Products Related to Gene-ID: 3976

MAB002, open histogram) followed by APC-conjugated Goat anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules." class="big_lightbox"> MAB002, open histogram) followed by APC-conjugated Goat anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules." alt="HepG2 human hepatocellular carcinoma cell line was stained with Mouse Anti-Human LIF Monoclonal Antibody (Catalog # MAB2501, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram) followed by APC-conjugated Goat anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules." class="big_thumb" />

7734-LF) induce TF-1 human erythroleukemic cell proliferation. The ED₅₀ for this effect is 0.02-0.12 ng/mL. The similarity in activity highlights that the biotinylated protein is fully functional." class="big_lightbox"> 7734-LF) induce TF-1 human erythroleukemic cell proliferation. The ED₅₀ for this effect is 0.02-0.12 ng/mL. The similarity in activity highlights that the biotinylated protein is fully functional." alt="Both Recombinant Biotinylated Human LIF (Catalog # BT7734) and unlabeled Recombinant Human LIF (Catalog # 7734-LF) induce TF-1 human erythroleukemic cell proliferation. The ED₅₀ for this effect is 0.02-0.12 ng/mL. The similarity in activity highlights that the biotinylated protein is fully functional." class="big_thumb" />

DY990). Goat Anti-Human LIF Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF250) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." class="big_lightbox"> DY990). Goat Anti-Human LIF Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF250) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." alt="Recombinant Human LIF protein was serially diluted 2-fold and captured by Mouse Anti-Human LIF Monoclonal Antibody (Catalog # MAB2502) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human LIF Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF250) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." class="big_thumb" />

DY990). Goat Anti-Human LIF Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF250) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> DY990). Goat Anti-Human LIF Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF250) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />