Products Related to Gene-ID: 3976

CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox"> CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." alt="LIF was detected in immersion fixed paraffin-embedded sections of human lung array using Goat Anti-Human LIF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-250-NA) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox"> CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

7734-LF) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-depend-ent manner (orange line), as measured by Resazurin (AR002). Proliferation elicited by 3 ng/mL Recombinant Human LIF is neutralized (green line) by increasing concentrations of Goat Anti-Human LIF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-250-NA). The ND₅₀ is typically 0.04-0.08 µg/mL." class="big_lightbox"> 7734-LF) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-depend-ent manner (orange line), as measured by Resazurin (AR002). Proliferation elicited by 3 ng/mL Recombinant Human LIF is neutralized (green line) by increasing concentrations of Goat Anti-Human LIF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-250-NA). The ND₅₀ is typically 0.04-0.08 µg/mL." class="big_thumb" />

FAB2155P) and APC-conjugated Mouse Anti-Human/Mouse SSEA-4 APC-conjugated Monoclonal Antibody (Catalog # FAB1435A). ESCs continue to express the pluripotency marker SSEA-1 and lack expression of SSEA-4, a negative marker for mouse ESCs. Quadrants were set based on samples stained with isotype control antibodies." class="big_lightbox"> FAB2155P) and APC-conjugated Mouse Anti-Human/Mouse SSEA-4 APC-conjugated Monoclonal Antibody (Catalog # FAB1435A). ESCs continue to express the pluripotency marker SSEA-1 and lack expression of SSEA-4, a negative marker for mouse ESCs. Quadrants were set based on samples stained with isotype control antibodies." alt=" Recombinant Mouse LIF Promotes the Expansion of Mouse Embryonic Stem Cells. D3 mouse ESCs were cultured with Recombinant Mouse LIF (10 ng/mL). After 15 days, and following multiple rounds of passaging, the ESCs were analyzed for pluripotency by staining with PE-conjugated Mouse Anti-Human/Mouse SSEA-1 PE-conjugated Monoclonal Antibody (Catalog # FAB2155P) and APC-conjugated Mouse Anti-Human/Mouse SSEA-4 APC-conjugated Monoclonal Antibody (Catalog # FAB1435A). ESCs continue to express the pluripotency marker SSEA-1 and lack expression of SSEA-4, a negative marker for mouse ESCs. Quadrants were set based on samples stained with isotype control antibodies." class="big_thumb" />

AF2018) followed by the Donkey Anti-Goat IgG NorthernLights™ NL493-conjugated Polyclonal Antibody (Catalog # NL003). The cell nuclei were stained with DAPI (blue)." class="big_lightbox"> AF2018) followed by the Donkey Anti-Goat IgG NorthernLights™ NL493-conjugated Polyclonal Antibody (Catalog # NL003). The cell nuclei were stained with DAPI (blue)." class="big_thumb" />

8878-LF) stimulates IL-6 secretion in the M1 mouse myeloid leukemia cell line in a dose-dependent manner (orange line) as measured by the Mouse IL-6 Quantikine ELISA (Catalog # M6000B). IL-6 secretion elicited by 3 ng/mL Recombinant Mouse LIF is neutralized (green line) by increasing concentrations of Goat Anti-Mouse LIF Polyclonal Antibody (Catalog # AB-449-NA). The ND₅₀ is typically 80-480 ng/mL." class="big_lightbox"> 8878-LF) stimulates IL-6 secretion in the M1 mouse myeloid leukemia cell line in a dose-dependent manner (orange line) as measured by the Mouse IL-6 Quantikine ELISA (Catalog # M6000B). IL-6 secretion elicited by 3 ng/mL Recombinant Mouse LIF is neutralized (green line) by increasing concentrations of Goat Anti-Mouse LIF Polyclonal Antibody (Catalog # AB-449-NA). The ND₅₀ is typically 80-480 ng/mL." alt="Recombinant Mouse LIF (Catalog # 8878-LF) stimulates IL-6 secretion in the M1 mouse myeloid leukemia cell line in a dose-dependent manner (orange line) as measured by the Mouse IL-6 Quantikine ELISA (Catalog # M6000B). IL-6 secretion elicited by 3 ng/mL Recombinant Mouse LIF is neutralized (green line) by increasing concentrations of Goat Anti-Mouse LIF Polyclonal Antibody (Catalog # AB-449-NA). The ND₅₀ is typically 80-480 ng/mL." class="big_thumb" />

CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to sensory neurons in trigeminal ganglia. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_lightbox"> CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to sensory neurons in trigeminal ganglia. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_thumb" />

8878-LF) stimulates IL-6 secretion in the M1 mouse myeloid leukemia cell line in a dose-dependent manner (orange line) as measured by the Mouse IL-6 Quantikine ELISA (Catalog # M6000B). IL-6 secretion elicited by 3 ng/mL Recombinant Mouse LIF is neutralized (green line) by increasing concentrations of Goat Anti-Mouse LIF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF449). The ND₅₀ is typically 10-60 ng/mL." class="big_lightbox"> 8878-LF) stimulates IL-6 secretion in the M1 mouse myeloid leukemia cell line in a dose-dependent manner (orange line) as measured by the Mouse IL-6 Quantikine ELISA (Catalog # M6000B). IL-6 secretion elicited by 3 ng/mL Recombinant Mouse LIF is neutralized (green line) by increasing concentrations of Goat Anti-Mouse LIF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF449). The ND₅₀ is typically 10-60 ng/mL." alt="Recombinant Mouse LIF (Catalog # 8878-LF) stimulates IL-6 secretion in the M1 mouse myeloid leukemia cell line in a dose-dependent manner (orange line) as measured by the Mouse IL-6 Quantikine ELISA (Catalog # M6000B). IL-6 secretion elicited by 3 ng/mL Recombinant Mouse LIF is neutralized (green line) by increasing concentrations of Goat Anti-Mouse LIF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF449). The ND₅₀ is typically 10-60 ng/mL." class="big_thumb" />

7734-LF) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line) as measured by Resazurin (Catalog # AR002). Proliferation elicited by 0.5 ng/mL Recom-binant Human LIF is neutralized (green line) by increasing concentrations of Goat Anti-Human LIF Polyclonal Antibody (Catalog # AB-250-NA). The ND₅₀ is typically 0.1-0.4 µg/mL." class="big_lightbox"> 7734-LF) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line) as measured by Resazurin (Catalog # AR002). Proliferation elicited by 0.5 ng/mL Recom-binant Human LIF is neutralized (green line) by increasing concentrations of Goat Anti-Human LIF Polyclonal Antibody (Catalog # AB-250-NA). The ND₅₀ is typically 0.1-0.4 µg/mL." alt="Recombinant Human LIF (Catalog # 7734-LF) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line) as measured by Resazurin (Catalog # AR002). Proliferation elicited by 0.5 ng/mL Recom-binant Human LIF is neutralized (green line) by increasing concentrations of Goat Anti-Human LIF Polyclonal Antibody (Catalog # AB-250-NA). The ND₅₀ is typically 0.1-0.4 µg/mL." class="big_thumb" />