| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-54153SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-44677 | |||
| Azide and BSA Free | NBP2-34772-0.1mg | |||
NL007) and counterstained with DAPI (blue). Specific staining was localized to the nucleus. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." alt="MyoD was detected in fixed C2C12 mouse myoblast cell line (Positive) and absent in RD cells (Negative) using Mouse Anti-Human MyoD Monoclonal Antibody (Catalog # MAB5966) at 25 µg/ml for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to the nucleus. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB5966-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-75772 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-08505 | |||
![Immunocytochemistry/Immunofluorescence: MyoD Antibody (5.8A) [NB100-56511] - Human myoblasts were stained with MyoD1 antibody, diluted 1:200. ICC/IF image submitted by a verified customer review.](http://images.novusbio.com/fullsize/MyoD-Antibody-5-8A-Immunocytochemistry-Immunofluorescence-NB100-56511-img0008.jpg "Immunocytochemistry/Immunofluorescence: MyoD Antibody (5.8A) [NB100-56511] - Human myoblasts were stained with MyoD1 antibody, diluted 1:200. ICC/IF image submitted by a verified customer review.")
![<b>Biological Strategies Validation. </b>Western Blot: MyoD Antibody (5.8A) [NB100-56511] - Insuline-like growth factor (IGF1) antagonized the effects of miR-106a-5p on myogenic differentiation in C2C12. All data were collected from C2C12 myotubes 5 d post differentiation. Western-blot analysis of myogenic regulatory factors (MyoD, MyoG, MyHC) in cells; (H) The statistical results of Figure 3G. Data were presented as mean +/- SEM. n = 3 per group. * p < 0.05, ** p < 0.01. Image collected and cropped by CiteAb from the following publication (//www.mdpi.com/2073-4425/9/7/333/htm) licensed under a CC-BY license. anti-MyoG (catalog# NB100-56510)](http://images.novusbio.com/fullsize/MyoD-Antibody-5-8A-Western-Blot-NB100-56511-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: MyoD Antibody (5.8A) [NB100-56511] - Cell Lines Tested: mouse skeletal muscle-derived primary cell population Test Sample Preparation: mouse skeletal muscle digested by collagenase type 2 System: Super Sensitive High Resolution Confocal Laser Microscope (LSM880 with Airyscan) Excitation Wavelength: 488nm Emission Filter: 562 nm. Image using the DyLight 488 form of this antibody. ICC/IF image submitted by a verified customer review.](http://images.novusbio.com/fullsize/MyoD-Antibody-5-8A-Immunocytochemistry-Immunofluorescence-NB100-56511-img0007.jpg)
![Western Blot: MyoD Antibody (5.8A) [NB100-56511] - Analysis for MyoD expression in various small round cell tumor lines using 1 ug/mL anti-MyoD mAb. The antibody only reacts with a band of approx. 45 kDa in the rhabdomyosarcoma cell line (Rh30, lane 1) but was negative against the primitive neuroectodermal (PFSK-1A, lane 2), lymphoma (EB2, lane 3), neuroblastoma (SK-N-SH, lane 4), and Ewing's sarcoma (SJSA-1, lane 5) cell lines.](http://images.novusbio.com/fullsize/MyoD-Antibody-5-8A-Western-Blot-NB100-56511-img0006.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: MyoD Antibody (5.8A) [NB100-56511] - MiR-106a-5p inhibited the myogenic differentiation of C2C12 myoblasts. Western blot analyzed for MyoD, MyoG, MyHC proteins 5 d post differentiation. Image collected and cropped by CiteAb from the following publication (//www.mdpi.com/2073-4425/9/7/333/htm) licensed under a CC-BY license. anti-MyoG (catalog# NB100-56510)](http://images.novusbio.com/fullsize/MyoD-Antibody-5-8A-Western-Blot-NB100-56511-img0010.jpg)
![Immunohistochemistry-Frozen: MyoD Antibody (5.8A) [NB100-56511] - Clone 5.8A antibody in human tissues. A. Rhabdomyosaroma (nuclei are stained), B. Lymphoma (staining is absent)](http://images.novusbio.com/fullsize/MyoD-Antibody-5-8A-Immunohistochemistry-NB100-56511-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: MyoD Antibody (5.8A) [NB100-56511] - RD cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-MyoD1 (5.8A) NB100-56511 at a 1:200 dilution overnight at 4C and detected with and anti-mouse DyLight 488 (Green) at a 1:500 dilution. Actin was counterstained with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/MyoD-Antibody-5-8A-Immunocytochemistry-Immunofluorescence-NB100-56511-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: MyoD Antibody [NBP1-54153] - RD cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton X-100. The cells were incubated with anti-MyoD at 5 ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/MyoD-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-54153-img0005.jpg "Immunocytochemistry/Immunofluorescence: MyoD Antibody [NBP1-54153] - RD cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton X-100. The cells were incubated with anti-MyoD at 5 ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.")
![Western Blot: MyoD1 Antibody [NBP1-54153] - Detection of MYOD1 in RH30 cells.](http://images.novusbio.com/fullsize/MyoD1-Antibody-Western-Blot-NBP1-54153-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: MyoD Antibody [NBP1-54153] - Staing of MYOD1 in RD cells with FITC (green). Nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/MyoD1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-54153-img0003.jpg)
![Immunohistochemistry-Paraffin: MyoD Antibody (SPM427) [NBP2-32882] - Formalin-fixed, paraffin-embedded human Rhabdomyosarcoma stained with MyoD Antibody (SPM427).](http://images.novusbio.com/fullsize/MyoD-Antibody-SPM427-Immunohistochemistry-Paraffin-NBP2-32882-img0003.jpg "Immunohistochemistry-Paraffin: MyoD Antibody (SPM427) [NBP2-32882] - Formalin-fixed, paraffin-embedded human Rhabdomyosarcoma stained with MyoD Antibody (SPM427).")
![Immunocytochemistry/ Immunofluorescence: MyoD Antibody (5.2F) [NBP3-30815] - Figure 4. MyoD immunostaining of nuclei in MyoD transfected cells induced to undergo muscle cell differentiation.](http://images.novusbio.com/fullsize/nbp3-30815_mouse-myod-mab-5-2f-811202420592116.jpg "Immunocytochemistry/ Immunofluorescence: MyoD Antibody (5.2F) [NBP3-30815] - Figure 4. MyoD immunostaining of nuclei in MyoD transfected cells induced to undergo muscle cell differentiation.")
![Immunocytochemistry/ Immunofluorescence: MyoD Antibody (5.2F) [NBP3-30815] - Figure 3. MyoD immunostaining of nuclei in MyoD transfected cells induced to undergo muscle cell differentiation.](http://images.novusbio.com/fullsize/nbp3-30815_mouse-myod-mab-5-2f-811202420594212.jpg)
![Immunocytochemistry/ Immunofluorescence: MyoD Antibody (5.2F) [NBP3-30815] - Figure 2. MyoD immunostaining of nuclei in MyoD transfected cells induced to undergo muscle cell differentiation.](http://images.novusbio.com/fullsize/nbp3-30815_mouse-myod-mab-5-2f-81120242059212.jpg)
![Western Blot: MyoD Antibody (5.2F) [NBP3-30815] - Left: Immunohistochemical staining using MyoD antibody on formalin fixed, paraffin embedded human rhabdomyosarcoma. Right: Western blot using 60 ng of purified MyoD protein with MyoD antibody at 2 ug/ml and detected using goat anti-mouse HRP and visualized with Pierce West-Femto substrate.](http://images.novusbio.com/fullsize/nbp3-30815_mouse-myod-mab-5-2f-811202420592184.jpg)
![Western Blot: MyoD Antibody (HL1372) - Azide and BSA Free [NBP3-25573] - Various tissue extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with MyoD1 antibody [HL1372] (NBP3-25573) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25573_rabbit-myod-mab-hl1372-azide-and-bsa-free-212024939119.jpg "Western Blot: MyoD Antibody (HL1372) - Azide and BSA Free [NBP3-25573] - Various tissue extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with MyoD1 antibody [HL1372] (NBP3-25573) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: MyoD Antibody (HL1372) - Azide and BSA Free [NBP3-25573] - MyoD1 antibody [HL1372] detects MyoD1 protein at nucleus by immunofluorescent analysis. Sample: RMS-13 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: MyoD1 stained by MyoD1 antibody [HL1372] (NBP3-25573) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.](http://images.novusbio.com/fullsize/nbp3-25573_rabbit-myod-mab-hl1372-azide-and-bsa-free-2120249383179.jpg)
![Immunohistochemistry-Paraffin: MyoD Antibody (HL1372) - Azide and BSA Free [NBP3-25573] - MyoD1 antibody [HL1372] detects MyoD1 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse E13.5 embryo. Green: MyoD1 stained by MyoD1 antibody [HL1372] (NBP3-25573) diluted at 1:100. Red: SOX2, a nucleus marker, stained by SOX2 antibody [GT1352] (NBP2-19306) diluted at 1:250. Blue: Fluoroshield with DAPI . Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25573_rabbit-myod-mab-hl1372-azide-and-bsa-free-212024938313.jpg)
![Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with MyoD antibody [HL1372] (NBP3-25573) diluted at 1:15000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25573_rabbit-myod-mab-hl1372-azide-and-bsa-free-western-blot-1411202518243310.jpg)
![<b>Orthogonal Strategies Validation. </b>Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with MyoD antibody [HL1372] (NBP3-25573) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-25573_rabbit-myod-mab-hl1372-azide-and-bsa-free-western-blot-1411202518334516.jpg)
![ELISA: Human MyoD ELISA Kit (Colorimetric) [NBP3-39525] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-39525_human-myod-elisa-kit-colorimetric-2110202415273848.png "ELISA: Human MyoD ELISA Kit (Colorimetric) [NBP3-39525] - Standard Curve Reference")
![Immunohistochemistry-Paraffin: MyoD1 Antibody (MYD712) [NBP2-32883] - Formalin-fixed, paraffin-embedded human rhabdomyosarcoma stained with MyoD1 MAb (MYD712)](http://images.novusbio.com/fullsize/MyoD1-Antibody-MYD712-Immunohistochemistry-Paraffin-NBP2-32883-img0001.jpg "Immunohistochemistry-Paraffin: MyoD1 Antibody (MYD712) [NBP2-32883] - Formalin-fixed, paraffin-embedded human rhabdomyosarcoma stained with MyoD1 MAb (MYD712)")
![Immunohistochemistry-Paraffin: MyoD Antibody (MYOD1/2075R) [NBP2-75769] - Formalin-fixed, paraffin-embedded human rhabdomyosarcoma stained with MyoD Recombinant Rabbit Monoclonal Antibody (MYOD1/2075R).](http://images.novusbio.com/fullsize/MyoD-Antibody-MYOD1-2075R-Immunohistochemistry-Paraffin-NBP2-75769-img0005.jpg "Immunohistochemistry-Paraffin: MyoD Antibody (MYOD1/2075R) [NBP2-75769] - Formalin-fixed, paraffin-embedded human rhabdomyosarcoma stained with MyoD Recombinant Rabbit Monoclonal Antibody (MYOD1/2075R).")
![Immunohistochemistry-Paraffin: MyoD Antibody (MYOD1/2075R) [NBP2-75769] - Formalin-fixed, paraffin-embedded human Rhabdomyosarcoma stained with MyoD1 Mouse Monoclonal Antibody (MYOD1/2075R).](http://images.novusbio.com/fullsize/MyoD-Antibody-MYOD1-2075R-Immunohistochemistry-Paraffin-NBP2-75769-img0001.jpg)
![Immunohistochemistry-Paraffin: MyoD Antibody (MYOD1/2075R) [NBP2-75769] - Formalin-fixed, paraffin-embedded human Rhabdomyosarcoma stained with MyoD Antibody (MYOD1/2075R).](http://images.novusbio.com/fullsize/MyoD-Antibody-MYOD1-2075R-Immunohistochemistry-Paraffin-NBP2-75769-img0003.jpg)
![Protein Array: MyoD Antibody (MYOD1/2075R) [NBP2-75769] - Analysis of Protein Array containing more than 19,000 full-length human proteins using MyoD Antibody (MYOD1/2075R). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (Monoclonal Antibody) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target, if the Monoclonal Antibody has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/MyoD-Antibody-MYOD1-2075R-Protein-Array-NBP2-75769-img0004.jpg)
![Western Blot: MyoD Antibody (JE60-47) [NBP3-32630] - Western blot analysis of MyoD on different lysates with Rabbit anti-MyoD antibody (NBP3-32630) at 1/1,000 dilution.<br/><br/> Lane 1: RD cell lysate<br/> Lane 2: HEK-293 cell lysate (negative)<br/> Lane 3: HeLa cell lysate (negative)<br/> Lane 4: RD cell lysate<br/><br/> Lysates/proteins at 30 ug/Lane.<br/><br/> Predicted band size: 35 kDa<br/> Observed band size: 45 kDa<br/><br/> Exposure time: 10 seconds; ECL;<br/><br/> 4-20% SDS-PAGE gel.<br/><br/> Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32630) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32630_rabbit-myod-mab-je60-47-278202416285250.jpg "Western Blot: MyoD Antibody (JE60-47) [NBP3-32630] - Western blot analysis of MyoD on different lysates with Rabbit anti-MyoD antibody (NBP3-32630) at 1/1,000 dilution.<br/><br/> Lane 1: RD cell lysate<br/> Lane 2: HEK-293 cell lysate (negative)<br/> Lane 3: HeLa cell lysate (negative)<br/> Lane 4: RD cell lysate<br/><br/> Lysates/proteins at 30 ug/Lane.<br/><br/> Predicted band size: 35 kDa<br/> Observed band size: 45 kDa<br/><br/> Exposure time: 10 seconds; ECL;<br/><br/> 4-20% SDS-PAGE gel.<br/><br/> Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32630) at 1/1,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/ Immunofluorescence: MyoD Antibody (JE60-47) [NBP3-32630] - Immunocytochemistry analysis of RD (positive) and HEK-293 (negative) labeling MyoD with Rabbit anti-MyoD antibody (NBP3-32630) at 1/100 dilution.<br/><br/> Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-MyoD antibody (NBP3-32630) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br/><br/> Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32630_rabbit-myod-mab-je60-47-2920241192395.jpg)
![Western Blot: MyoD Antibody (RM369) [NBP2-77430] - Western Blot of mouse skeletal muscle tissue lysate using RM369 at a 1:2000 dilution.](http://images.novusbio.com/fullsize/nbp2-77430_rabbit-monoclonal-myod-antibody-rm369-24620241332459.png "Western Blot: MyoD Antibody (RM369) [NBP2-77430] - Western Blot of mouse skeletal muscle tissue lysate using RM369 at a 1:2000 dilution.")
![Immunohistochemistry-Paraffin: MyoD Antibody (RM369) [NBP2-77430] - Mouse skeletal muscle tissue lysate using anti-MyoD1 rabbit monoclonal antibody (Clone RM369) at a 1:2000 dilution.](http://images.novusbio.com/fullsize/nbp2-77430_rabbit-monoclonal-myod-antibody-rm369-immunohistochemistry-paraffin-189202523374021.png)
![Immunohistochemistry-Paraffin: MyoD Antibody (rMYD712) [NBP3-07394] - Formalin-fixed, paraffin-embedded human Rhabdomyosarcoma stained with MyoD Mouse Recombinant Monoclonal Antibody (rMYD712).](http://images.novusbio.com/fullsize/MyoD-Antibody-rMYD712-Immunohistochemistry-Paraffin-NBP3-07394-img0002.jpg "Immunohistochemistry-Paraffin: MyoD Antibody (rMYD712) [NBP3-07394] - Formalin-fixed, paraffin-embedded human Rhabdomyosarcoma stained with MyoD Mouse Recombinant Monoclonal Antibody (rMYD712).")
![Protein Array: MyoD Antibody (rMYD712) [NBP3-07394] - Analysis of Protein Array containing more than 19,000 full-length human proteins using MyoD Mouse Recombinant Monoclonal Antibody (rMYD712).](http://images.novusbio.com/fullsize/MyoD-Antibody-rMYD712-Protein-Array-NBP3-07394-img0001.jpg)
