LYVE-1: Novus Biologicals

AF2125

LYVE-1 Antibody [Unconjugated]

(1 Review)

100 μg

AF2125

CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of endothelial cells in sinusoids. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_lightbox">

LYVE-1 was detected in perfusion fixed frozen sections of mouse liver using 15 µg/mL Goat Anti-Mouse LYVE-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2125) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; <a class=

CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of endothelial cells in sinusoids. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." alt="LYVE-1 was detected in perfusion fixed frozen sections of mouse liver using 15 µg/mL Goat Anti-Mouse LYVE-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2125) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of endothelial cells in sinusoids. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_thumb" />

HAF019). A specific band was detected for LYVE-1 at approximately 60-65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

Western blot shows lysates of mouse liver tissue and bEnd.3 mouse brain endothelial cell line. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse LYVE-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2125) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (<a class=

HAF019). A specific band was detected for LYVE-1 at approximately 60-65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

17D-204 causes viscerotropic abnormalities in the absence of IFN-gamma . H&E sections (a–c) antibody-stained frozen sections (b, d) of spleen (a, b) and liver (c, d) were presented. a Spleen sections from 17D-204-infected animals were indistinguishable at 4 dpi, whereas Angola71-infected animals display loss of white pulp and red pulp architecture and increase in infiltrating macrophages and neutrophils (inset) at 4 dpi. At 11 dpi, 17D-204 infected AGB6 has increased immune infiltration and extramedullary hematopoiesis (inset) but not AB6 mice. b YFV antigen can be detected in the spleen at 4 dpi in both AB6 and AGB6 mice in the red pulp and outer marginal zone area. c 17D-204-infected AB6 mice do not display major histological changes but infected AGB6 mice and Angola71-infected animals had microsteatosis (inset) at 4 dpi. In AGB6 mice, microsteatosis only occurs transiently at 4 dpi and resolved by 11 dpi. Antibody-stained frozen liver sections (d) revealed that YFV antigen could only be detected in infected AGB6 mice at 4 dpi but not AB6 mice, parallel to titer data in Fig. 1g. Original magnification = 40x, n = 4 Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41541-017-0039-z), licensed under a CC-BY license. Not internally tested by R&D Systems.

Molecular characterization of LECs in the SCS ceiling with RNA FISH.(A-B) Expression of new cLEC/cluster 2 marker genes Ackr3 (A) and Btnl9 (B) by RNA sequencing (left panels) and RNA FISH (right panels). As GFP fluorescence is lost during tissue processing for RNA FISH, immunofluorescence staining for ANXA2 (red) and LYVE1 (green) served as markers for cLECs and fLECs, respectively. Arrows point to cLECs expressing Ackr3 and Btnl9 transcripts (white). ACKR3, atypical chemokine receptor 3; ANXA2, annexin A2; Btnl9, butyrophilin like 9; cLEC, ceiling LEC; FISH, fluorescence in situ hybridization; fLEC, floor-lining LEC; LEC, lymphatic endothelial cell; LYVE1, lymphatic vessel endothelial hyaluronan receptor 1; SCS, subcapsular sinus; UMAP, Uniform Manifold Approximation and Projection. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32251437), licensed under a CC-BY license. Not internally tested by R&D Systems.

Molecular characterization of LECs in the SCS ceiling with immunofluorescence staining.(A–C) Expression of new cLEC/cluster 2 marker genes ANXA2 (A), FABP4 (B), and CD36 (C) by RNA sequencing (left panels) and immunofluorescence staining (right panels) in Ackr4-GFP reporter mice. GFP (white) and immunofluorescence costaining for LYVE1 (green) served as markers for cLECs and fLECs, respectively. ACKR4, atypical chemokine receptor 4; ANXA2, annexin A2; CD, cluster of differentiation; cLEC, ceiling LEC; FABP4, fatty acid binding protein 4; fLEC, floor-lining LEC; GFP, green fluorescent protein; LEC, lymphatic endothelial cell; LYVE1, lymphatic vessel endothelial hyaluronan receptor 1; SCS, subcapsular sinus; UMAP, Uniform Manifold Approximation and Projection. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32251437), licensed under a CC-BY license. Not internally tested by R&D Systems.

POSTN is upregulated in (pre)-metastatic LN. Morphometric analysis of POSTN and LYVE1+ lymphatic vessels in experimental (pre)-metastatic LN (in the ear sponge assay using B16F10 cells). CTRLs correspond to mice implanted with a sponge without tumor cells. a–d–g Immunostaining of POSTN (red) and LYVE1 (green) in pre-metastatic (PM) (at 1 week in A, at 2 weeks in d) and in metastatic (M+) LNs (G). Bars = 250 µm. b–e–h Scatter graphs use scatter plots to represent POSTN and LYVE1 densities (in percentage) assessed by a computer assisted method (n ≥ 9). Results are expressed as mean ± SD, and statistical analyses were performed using a Wilcoxon–Mann–Whitney test (*p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001). c–f–i Spatial distribution analysis from tissue edge to tissue center. The blue rectangle indicates the area between 0–0.30 mm from the LN border where the cumulate normalized areas of LYVE1 and POSTN were measured and represented in the top right. Maximum distance of migration from the tissue border (Lmax) is indicated. Results are expressed as mean ± SD (Wilcoxon–Mann–Whitney test: *p < 0.05; **p < 0.01). d–i All the results represent the set of two independent experiments Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35567669), licensed under a CC-BY license. Not internally tested by R&D Systems.

AF2125) at 5ug/mL with overnight incubation at 4 degrees Celsius followed by incubation with anti-goat IgG VisUCyte HRP Polymer Antibody (Catalog # VC004) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). Specific staining was localized to endothelial cells of sinusoids. " class="big_lightbox">

Formalin-fixed paraffin-embedded tissue sections of mouse liver were probed for LYVE1 mRNA (ACD RNAScope Probe, catalog # 428451; Fast Red chromogen, ACD catalog # 322360). Adjacent tissue section was processed for immunohistochemistry using goat anti-mouse LYVE1 polyclonal antibody (R&D Systems catalog # <a class=

AF2125) at 5ug/mL with overnight incubation at 4 degrees Celsius followed by incubation with anti-goat IgG VisUCyte HRP Polymer Antibody (Catalog # VC004) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). Specific staining was localized to endothelial cells of sinusoids. " class="big_thumb" />

LECKrasG12D embryos have enlarged jugular lymph sacs & malformed lymphovenous valves. (A). Schematics of the Lyve1-Cre & KrasLSL-G12D alleles. (B). Representative images of E14.5 LECCtrl & LECKrasG12D embryos. The LECKrasG12D embryo has edema. (C). Transverse sections of E14.5 embryos stained with an anti-Lyve1 antibody (brown) & hematoxylin (purple). (D). Jugular lymph sac area was significantly greater in LECKrasG12D embryos (273391 ± 47694; n = 6 mice) than LECCtrl embryos (76949 ± 17566; n = 5 mice). (E). Coronal sections of E14.5 embryos stained with hematoxylin & eosin (H&E). The arrow points to a lymphovenous valve in a LECCtrl embryo. The arrow points to a cluster of cells in the lymphovenous valve region in a LECKrasG12D embryo. Six LECCtrl & five LECKrasG12D embryos were analyzed. (F). Coronal sections of E14.5 embryos stained with DAPI (blue) & antibodies against Lyve1 (yellow) & endomucin (red). The arrow points to a lymphovenous valve in a LECCtrl embryo. The arrow points to a cluster of Lyve1-positive cells in the lymphovenous valve region in a LECKrasG12D embryo. Five LECCtrl & three LECKrasG12D embryos were analyzed. (G). Immunostaining revealed that cell clusters in the jugular lymph sacs of LECKrasG12D embryos contained Prox1 & Lyve-1 double-positive LECs. Six LECKrasG12D embryos were analyzed. (H). Higher magnification image of cluster in panel (G). (I,J). Clusters also contained F4/80-positive cells (I) & CD45-positive cells (J). (K). Immunostaining revealed that clusters in LECKrasG12D embryos contained Runx1-positive cells. (L–O). Higher magnification images of the separate channels in panel (K). Data are presented as mean ± SEM. **p < 0.01; unpaired Student’s t-tests. Scale bar in panel C = 200 µm. Scale bars in panels E, G, I, J, & K = 100 µm. Image collected & cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/37842094), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

, ,

Applications: , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF2125U-100UG
Alexa Fluor 405	AF2125V-100UG
Alexa Fluor 488	AF2125G-100UG
Alexa Fluor 532	AF2125X-100UG
Alexa Fluor 594	AF2125T-100UG
Alexa Fluor 647	AF2125R-100UG
Alexa Fluor 700	AF2125N-100UG
Alexa Fluor 750	AF2125S-100UG
Alexa Fluor Plus 405	AF2125AFP405-100UG
Alexa Fluor Plus 488	AF2125AFP488-100UG
Alexa Fluor Plus 555	AF2125AFP555-100UG
Alexa Fluor Plus 594	AF2125AFP594-100UG
Alexa Fluor Plus 647	AF2125AFP647-100UG
Alexa Fluor Plus 680	AF2125AFP680-100UG
Allophycocyanin	AF2125A
Biotin	BAF2125
CoraFluor 1	AF2125CL1
DyLight 350	AF2125D
DyLight 405	AF2125E
DyLight 488	AF2125K
DyLight 550	AF2125L
DyLight 594	AF2125M
DyLight 650	AF2125W
DyLight 680	AF2125Y
DyLight 755	AF2125Z
FITC	AF2125F
HRP	AF2125H
Janelia Fluor 525	AF2125JF525
Janelia Fluor 549	AF2125I
Janelia Fluor 646	AF2125J
Janelia Fluor 669	AF2125JF669
PE	AF2125P
PerCP	AF2125C
mFluor Violet 450 SE	AF2125MFV450
mFluor Violet 500 SE	AF2125MFV500
mFluor Violet 610 SE	AF2125MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF2125-SP

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NB600-1008

LYVE-1 Antibody - Azide and BSA Free

(3 Reviews)

(37 Publications)

0.2 mg

NB600-1008

Western Blot: LYVE-1 Antibody [NB600-1008] - Analysis of anti-mouse LYVE-1. Sample was loaded in 15% SDS-polyacrylamide gel under reducing conditions.

LYVE-1 Antibody-Immunohistochemistry-NB600-1008-img0057.jpg

Immunohistochemistry-Paraffin: LYVE-1 Antibody [NB600-1008] - Mouse liver. Antibody at 1:800. Detection by Polymer-HRP. Image from verified customer review.

LYVE-1-Antibody-Immunohistochemistry-NB600-1008-img0052.jpg

Hypoxia, blood vessel & proliferation staining. IHC staining in the Biopsy 23 & Biopsy 3 respectively for CA-9, EF5, CD31, Ki67 & LYVE1. 15× Magnification. Image collected & cropped by CiteAb from the following publication (//www.mdpi.com/2072-6694/4/3/821), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunohistochemistry: LYVE-1 Antibody [NB600-1008] - Zeb1-regulation of alkali-induced corneal NV in mice.a Reduction of Zeb1 mRNA in the heterozygous Zeb−/+ corneas compared to the homozygous Zeb1+/+ corneas. b Significant NV score & vessel size reduction in the Zeb1−/+ corneas as compared to their Zeb1+/+ siblings. c1 A representative stereoscopic image of the Zeb1−/+ & d1 Zeb1+/+ corneas & c2–d2 their whole flat-mount corneas immunostained with the endothelium marker CD31 & the lymphatic vessel marker LYVE-1. *p ≤ 0.05; **p ≤ 0.01. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32620870), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , , ,

Applications: , , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NB600-1008SS

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AF2089

LYVE-1 Antibody [Unconjugated]

(49 Publications)

100 μg

AF2089

CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox">

LYVE‑1 was detected in immersion fixed paraffin-embedded sections of human tonsil using 15 µg/mL Goat Anti-Human LYVE‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2089) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # <a class=

CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." alt="LYVE‑1 was detected in immersion fixed paraffin-embedded sections of human tonsil using 15 µg/mL Goat Anti-Human LYVE‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2089) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

HAF019). A specific band was detected for LYVE-1 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8." class="big_lightbox">

Western blot shows lysates of human liver and spleen tissue. PVDF membrane was probed with 0.25-1 µg/mL of Goat Anti-Human LYVE-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2089) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=

HAF019). A specific band was detected for LYVE-1 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8." class="big_thumb" />

Overview of Multi-dimensional Microscopic Molecular Profiling (MMMP).The overall MMMP approach is depicted using an example tissue section from normal human duodenum (sample #1.9.7). (a) Slides were subjected to repeated cycles of staining and imaging with fluorescent primary antibodies and DAPI. At the end of each cycle, fluorescent signal was removed by a chemical bleaching process, and slides were again imaged, before proceeding to the next round of this iterative procedure. After the final antibody stain (#15 Sma), slides were analyzed with a series of histochemical stains. (b) A set of tiling images spanning each tissue section was initially generated by the microscope system. The tiling images were then computationally ‘stitched’ together to produce a single image per staining cycle for each sample. (c) Image registration was performed to align images from the same tissue section across cycles. Mean intensities of the DAPI signal from all immuno-fluorescence images are shown from before (Unregistered) and after (Registered) the image registration procedure was completed. (d) Following registration, signal intensities from the relevant channels for each image (columns) in the MMMP series were extracted for each pixel (rows) within the tissue section and compiled into a large data matrix of in situ molecular profiles. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0128975), licensed under a CC-BY license. Not internally tested by R&D Systems.

<b>Biological Strategies Validation. </b>TGF-beta 1, -beta 2 and -beta 3 reduce lymphatic marker expression in LECs.Primary human LECs were treated with 10, 20 or 30 ng/ml TGF-beta 1, -beta 2 and -beta 3 for 72 hours (a) or 100 hours (b). Untreated cells served as a control. Lysates were prepared and analysed by Western blot using antibodies specific for Lyve-1, Prox-1, VEGFR-3 or vimentin. Vinculin served as loading control. The experiment was performed twice with equivalent results. For densitometry evaluation, protein bands were analysed using the software ImageJ. Bands for the Prox-1, Lyve-1, vimentin and VEGFR-3 proteins were normalized to the corresponding loading control and are displayed as the expression level relative to the untreated control samples. Image collected and cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0162221), licensed under a CC-BY license. Not internally tested by R&D Systems.

Peritumoral LECs upregulate MHC-II, PD-L1, and various co-inhibitory molecules compared to naïve dermal LECs. (A) Schematic of experimental design analyzing naïve dermal LECs in the ear and peritumoral LECs of the same mice. (B) Quantification of MHC-II and PD-L1 positivity in naïve and peritumoral LECs (CD45- podoplanin+ CD31+) at day 7, 14, and 21 post B16F10 tumor inoculation in WT (C57/BL6) mice. (C) Quantification of MHC-II and PD-L1 positivity in naïve and peritumoral LECs at day 14 post E0771 tumor inoculation in WT mice. (D) Quantification of MHC-II and PD-L1 positivity in naïve and peritumoral LECs in MMTV-PyMT mice with spontaneous mammary tumors. (E) Quantification of PD-L2, HVEM, and CD48 positivity in naïve and peritumoral LECs at day 21 post B16F10 tumor inoculation in WT mice. (F) Representative immunofluorescence image stained for LYVE-1 (white) and MHC-II (green) in peritumoral and skin margin lymphatics in WT mice at day 14 post B16F10 inoculation. DAPI nuclear staining in blue. Scale bars, 100 μm (left), 20μm (top right and bottom right). Statistical significance assessed using paired student’s t test on data from at least 3 biological replicates; error represented as SD. *, p < 0.05; **, p < 0.01; ***, p < 0.001. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36362253), licensed under a CC-BY license. Not internally tested by R&D Systems.

2089-LY) was serially diluted and captured by Mouse Anti-Human LYVE‑1 Monoclonal Antibody (Catalog # MAB20891) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human LYVE‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2089) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox">

Recombinant Human LYVE‑1 (Catalog # <a class=

2089-LY) was serially diluted and captured by Mouse Anti-Human LYVE‑1 Monoclonal Antibody (Catalog # MAB20891) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human LYVE‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2089) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF2089U-100UG
Alexa Fluor 405	AF2089V-100UG
Alexa Fluor 488	AF2089G-100UG
Alexa Fluor 532	AF2089X-100UG
Alexa Fluor 594	AF2089T-100UG
Alexa Fluor 647	AF2089R-100UG
Alexa Fluor 700	AF2089N-100UG
Alexa Fluor 750	AF2089S-100UG
Alexa Fluor Plus 405	AF2089AFP405-100UG
Alexa Fluor Plus 488	AF2089AFP488-100UG
Alexa Fluor Plus 555	AF2089AFP555-100UG
Alexa Fluor Plus 594	AF2089AFP594-100UG
Alexa Fluor Plus 647	AF2089AFP647-100UG
Alexa Fluor Plus 680	AF2089AFP680-100UG
Allophycocyanin	AF2089A
Biotin	BAF2089
CoraFluor 1	AF2089CL1
DyLight 350	AF2089D
DyLight 405	AF2089E
DyLight 488	AF2089K
DyLight 550	AF2089L
DyLight 594	AF2089M
DyLight 650	AF2089W
DyLight 680	AF2089Y
DyLight 755	AF2089Z
FITC	AF2089F
HRP	AF2089H
Janelia Fluor 525	AF2089JF525
Janelia Fluor 549	AF2089I
Janelia Fluor 646	AF2089J
Janelia Fluor 669	AF2089JF669
PE	AF2089P
PerCP	AF2089C
mFluor Violet 450 SE	AF2089MFV450
mFluor Violet 500 SE	AF2089MFV500
mFluor Violet 610 SE	AF2089MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF2089-SP

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MAB2125

LYVE-1 Antibody (223322) [Unconjugated]

(41 Publications)

100 μg

MAB2125

HAF005). A specific band was detected for LYVE-1 at approximately 65 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

Western blot shows lysate of bEnd.3 mouse endothelioma cell line. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse LYVE-1 Monoclonal Antibody (Catalog # MAB2125) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (<a class=

HAF005). A specific band was detected for LYVE-1 at approximately 65 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysate of bEnd.3 mouse endothelioma cell line. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse LYVE-1 Monoclonal Antibody (Catalog # MAB2125) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (HAF005). A specific band was detected for LYVE-1 at approximately 65 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

MAB006, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (F0105B)." class="big_lightbox">

bEnd.3 mouse endothelioma cell line was stained with Rat Anti-Mouse LYVE-1 Monoclonal Antibody (Catalog # MAB2125, filled histogram) or isotype control antibody (<a class=

MAB006, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (F0105B)." class="big_thumb" />

NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox">

LYVE-1 was detected in immersion fixed NS0 mouse myeloma cell line transfected with mouse LYVE-1 (positive control) and wild type NS0 mouse myeloma cell line (negative control) using Rat Anti-Mouse LYVE-1 Monoclonal Antibody (Catalog # MAB2125) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; <a class=

NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

VC005). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to endothelial cells in lymphatic vessels. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox">

LYVE-1 was detected in immersion fixed frozen sections of mouse intestine tissue using Rat Anti-Mouse LYVE-1 Monoclonal Antibody (Catalog # MAB2125) at 15 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rat IgG VisUCyte™ HRP Polymer Antibody (<a class=

VC005). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to endothelial cells in lymphatic vessels. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

, , , ,

Applications: , , , ,

Host:

Rat Monoclonal

Host: Rat Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB2125U-100UG
Alexa Fluor 405	FAB2125V-100UG
Alexa Fluor 488	FAB2125G
Alexa Fluor 532	FAB2125X
Alexa Fluor 594	FAB2125T-100UG
Alexa Fluor 647	FAB2125R-100UG
Alexa Fluor 700	FAB2125N-100UG
Alexa Fluor 750	FAB2125S-100UG
Alexa Fluor Plus 405	FAB2125AFP405-100UG
Alexa Fluor Plus 488	FAB2125AFP488-100UG
Alexa Fluor Plus 555	FAB2125AFP555-100UG
Alexa Fluor Plus 594	FAB2125AFP594-100UG
Alexa Fluor Plus 647	FAB2125AFP647-100UG
Alexa Fluor Plus 680	FAB2125AFP680-100UG
Allophycocyanin	FAB2125A
Allophycocyanin/Cy7	FAB2125APCCY7
Biotin	FAB2125B
CoraFluor 1	FAB2125CL1
DyLight 350	FAB2125D
DyLight 405	FAB2125E
DyLight 488	FAB2125K
DyLight 550	FAB2125L
DyLight 594	FAB2125M
DyLight 650	FAB2125W
DyLight 680	FAB2125Y
DyLight 755	FAB2125Z
FITC	FAB2125F
HRP	FAB2125H
Janelia Fluor 525	FAB2125JF525
Janelia Fluor 549	FAB2125I
Janelia Fluor 646	FAB2125J
Janelia Fluor 669	FAB2125JF669
PE/Atto594	FAB2125PEATT594
PE/Cy5.5	FAB2125PECY55
PE/Cy7	FAB2125PECY7
PerCP	FAB2125C
Phycoerythrin	FAB2125P
mFluor Violet 450 SE	FAB2125MFV450
mFluor Violet 500 SE	FAB2125MFV500
mFluor Violet 610 SE	FAB2125MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	FAB2125P
Carrier Free	FAB2125A
Carrier Free	FAB2125G
Carrier Free	MAB2125-SP
Carrier Free	MAB2125-500
Carrier Free	FAB2125V-100UG
Carrier Free	FAB2125U-100UG
Carrier Free	FAB2125T-100UG
Carrier Free	FAB2125S-100UG
Carrier Free	FAB2125R-100UG
Carrier Free	FAB2125N-100UG

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NB100-725

LYVE-1 Antibody - BSA Free

(2 Reviews)

(12 Publications)

0.1 ml

NB100-725

Immunohistochemistry: LYVE-1 Antibody [NB100-725] - Detection of LYVE1 in endothelial cells of human lung blood vessels using NB100-725. Note presence of RBCs within vessel lumen.

Flow Cytometry: LYVE-1 Antibody [NB100-725] - Analysis using the DyLight 488 conjugate of NB100-725. Unlabeled control with labeled samples of cells dissociated mechanically and enzymatically from human skin. Image provided via product review by Patricia Redondo.

Western Blot: LYVE-1 Antibody [NB100-725] - Analysis of extracts from A549 cells using LYVE1 antibody (NB100-725, 1:100). Image from verified customer review.

Flow Cytometry: LYVE-1 Antibody [NB100-725] - LYVE1 antibody was tested at 1:400 in A549 cells using an Alexa Fluor 488 secondary (shown in green) alongside unstained cells (shown in red).

Flow Cytometry: LYVE-1 Antibody [NB100-725] - An intracellular stain was performed on A549 cells with LYVE-1 Antibody NB100-725 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).

Immunohistochemistry: LYVE-1 Antibody [NB100-725] - Detection of LYVE1 in endothelial cells of human bladder vasculature using NB100-725.

Immunohistochemistry: LYVE-1 Antibody [NB100-725] - Detection of LYVE1 in endothelial cells of human lung blood vessels using NB100-725.

Flow Cytometry: LYVE-1 Antibody [NB100-725] - Analysis using the Alexa Fluor (R) 488 conjugate of NB100-725. Staining of LYVE1 in human dissociated skin lyve1+ using AF488 conjugated anti-LYVE1 antibody. Image from verified customer review.

Flow Cytometry: LYVE-1 Antibody [NB100-725] - An intracellular stain was performed on A549 cells with LYVE-1 Antibody NB100-725G (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Dylight 488.

Flow Cytometry: LYVE-1 Antibody [NB100-725] - An intracellular stain was performed on K562 cells with LYVE-1 Antibody NB100-725C (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 650.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB100-725AF350
Alexa Fluor 405	NB100-725AF405
Alexa Fluor 488	NB100-725AF488
Alexa Fluor 532	NB100-725AF532
Alexa Fluor 594	NB100-725AF594
Alexa Fluor 647	NB100-725AF647
Alexa Fluor 700	NB100-725AF700
Alexa Fluor 750	NB100-725AF750
Alexa Fluor Plus 405	NB100-725AFP405
Alexa Fluor Plus 488	NB100-725AFP488
Alexa Fluor Plus 555	NB100-725AFP555
Alexa Fluor Plus 594	NB100-725AFP594
Alexa Fluor Plus 647	NB100-725AFP647
Alexa Fluor Plus 680	NB100-725AFP680
Allophycocyanin	NB100-725APC
Allophycocyanin/Cy7	NB100-725APCCY7
Biotin	NB100-725B
CoraFluor 1	NB100-725CL1
DyLight 350	NB100-725UV
DyLight 405	NB100-725V
DyLight 488	NB100-725G
DyLight 550	NB100-725R
DyLight 594	NB100-725DL594
DyLight 650	NB100-725C
DyLight 680	NB100-725FR
DyLight 755	NB100-725IR
FITC	NB100-725F
HRP	NB100-725H
Janelia Fluor 525	NB100-725JF525
Janelia Fluor 549	NB100-725JF549
Janelia Fluor 646	NB100-725JF646
Janelia Fluor 669	NB100-725JF669
PE	NB100-725PE
PE/Atto594	NB100-725PEATT594
PE/Cy5.5	NB100-725PECY55
PE/Cy7	NB100-725PECY7
PerCP	NB100-725PCP
mFluor Violet 450 SE	NB100-725MFV450
mFluor Violet 500 SE	NB100-725MFV500
mFluor Violet 610 SE	NB100-725MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB100-725SS

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NB100-939

LYVE-1 Antibody Pack

1 Pack

NB100-939

Western Blot: LYVE-1 Antibody Pack [NB100-939] - Detection of LYVE-1 in mouse spleen lysate using NB 100-726. ECL detection overnight.

Flow Cytometry: LYVE-1 Antibody Pack [NB100-939] - Analysis using the DyLight 488 conjugate of NB100-725. Unlabeled control with labeled samples of cells dissociated mechanically and enzymatically from human skin. Image provided via product review by Patricia Redondo.

Immunohistochemistry: LYVE-1 Antibody Pack [NB100-939] - Detection of LYVE-1 in endothelial cells of human lung blood vessels using NB100-725.

Immunohistochemistry: LYVE-1 Antibody Pack [NB100-939] - Detection of LYVE-1 in endothelial cells of human bladder vasculature using NB100-725.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , ,

Applications: , , ,

Host:

Rabbit

Host: Rabbit

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NB600-1005

LYVE-1 Antibody - Azide and BSA Free

(1 Review)

0.2 mg

NB600-1005

Western Blot: LYVE-1 Antibody [NB600-1005] - Analysis of recombinant human sLYVE-1 and mouse sLYVE-1 using an anti-human LYVE-1 polyclonal antibody directed against the extracellular domain of human LYVE-1. There is more or less no cross reactivity withmouse LYVE-1.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NB600-1005-0.025mg

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NB100-726

LYVE-1 Antibody - BSA Free

0.1 ml

NB100-726

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

Applications:

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB100-726AF350
Alexa Fluor 405	NB100-726AF405
Alexa Fluor 488	NB100-726AF488
Alexa Fluor 532	NB100-726AF532
Alexa Fluor 594	NB100-726AF594
Alexa Fluor 647	NB100-726AF647
Alexa Fluor 700	NB100-726AF700
Alexa Fluor 750	NB100-726AF750
Alexa Fluor Plus 405	NB100-726AFP405
Alexa Fluor Plus 488	NB100-726AFP488
Alexa Fluor Plus 555	NB100-726AFP555
Alexa Fluor Plus 594	NB100-726AFP594
Alexa Fluor Plus 647	NB100-726AFP647
Alexa Fluor Plus 680	NB100-726AFP680
Allophycocyanin	NB100-726APC
Biotin	NB100-726B
CoraFluor 1	NB100-726CL1
DyLight 350	NB100-726UV
DyLight 405	NB100-726V
DyLight 488	NB100-726G
DyLight 550	NB100-726R
DyLight 594	NB100-726DL594
DyLight 650	NB100-726C
DyLight 680	NB100-726FR
DyLight 755	NB100-726IR
FITC	NB100-726F
HRP	NB100-726H
Janelia Fluor 525	NB100-726JF525
Janelia Fluor 549	NB100-726JF549
Janelia Fluor 646	NB100-726JF646
Janelia Fluor 669	NB100-726JF669
PE	NB100-726PE
PerCP	NB100-726PCP
mFluor Violet 450 SE	NB100-726MFV450
mFluor Violet 500 SE	NB100-726MFV500
mFluor Violet 610 SE	NB100-726MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB100-726SS

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DY2089

Human LYVE-1 DuoSet ELISA, 15 Plate

(5 Publications)

1 Kit (for 15 Plates)

DY2089

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

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AF7939

LYVE-1 Antibody

100 μg

AF7939

HAF016). A specific band was detected for LYVE-1 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

Western blot shows lysates of rat lymph node tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Rat LYVE-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7939) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # <a class=

HAF016). A specific band was detected for LYVE-1 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of rat lymph node tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Rat LYVE-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7939) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for LYVE-1 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to glomeruli. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_lightbox">

LYVE-1 was detected in perfusion fixed frozen sections of rat kidney using Sheep Anti-Rat LYVE-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7939) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # <a class=

CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to glomeruli. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_thumb" />

Reactivity:

Rt

Species Glossary

Reactivity: Rt

Applications:

,

Applications: ,

Host:

Sheep Polyclonal

Host: Sheep Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF7939U-100UG
Alexa Fluor 405	AF7939V-100UG
Alexa Fluor 488	AF7939G-100UG
Alexa Fluor 532	AF7939X-100UG
Alexa Fluor 594	AF7939T-100UG
Alexa Fluor 647	AF7939R-100UG
Alexa Fluor 700	AF7939N-100UG
Alexa Fluor 750	AF7939S-100UG
Alexa Fluor Plus 405	AF7939AFP405-100UG
Alexa Fluor Plus 488	AF7939AFP488-100UG
Alexa Fluor Plus 555	AF7939AFP555-100UG
Alexa Fluor Plus 594	AF7939AFP594-100UG
Alexa Fluor Plus 647	AF7939AFP647-100UG
Alexa Fluor Plus 680	AF7939AFP680-100UG
Allophycocyanin	AF7939A
Biotin	AF7939B
CoraFluor 1	AF7939CL1
DyLight 350	AF7939D
DyLight 405	AF7939E
DyLight 488	AF7939K
DyLight 550	AF7939L
DyLight 594	AF7939M
DyLight 650	AF7939W
DyLight 680	AF7939Y
DyLight 755	AF7939Z
FITC	AF7939F
HRP	AF7939H
Janelia Fluor 525	AF7939JF525
Janelia Fluor 549	AF7939I
Janelia Fluor 646	AF7939J
Janelia Fluor 669	AF7939JF669
PE	AF7939P
PerCP	AF7939C
mFluor Violet 450 SE	AF7939MFV450
mFluor Violet 500 SE	AF7939MFV500
mFluor Violet 610 SE	AF7939MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF7939-SP

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NBP3-26159

LYVE-1 Antibody (11C11) - BSA Free

100 ul

NBP3-26159

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP3-26159-50ul

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MAB20891

LYVE-1 Antibody (264725)

(1 Publication)

500 μg

MAB20891

2089-LY) was serially diluted and captured by Mouse Anti-Human LYVE‑1 Monoclonal Antibody (Catalog # MAB20891) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human LYVE‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2089) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox">

2089-LY) was serially diluted and captured by Mouse Anti-Human LYVE‑1 Monoclonal Antibody (Catalog # MAB20891) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human LYVE‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2089) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" alt="Recombinant Human LYVE‑1 (Catalog # 2089-LY) was serially diluted and captured by Mouse Anti-Human LYVE‑1 Monoclonal Antibody (Catalog # MAB20891) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human LYVE‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2089) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB20891U-100UG
Alexa Fluor 405	FAB20891V-100UG
Alexa Fluor 488	FAB20891G-100UG
Alexa Fluor 532	FAB20891X-100UG
Alexa Fluor 594	FAB20891T-100UG
Alexa Fluor 647	FAB20891R-100UG
Alexa Fluor 700	FAB20891N-100UG
Alexa Fluor 750	FAB20891S-100UG
Alexa Fluor Plus 405	FAB20891AFP405-100UG
Alexa Fluor Plus 488	FAB20891AFP488-100UG
Alexa Fluor Plus 555	FAB20891AFP555-100UG
Alexa Fluor Plus 594	FAB20891AFP594-100UG
Alexa Fluor Plus 647	FAB20891AFP647-100UG
Alexa Fluor Plus 680	FAB20891AFP680-100UG
Allophycocyanin	FAB20891A
Biotin	FAB20891B
CoraFluor 1	FAB20891CL1
DyLight 350	FAB20891D
DyLight 405	FAB20891E
DyLight 488	FAB20891K
DyLight 550	FAB20891L
DyLight 594	FAB20891M
DyLight 650	FAB20891W
DyLight 680	FAB20891Y
DyLight 755	FAB20891Z
FITC	FAB20891F
HRP	FAB20891H
Janelia Fluor 525	FAB20891JF525
Janelia Fluor 549	FAB20891I
Janelia Fluor 646	FAB20891J
Janelia Fluor 669	FAB20891JF669
PE	FAB20891P
PerCP	FAB20891C
mFluor Violet 450 SE	FAB20891MFV450
mFluor Violet 500 SE	FAB20891MFV500
mFluor Violet 610 SE	FAB20891MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB20891-SP

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Reviews

Host

Available Conjugates

Format

Modification

Clonality

Clone

Images Type

Isotype

Preservative

Protein Or Peptide

Research Areas

Has Sample Size

Promotional Item

Species Glossary

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LYVE-1 Antibody

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