| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-45056 | |||
| Azide and BSA Free | NBP2-34601-0.1mg | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Biotin | NBP1-97291 | |||
| FITC | NBP1-97272 | |||
| HRP | NBP1-97296 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Pre-adsorbed | NBP1-75192 | |||
| Pre-adsorbed | NBP1-75193 | |||
| Pre-adsorbed | NBP1-75189JF549 | |||
| Pre-adsorbed | NBP1-75189JF646 | |||
![Immunohistochemistry-Paraffin: IgM Antibody (DA4-4 (same as SA-DA4 or HB57)) [NBP2-34252] - Formalin-fixed, paraffin-embedded human tonsil stained with IgM MAb (DA4-4)](http://images.novusbio.com/fullsize/IgM-Antibody-DA4-4-same-as-SA-DA4-or-HB57-Immunohistochemistry-Paraffin-NBP2-34252-img0001.jpg "Immunohistochemistry-Paraffin: IgM Antibody (DA4-4 (same as SA-DA4 or HB57)) [NBP2-34252] - Formalin-fixed, paraffin-embedded human tonsil stained with IgM MAb (DA4-4)")
![SDS-Page: Porcine IgM Isotype Control [NBP1-96788] - Lane 1: Swine IgM, 1.0 ug, Non-reduced. Lane 2: Swine IgM, 5.0 ug, Non-reduced. Lane 3: Swine IgM, 10.0 ug, Non-reduced. M: Opal Pre-stained Marker. Lane 4: Swine IgM, 1.0 ug, Reduced. Lane 5: Swine IgM, 5.0 ug, Reduced. Lane 6: Swine IgM, 10.0 ug, Reduced. Predicted/Observed size - Non-Reduced: 900 kDa, Reduced - 75, 25 kDa.](http://images.novusbio.com/fullsize/Porcine-IgM-Isotype-Control-SDS-Page-NBP1-96788-img0001.jpg "SDS-Page: Porcine IgM Isotype Control [NBP1-96788] - Lane 1: Swine IgM, 1.0 ug, Non-reduced. Lane 2: Swine IgM, 5.0 ug, Non-reduced. Lane 3: Swine IgM, 10.0 ug, Non-reduced. M: Opal Pre-stained Marker. Lane 4: Swine IgM, 1.0 ug, Reduced. Lane 5: Swine IgM, 5.0 ug, Reduced. Lane 6: Swine IgM, 10.0 ug, Reduced. Predicted/Observed size - Non-Reduced: 900 kDa, Reduced - 75, 25 kDa.")
![SDS-Page: Sheep IgM Isotype Control [NBP1-96774] - Lane 1: Sheep IgM, Non-Reduced. Lane 2: Sheep IgM, Reduced. Load: 1.0 ug per lane.](http://images.novusbio.com/fullsize/Sheep-IgM-Isotype-Control-SDS-Page-NBP1-96774-img0001.jpg "SDS-Page: Sheep IgM Isotype Control [NBP1-96774] - Lane 1: Sheep IgM, Non-Reduced. Lane 2: Sheep IgM, Reduced. Load: 1.0 ug per lane.")
![SDS-Page: Human IgM Heavy Chain Isotype Control [NBP1-96772] - SDS PAGE Results of Human IgM Heavy Chain isotype control. Lane 1: Human IgM Heavy Chain isotype control reduced [5ug]. Lane 2: Opal Prestained Molecular Weight Marker. Lane 3: Human IgM Heavy Chain isotype control non-reduced [5ug]. 4-20% gel, Coomassie stained. Bands at expected MW.](http://images.novusbio.com/fullsize/Human-IgM-Heavy-Chain-Isotype-Control-SDS-Page-NBP1-96772-img0001.jpg "SDS-Page: Human IgM Heavy Chain Isotype Control [NBP1-96772] - SDS PAGE Results of Human IgM Heavy Chain isotype control. Lane 1: Human IgM Heavy Chain isotype control reduced [5ug]. Lane 2: Opal Prestained Molecular Weight Marker. Lane 3: Human IgM Heavy Chain isotype control non-reduced [5ug]. 4-20% gel, Coomassie stained. Bands at expected MW.")
![Dot Blot: Rabbit anti-Mouse IgM Heavy Chain Secondary Antibody [NBP1-72789] - Mouse IgM. Load: Lane 1 - 200 ng Lane 2 - 66.7 ng Lane 3 - 22.2 ng Lane 4 - 7.41 ng Lane 5 - 2.47 ng.](http://images.novusbio.com/fullsize/Rabbit-anti-Mouse-IgM-Heavy-Chain-Secondary-Antibody-Dot-Blot-NBP1-72789-img0001.jpg "Dot Blot: Rabbit anti-Mouse IgM Heavy Chain Secondary Antibody [NBP1-72789] - Mouse IgM. Load: Lane 1 - 200 ng Lane 2 - 66.7 ng Lane 3 - 22.2 ng Lane 4 - 7.41 ng Lane 5 - 2.47 ng.")
![ELISA: Rabbit anti-Mouse IgM Heavy Chain Secondary Antibody [NBP1-72789] - ELISA Results of Rabbit anti-Mouse IgM Heavy Chain Secondary antibody tested against purified Mouse IgM. Each well was coated in duplicate with 10 ug of Mouse IgM. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% Fish Gel/PBS Blocking buffer, Goat Anti-Rabbit IgG mx10 HRP conjugated, and TMB substrate.](http://images.novusbio.com/fullsize/Rabbit-anti-Mouse-IgM-Heavy-Chain-Secondary-Antibody-ELISA-NBP1-72789-img0004.jpg)
![Western Blot: Rabbit anti-Rat IgM Heavy Chain Secondary Antibody [NBP1-72790] - Lane 1: Rat IgG whole molecule. Lane 2: Rat IgG F(c) Fragment. Lane 3: Rat IgG Fab Fragment. Lane 4: Rat IgM Whole Molecule. Lane 5: Rat Serum. All samples were reduced. Load: 50 ng per lane. Block: incubated with blocking buffer for 30 min at RT. Primary Antibody: Anti-Rat IgM (mu chain) (RABBIT) Antibody 1:2,000 for 60 min at RT. Secondary Antibody: Anti-Rabbit IgG (GOAT) Peroxidase Conjugated Antibody 1:40,000 in incubated with blocking buffer for 30 min at RT. Predicted/Observed Size: 25 and 55 kDa for Rat IgG and Serum, 25 kDa for F(c) and Fab, 78 and 25 kDa for IgM. Rat F(c) migrates slightly higher.](http://images.novusbio.com/fullsize/Rabbit-anti-Rat-IgM-Secondary-Antibody-[Unconjugated]-Western-Blot-NBP1-72790-img0004.jpg "Western Blot: Rabbit anti-Rat IgM Heavy Chain Secondary Antibody [NBP1-72790] - Lane 1: Rat IgG whole molecule. Lane 2: Rat IgG F(c) Fragment. Lane 3: Rat IgG Fab Fragment. Lane 4: Rat IgM Whole Molecule. Lane 5: Rat Serum. All samples were reduced. Load: 50 ng per lane. Block: incubated with blocking buffer for 30 min at RT. Primary Antibody: Anti-Rat IgM (mu chain) (RABBIT) Antibody 1:2,000 for 60 min at RT. Secondary Antibody: Anti-Rabbit IgG (GOAT) Peroxidase Conjugated Antibody 1:40,000 in incubated with blocking buffer for 30 min at RT. Predicted/Observed Size: 25 and 55 kDa for Rat IgG and Serum, 25 kDa for F(c) and Fab, 78 and 25 kDa for IgM. Rat F(c) migrates slightly higher.")
