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F0101B). Quadrant markers were set based on control antibody staining (MAB002, data not shown). Staining was performed using our Staining Membrane-Associated Proteins protocol." class="big_lightbox"> F0101B). Quadrant markers were set based on control antibody staining (MAB002, data not shown). Staining was performed using our Staining Membrane-Associated Proteins protocol." alt="HEK293 human embryonic kidney cell line transfected with either (A) human TMEM119 or (B) irrelevant protein, and eGFP, was stained with Mouse anti-human TMEM119 monoclonal antibody (Catalog # MAB10313) followed by Allophycocyanin-conjugated anti-Mouse IgG Secondary Antibody (F0101B). Quadrant markers were set based on control antibody staining (MAB002, data not shown). Staining was performed using our Staining Membrane-Associated Proteins protocol." class="big_thumb" />

VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to gilal cells. Staining was performed using our IHC Staining with VisUCyte HRP Polymer Detection Reagents protocol." class="big_lightbox"> VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to gilal cells. Staining was performed using our IHC Staining with VisUCyte HRP Polymer Detection Reagents protocol." class="big_thumb" />

VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell membrane and cytoplasm in lymphocytes. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox"> VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell membrane and cytoplasm in lymphocytes. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." alt="TMEM119 was detected in immersion fixed paraffin-embedded sections of human lymph node using Rabbit Anti-Human TMEM119 Monoclonal Antibody (Catalog # MAB103131) at 1 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell membrane and cytoplasm in lymphocytes. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />

F0111). Quadrant markers were set based on Rabbit IgG Isotype Control (MAB1050, data not shown). Staining was performed using our Staining Membrane-associated Proteins protocol. " class="big_lightbox"> F0111). Quadrant markers were set based on Rabbit IgG Isotype Control (MAB1050, data not shown). Staining was performed using our Staining Membrane-associated Proteins protocol. " class="big_thumb" />