DR555RB) and counterstained with DAPI (blue; Lunaphore Catalog #
DR100). Specific staining was localized to the membrane. Protocol available in
COMET™ Panel Builder.​​" class="big_lightbox">

DR555RB) and counterstained with DAPI (blue; Lunaphore Catalog #
DR100). Specific staining was localized to the membrane. Protocol available in
COMET™ Panel Builder.​​" alt="IgM was detected in immersion fixed paraffin-embedded sections of human Non-Hodgkins Lymphoma using Rabbit Anti-Human IgM, Monoclonal Antibody (Catalog #MAB9435) at 0.2ug/mL at 37° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ Plus 555 Goat anti-Rabbit IgG Secondary Antibody at 1:100 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog #
DR555RB) and counterstained with DAPI (blue; Lunaphore Catalog #
DR100). Specific staining was localized to the membrane. Protocol available in
COMET™ Panel Builder.​​" class="big_thumb" />
DR555RB) and counterstained with DAPI (blue; Lunaphore Catalog #
DR100). Specific staining was localized to the membrane. Protocol available in
COMET™ Panel Builder." class="big_lightbox">

DR555RB) and counterstained with DAPI (blue; Lunaphore Catalog #
DR100). Specific staining was localized to the membrane. Protocol available in
COMET™ Panel Builder." class="big_thumb" />
DR555RB) and counterstained with DAPI (blue; Lunaphore Catalog #
DR100). Specific staining was localized to the membrane. Protocol available in
COMET™ Panel Builder.​" class="big_lightbox">

DR555RB) and counterstained with DAPI (blue; Lunaphore Catalog #
DR100). Specific staining was localized to the membrane. Protocol available in
COMET™ Panel Builder.​" class="big_thumb" />
HAF008). A specific band was detected for IgM at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using
Immunoblot Buffer Group 1." class="big_lightbox">

HAF008). A specific band was detected for IgM at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using
Immunoblot Buffer Group 1." class="big_thumb" />
DY990). Rabbit Anti-Human IgM Monoclonal Antibody (
MAB94351) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (
DY998) followed by Substrate Solution (
DY999) and stopping the enzymatic reaction with Stop Solution (
DY994)." class="big_lightbox">

DY990). Rabbit Anti-Human IgM Monoclonal Antibody (
MAB94351) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (
DY998) followed by Substrate Solution (
DY999) and stopping the enzymatic reaction with Stop Solution (
DY994)." class="big_thumb" />
HAF008) or the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog #
VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog #
VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the membrane of B cells. View our protocol for
Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox">

HAF008) or the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog #
VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog #
VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the membrane of B cells. View our protocol for
Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />