DY990). Sheep Anti-Human Cyr61/CCN1 Antigen Affinity-purified Polyclonal Antibody (Catalog #
AF6009) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog #
DY998) followed by Substrate Solution (Catalog #
DY999) and stopping the enzymatic reaction with Stop Solution (Catalog #
DY994)." class="big_lightbox">
DY990). Sheep Anti-Human Cyr61/CCN1 Antigen Affinity-purified Polyclonal Antibody (Catalog #
AF6009) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog #
DY998) followed by Substrate Solution (Catalog #
DY999) and stopping the enzymatic reaction with Stop Solution (Catalog #
DY994)." alt="Recombinant Human Cyr61/CCN1 protein was serially diluted 2-fold and captured by Rat Anti-Human Cyr61/CCN1 Monoclonal Antibody (Catalog # MAB40551) coated on a Clear Polystyrene Microplate (Catalog #
DY990). Sheep Anti-Human Cyr61/CCN1 Antigen Affinity-purified Polyclonal Antibody (Catalog #
AF6009) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog #
DY998) followed by Substrate Solution (Catalog #
DY999) and stopping the enzymatic reaction with Stop Solution (Catalog #
DY994)." class="big_thumb" />
4055-CR) was serially diluted and captured by Rat Anti-Human Cyr61/CCN1 Monoclonal Antibody (Catalog # MAB40551) coated on a Clear Polystyrene Microplate (Catalog #
DY990). Sheep Anti-Human Cyr61/CCN1 Antigen Affinity-purified Polyclonal Antibody (Catalog #
AF6009) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog #
DY998)" class="big_lightbox">
4055-CR) was serially diluted and captured by Rat Anti-Human Cyr61/CCN1 Monoclonal Antibody (Catalog # MAB40551) coated on a Clear Polystyrene Microplate (Catalog #
DY990). Sheep Anti-Human Cyr61/CCN1 Antigen Affinity-purified Polyclonal Antibody (Catalog #
AF6009) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog #
DY998)" class="big_thumb" />
HAF016). A specific band was detected for Cyr61/CCN1 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of mouse spleenocyte cell, RAW 264.7 mouse monocyte/macrophage cell line, NIH-3T3 mouse embryonic fibroblast cell line, and A20 mouse B cell lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Cyr61/CCN1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4055) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Cyr61/CCN1 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />
HAF016). A specific band was detected for Cyr61/CCN1 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2." alt="Western blot shows lysates of A549 human lung carcinoma cell line and SK-BR-3 human breast cancer cell line. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human Cyr61/CCN1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6009) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Cyr61/CCN1 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2." class="big_thumb" />
CTS017) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." alt="Cyr61/CCN1 was detected in perfusion fixed frozen sections of mouse trigeminal ganglia using 25 µg/mL Rat Anti-Mouse Cyr61/CCN1 Monoclonal Antibody (Catalog # MAB4864) overnight at 4 °C. Tissue was stained with the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_thumb" />
DY990). Sheep Anti-Human Cyr61/CCN1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6009) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." alt="Recombinant Human Cyr61/CCN1 protein was serially diluted 2-fold and captured by Rat Anti-Human Cyr61/CCN1 Monoclonal Antibody (Catalog # MAB40551) coated on a Clear Polystyrene Microplate (Catalog # DY990). Sheep Anti-Human Cyr61/CCN1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6009) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." class="big_thumb" />
![<b>Biological Strategies Validation. </b>Western Blot: Cyr61/CCN1 Antibody [NB100-356] - Total protein from Human liver, spleen and HepG2 cells was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/mL anti-CYR61 in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence. Note the low expression level of CYR61 in HepG2 cells.](http://images.novusbio.com/fullsize/Cyr61-CCN1-Antibody-Western-Blot-NB100-356-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: Cyr61/CCN1 Antibody [NB100-356] - MCF7 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-Cyr61/CCN1 (NB100-356) at 1:200 overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at 1:500. Alpha tubulin was used as a co-stain at 1:1000 and detected with an anti-mouse DyLight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize/Cyr61-CCN1-Antibody-Immunocytochemistry-Immunofluorescence-NB100-356-img0005.jpg)
![Western Blot: Cyr61/CCN1 Antibody [NB100-356] - Detection of cleaved CYR61 in MDA-MB-231 cell lysate using NB 100-356. Photo courtesy of Dr. Lupu's Lab, Northwestern University. Image by Dr. Ingrid Espinoza.](http://images.novusbio.com/fullsize/Cyr61-CCN1-Antibody-Western-Blot-NB100-356-img0004.jpg)
![Immunohistochemistry: Cyr61/CCN1 Antibody [NB100-356] - Human endometrium. Photo courtesy of Dr. rer. nat. Isabella Gashaw, University Duisburg-Essen.](http://images.novusbio.com/fullsize/Cyr61-CCN1-Antibody-Immunohistochemistry-NB100-356-img0001.jpg)
![Immunohistochemistry: Cyr61/CCN1 Antibody [NB100-356] - Human endometrium. Photo courtesy of Dr. rer. nat. Isabella Gashaw, University Duisburg-Essen.](http://images.novusbio.com/fullsize/Cyr61-CCN1-Antibody-Immunohistochemistry-NB100-356-img0002.jpg)
![Simple Western: Cyr61/CCN1 Antibody [NB100-356] - Image shows a specific band for CYR61 in 0.5 mg/mL of MCF-7 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/Cyr61-CCN1-Antibody-Simple-Western-NB100-356-img0003.jpg)
![Immunocytochemistry/ Immunofluorescence: Cyr61/CCN1 Antibody - BSA Free [NB100-356] - P4 inhibits E2-dependent cell proliferation & angiogenesis in ectopic lesions.Sections of the ectopic lesions collected from E2 or E2 plus P4-treated recipients (D16, n = 6) were subjected to histological examination. (A) Representative images (20X) showing H&E & Trichrome staining, or IHC analysis using antibody against myofibroblast biomarker alpha SMA, uterine epithelial biomarker KRT11, or uterine stromal biomarker VIM, respectively. (B) Representative images (20X) showing IHC analysis using antibodies against cell proliferation biomarker KI67, smooth muscle biomarker alpha SMA, endothelial cells CD31, or an angiogenetic regulator CCN1, respectively. The numbers of KI67-positive cells, the perimeters of the supporting blood vessels, & the immunostaining intensities of CCN1 & CD31 were analyzed by ImageJ software. The numerical values were analyzed by One-way ANOVA followed by Dunnett’s post hoc test & expressed as mean ± SEM. Statistical significance is defined as #: p < 0.05, *: p<0.01. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0165347), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-356_rabbit-polyclonal-cyr61-ccn1-antibody-310202415525265.jpg)
CTS019) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of muscle cells. View our protocol for 
HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_thumb" />
![N/A Cyr61/CCN1 [HRP]](http://images.novusbio.com/fullsize/elisa/DATA_Cyr61_DCYR10_ELISA_649.jpg "N/A Cyr61/CCN1 [HRP]")
![N/A Cyr61/CCN1 [HRP]](http://images.novusbio.com/fullsize/elisa/Cyr61_DCYR10_ELISA_437.jpg)
![Western Blot: Cyr61/CCN1 Antibody (3A7.1B8) [NBP2-36490] - WB analysis of (A) 12kDa Partial Recombinant Cyr61/CCN1 protein and (B) Human Liver lysate using Cyr61/CCN1 antibody (clone 3A7.1B8) at 4ug/ml concentration.](http://images.novusbio.com/fullsize/Cyr61-CCN1-Antibody-3A7-1B8-Western-Blot-NBP2-36490-img0004.jpg "Western Blot: Cyr61/CCN1 Antibody (3A7.1B8) [NBP2-36490] - WB analysis of (A) 12kDa Partial Recombinant Cyr61/CCN1 protein and (B) Human Liver lysate using Cyr61/CCN1 antibody (clone 3A7.1B8) at 4ug/ml concentration.")
![Immunohistochemistry-Paraffin: Cyr61/CCN1 Antibody (3A7.1B8) [NBP2-36490] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human liver using Cyr61/CCN1 antibody (clone 3A7.1B8) at 5 ug/ml concentration. The hepatocytes generated a specific cytoplasmic staining with no signal in the cellular nuclei or the sinusoids.](http://images.novusbio.com/fullsize/Cyr61-CCN1-Antibody-3A7-1B8-Immunohistochemistry-Paraffin-NBP2-36490-img0003.jpg)
![Immunohistochemistry-Paraffin: Cyr61/CCN1 Antibody (3A7.1B8) [NBP2-36490] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human hepatocellular carcinoma using Cyr61/CCN1 antibody (clone 3A7.1B8) at 5 ug/ml concentration. The cancer cells generated a specific cytoplasmic staining with some staining in the inter-cellular spaces but no signal in the cellular nuclei, tumor stroma or the RBCs.](http://images.novusbio.com/fullsize/Cyr61-CCN1-Antibody-3A7-1B8-Immunohistochemistry-Paraffin-NBP2-36490-img0001.jpg)
![Immunohistochemistry-Paraffin: Cyr61/CCN1 Antibody (3A7.1B8) [NBP2-36490] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human Renal Cell Carcinoma using Cyr61/CCN1 antibody (clone 3A7.1B8) at 5 ug/ml concentration. The cancer cells generated a specific cytoplasmic staining with some staining in the inter-cellular spaces but no signal in the cellular nuclei, tumor stroma or the RBCs.](http://images.novusbio.com/fullsize/Cyr61-CCN1-Antibody-3A7-1B8-Immunohistochemistry-Paraffin-NBP2-36490-img0002.jpg)
HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_thumb" />
MAB40551) coated on a Clear Polystyrene Microplate (Catalog # 
4055-CR) was serially diluted and captured by Rat Anti-Human Cyr61/CCN1 Monoclonal Antibody (Catalog # ![SDS-Page: Human Cyr61/CCN1 Protein [NBP2-34944]](http://images.novusbio.com/fullsize/Human-Cyr61-CCN1-Protein-SDS-Page-NBP2-34944-img0001.jpg "SDS-Page: Human Cyr61/CCN1 Protein [NBP2-34944]")
![Western Blot: Cyr61/CCN1 Antibody (HL2144) - Azide and BSA Free [NBP3-25430] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with CYR61 antibody [HL2144] (NBP3-25430) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-25430_rabbit-cyr61-ccn1-mab-hl2144-azide-and-bsa-free-2120249415975.jpg "<b>Orthogonal Strategies Validation. </b>Western Blot: Cyr61/CCN1 Antibody (HL2144) - Azide and BSA Free [NBP3-25430] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with CYR61 antibody [HL2144] (NBP3-25430) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Cyr61/CCN1 Antibody (HL2144) - Azide and BSA Free [NBP3-25430] - CYR61 antibody [HL2144] detects CYR61 protein at secreted protein by immunofluorescent analysis. Sample: C2C12 cells were fixed in ice-cold MeOH for 5 min. Green: CYR61 stained by CYR61 antibody [HL2144] (NBP3-25430) diluted at 1:500. Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25430_rabbit-cyr61-ccn1-mab-hl2144-azide-and-bsa-free-212024941486.jpg)
![Western Blot: Cyr61/CCN1 Antibody (HL2144) - Azide and BSA Free [NBP3-25430] - Rat tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with CYR61 antibody [HL2144] (NBP3-25430) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25430_rabbit-cyr61-ccn1-mab-hl2144-azide-and-bsa-free-2120249351373.jpg)
![Immunohistochemistry-Paraffin: Cyr61/CCN1 Antibody (HL2144) - Azide and BSA Free [NBP3-25430] - CYR61 antibody [HL2144] detects CYR61 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human colon cancer. CYR61 stained by CYR61 antibody [HL2144] (NBP3-25430) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25430_rabbit-cyr61-ccn1-mab-hl2144-azide-and-bsa-free-2120249342777.jpg)
![Western Blot: Cyr61/CCN1 Antibody (HL2144) - Azide and BSA Free [NBP3-25430] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with CYR61 antibody [HL2144] (NBP3-25430) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/nbp3-25430_rabbit-cyr61-ccn1-mab-hl2144-azide-and-bsa-free-2120249383159.jpg)
![N/A Cyr61/CCN1 [Biotin]](http://images.novusbio.com/fullsize/elisa/DATA_Cyr61_DY4055_ELISA_2003.jpg "N/A Cyr61/CCN1 [Biotin]")
4055-CR) was serially diluted and captured by Rat Anti-Human Cyr61/CCN1 Monoclonal Antibody (Catalog # MAB40551) coated on a Clear Polystyrene Microplate (Catalog # 
