
Alexa Fluor 405	FAB3651V-100UG
Alexa Fluor 488	FAB3651G-100UG
Alexa Fluor 532	FAB3651X-100UG
Alexa Fluor 594	FAB3651T-100UG
Alexa Fluor 647	FAB3651R-100UG
Alexa Fluor 700	FAB3651N-100UG
Alexa Fluor 750	FAB3651S-100UG
Alexa Fluor Plus 405	FAB3651AFP405-100UG
Alexa Fluor Plus 488	FAB3651AFP488-100UG
Alexa Fluor Plus 555	FAB3651AFP555-100UG
Alexa Fluor Plus 594	FAB3651AFP594-100UG
Alexa Fluor Plus 647	FAB3651AFP647-100UG
Alexa Fluor Plus 680	FAB3651AFP680-100UG
Allophycocyanin	FAB3651A
Biotin	FAB3651B
CoraFluor 1	FAB3651CL1
DyLight 350	FAB3651D
DyLight 405	FAB3651E
DyLight 488	FAB3651K
DyLight 550	FAB3651L
DyLight 594	FAB3651M
DyLight 650	FAB3651W
DyLight 680	FAB3651Y
DyLight 755	FAB3651Z
FITC	FAB3651F
HRP	FAB3651H
Janelia Fluor 525	FAB3651JF525
Janelia Fluor 549	FAB3651I
Janelia Fluor 646	FAB3651J
Janelia Fluor 669	FAB3651JF669
PE	FAB3651P
PerCP	FAB3651C
mFluor Violet 450 SE	FAB3651MFV450
mFluor Violet 500 SE	FAB3651MFV500
mFluor Violet 610 SE	FAB3651MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB3651-SP
Carrier Free	MAB3651-500

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MAB3652

CX3CL1/Fractalkine Antibody (81506) [Unconjugated]

(11 Publications)

100 μg

MAB3652

F0101B). View our protocol for Staining Membrane-associated Proteins. " class="big_lightbox">

HEK293 cells transfected with hCX3CL1 (A) vs Irrelevant HEK293 transfectant cells (B) were stained with Mouse Anti-Human CX3CL1/Fractalkine Chemokine Domain Monoclonal Antibody (Catalog # MAB3652) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # <a class=NoLineLink href=

F0101B). View our protocol for Staining Membrane-associated Proteins. " alt="HEK293 cells transfected with hCX3CL1 (A) vs Irrelevant HEK293 transfectant cells (B) were stained with Mouse Anti-Human CX3CL1/Fractalkine Chemokine Domain Monoclonal Antibody (Catalog # MAB3652) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins. " class="big_thumb" />

365-FR) chemoattracts the BaF3 mouse pro-B cell line transfected with mouse CX3CR1 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CX3CL1/Fractalkine (100 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CX3CL1/Fractalkine Chemokine Domain Monoclonal Antibody (Catalog # MAB3652). The ND₅₀ is typically 1-5 µg/mL." class="big_lightbox">

365-FR) chemoattracts the BaF3 mouse pro-B cell line transfected with mouse CX3CR1 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CX3CL1/Fractalkine (100 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CX3CL1/Fractalkine Chemokine Domain Monoclonal Antibody (Catalog # MAB3652). The ND₅₀ is typically 1-5 µg/mL." class="big_thumb" />

365-FR) was serially diluted and captured by Mouse Anti-Human CX3CL1/Fractalkine Chemokine Domain Monoclonal Antibody (Catalog # MAB3652) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human CX3CL1/Fractalkine Chemokine Domain Monoclonal Antibody (Catalog # MAB365) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox">

Recombinant Human CX3CL1/Fractalkine Full Length (Catalog # <a class=

365-FR) was serially diluted and captured by Mouse Anti-Human CX3CL1/Fractalkine Chemokine Domain Monoclonal Antibody (Catalog # MAB3652) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human CX3CL1/Fractalkine Chemokine Domain Monoclonal Antibody (Catalog # MAB365) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	IC3652U-100UG
Alexa Fluor 405	IC3652V-100UG
Alexa Fluor 488	IC3652G-100UG
Alexa Fluor 532	FAB3652X
Alexa Fluor 594	IC3652T-100UG
Alexa Fluor 647	IC3652R-100UG
Alexa Fluor 700	IC3652N-100UG
Alexa Fluor 750	IC3652S-100UG
Alexa Fluor Plus 405	FAB3652AFP405-100UG
Alexa Fluor Plus 488	FAB3652AFP488-100UG
Alexa Fluor Plus 555	FAB3652AFP555-100UG
Alexa Fluor Plus 594	FAB3652AFP594-100UG
Alexa Fluor Plus 647	FAB3652AFP647-100UG
Alexa Fluor Plus 680	FAB3652AFP680-100UG
Allophycocyanin	FAB3652A
Biotin	FAB3652B
CoraFluor 1	FAB3652CL1
DyLight 350	FAB3652D
DyLight 405	FAB3652E
DyLight 488	FAB3652K
DyLight 550	FAB3652L
DyLight 594	FAB3652M
DyLight 650	FAB3652W
DyLight 680	FAB3652Y
DyLight 755	FAB3652Z
FITC	FAB3652F
HRP	FAB3652H
Janelia Fluor 525	FAB3652JF525
Janelia Fluor 549	FAB3652I
Janelia Fluor 646	FAB3652J
Janelia Fluor 669	FAB3652JF669
PE	FAB3652P
PerCP	FAB3652C
mFluor Violet 450 SE	FAB3652MFV450
mFluor Violet 500 SE	FAB3652MFV500
mFluor Violet 610 SE	FAB3652MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB3652-SP
Carrier Free	MAB3652-500
Carrier Free	IC3652V-100UG
Carrier Free	IC3652U-100UG
Carrier Free	IC3652T-100UG
Carrier Free	IC3652S-100UG
Carrier Free	IC3652R-100UG
Carrier Free	IC3652N-100UG
Carrier Free	IC3652G-100UG

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472-FF

Recombinant Mouse CX3CL1/Fractalkine (Full Length) Protein

(14 Publications)

25 μg

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

Applications:

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	472-FF-025/CF

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MCX310

Mouse CX3CL1/Fractalkine Quantikine ELISA Kit

(13 Publications)

1 Kit

MCX310

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

Applications:

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MAB365

CX3CL1/Fractalkine Antibody (51637) [Unconjugated]

(12 Publications)

100 μg

MAB365

F0101B). View our protocol for Staining Membrane-associated Proteins. " class="big_lightbox">

F0101B). View our protocol for Staining Membrane-associated Proteins. " alt="HEK293 cells transfected with hCX3CL1 (A) vs Irrelevant HEK293 transfectant cells (B) were stained with Mouse Anti-Human CX3CL1/Fractalkine Chemokine Domain Monoclonal Antibody (Catalog # MAB365) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins. " class="big_thumb" />

365-FR) was serially diluted and captured by Mouse Anti-Human CX3CL1/Fractalkine Chemokine Domain Monoclonal Antibody (Catalog # MAB3652) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human CX3CL1/Fractalkine Chemokine Domain Monoclonal Antibody (Catalog # MAB365) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox">

365-FR) was serially diluted and captured by Mouse Anti-Human CX3CL1/Fractalkine Chemokine Domain Monoclonal Antibody (Catalog # MAB3652) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human CX3CL1/Fractalkine Chemokine Domain Monoclonal Antibody (Catalog # MAB365) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	IC365U-100UG
Alexa Fluor 405	IC365V-100UG
Alexa Fluor 488	IC365G-100UG
Alexa Fluor 532	FAB365X
Alexa Fluor 594	IC365T-100UG
Alexa Fluor 647	IC365R-100UG
Alexa Fluor 700	IC365N-100UG
Alexa Fluor 750	IC365S-100UG
Alexa Fluor Plus 405	FAB365AFP405-100UG
Alexa Fluor Plus 488	FAB365AFP488-100UG
Alexa Fluor Plus 555	FAB365AFP555-100UG
Alexa Fluor Plus 594	FAB365AFP594-100UG
Alexa Fluor Plus 647	FAB365AFP647-100UG
Alexa Fluor Plus 680	FAB365AFP680-100UG
Allophycocyanin	IC365A
Biotin	BAM365
CoraFluor 1	FAB365CL1
DyLight 350	FAB365D
DyLight 405	FAB365E
DyLight 488	FAB365K
DyLight 550	FAB365L
DyLight 594	FAB365M
DyLight 650	FAB365W
DyLight 680	FAB365Y
DyLight 755	FAB365Z
FITC	FAB365F
HRP	FAB365H
Janelia Fluor 525	FAB365JF525
Janelia Fluor 549	FAB365I
Janelia Fluor 646	FAB365J
Janelia Fluor 669	FAB365JF669
PerCP	FAB365C
Phycoerythrin	IC365P
mFluor Violet 450 SE	FAB365MFV450
mFluor Violet 500 SE	FAB365MFV500
mFluor Violet 610 SE	FAB365MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	IC365P
Carrier Free	IC365A
Carrier Free	MAB365-SP
Carrier Free	MAB365-500
Carrier Free	IC365V-100UG
Carrier Free	IC365U-100UG
Carrier Free	IC365T-100UG
Carrier Free	IC365S-100UG
Carrier Free	IC365R-100UG
Carrier Free	IC365N-100UG
Carrier Free	IC365G-100UG

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Reviews

Host

Available Conjugates

Format

Modification

Clonality

Clone

Gene Symbol

Images Type

Isotype

Preservative

Protein Or Peptide

Research Areas

Has Sample Size

Promotional Item

Species Glossary

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