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210-TA, filled histogram) or rested (open histogram) and stained with Mouse anti-human E-selectin/CD62E (BBA16) followed by Goat anti-Mouse APC-conjugated secondary antibody (F0101B). Gates were set based on isotype control (MAB002, data not shown). Staining was performed using our Staining Membrane-associated Proteins protocol. " class="big_lightbox" target="_blank">
ADP1) was serially diluted and captured by Mouse Anti-Human E‑Selectin/CD62E Monoclonal Antibody (Catalog # BBA16) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human E‑Selectin/CD62E Biotinylated Monoclonal Antibody (Catalog # [[catalogNumber:) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # [[catalogNumber:DY998)" class="big_lightbox" target="_blank">
210-TA, filled histogram) or rested (open histogram) and stained with Mouse anti-human E-selectin/CD62E (BBA26) followed by Goat anti-Mouse APC-conjugated secondary antibody (F0101B). Gates were set based on isotype control (MAB002, data not shown). Staining was performed using our Staining Membrane-associated Proteins protocol. " class="big_lightbox" target="_blank">
210-TA) using Mouse Anti-Human E-Selectin/CD62E Monoclonal Antibody (Catalog # BBA26) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox" target="_blank">
CD62E (also known as E-selectin, ELAM-1, and LECAM-1) is a 115 kD type I membrane protein and a member of the selectin family. CD62E is highly expressed on activated endothelial cells (IL-2, TNF- alpha, other cytokines can increase expression) and can also be expressed on endothelial cells in the skin, bone marrow and placenta. CD62E is involved in tethering and leucocyte rolling on activated endothelium at inflammatory sites and may also play a role in tumor metastasis and angiogenesis. CD62E binds to both Siayl Lewis X (CD15s) and PSGL-1 (CD162). The HCD62E antibody has been shown to recognize human CD62E and to be useful for flow cytometry.
![Flow Cytometry E-Selectin/CD62E Antibody (BBIG-E4) [Unconjugated]](http://images.novusbio.com/images/antibody/bba16_human-e-selectin-cd62e-mab-clone-bbig-e4-flow-cytometry-25102021144214.jpg)
![Immunohistochemistry E-Selectin/CD62E Antibody [Unconjugated]](http://images.novusbio.com/images/antibody/E-Selectin_BBA18_Immunohistochemistry_6939.jpg)
![Flow Cytometry E-Selectin/CD62E Antibody (BBIG-E1) [Unconjugated]](http://images.novusbio.com/images/antibody/bba26_human-e-selectin-cd62e-mab-cl-bbig-e1-flow-cytometry-2510202115158.jpg)
![Sandwich ELISA E-Selectin/CD62E Antibody Pair [HRP]](http://images.novusbio.com/fullsize/E-Selectin-CD62E-Antibody-Pair-[HRP]-Sandwich-ELISA-NBP2-79577-img0001.jpg)
![Sandwich ELISA E-Selectin/CD62E Antibody Pair [HRP]](http://images.novusbio.com/fullsize/E-Selectin-CD62E-Antibody-Pair-[HRP]-Sandwich-ELISA-NBP2-79633-img0001.jpg)
![N/A E-Selectin/CD62E [HRP]](http://images.novusbio.com/fullsize/elisa/DATA_ESelectin_DSLE00_ELISA_828.jpg)
![N/A E-Selectin/CD62E [HRP]](http://images.novusbio.com/fullsize/elisa/DATA_ESelectin_MES00_ELISA_971.jpg)
![N/A E-Selectin/CD62E [Biotin]](http://images.novusbio.com/fullsize/elisa/DATA_ESelectin_DY724_ELISA_2195.jpg)
![Immunocytochemistry E-Selectin/CD62E Antibody (BBIG-E4) [Unconjugated]](http://images.novusbio.com/images/antibody/E-Selectin_BBA16_Immunocytochemistry_9821.jpg)
![Immunohistochemistry E-Selectin/CD62E Antibody (BBIG-E4) [Unconjugated]](http://images.novusbio.com/images/antibody/E-Selectin_BBA16_Immunohistochemistry_6938.jpg)
![Western Blot E-Selectin/CD62E Antibody (BBIG-E4) [Unconjugated]](http://images.novusbio.com/fullsize/bba16_human-e-selectin-cd62e-mab-clone-bbig-e4-western-blot-1212202581524.jpg)
![Western Blot E-Selectin/CD62E Antibody (BBIG-E4) [Unconjugated]](http://images.novusbio.com/fullsize/bba16_human-e-selectin-cd62e-mab-clone-bbig-e4-western-blot-121220258175721.jpg)
![ELISA E-Selectin/CD62E Antibody (BBIG-E4) [Unconjugated]](http://images.novusbio.com/images/bba16_human-e-selectin-cd62e-mab-clone-bbig-e4-elisa-639201780597376993.jpg)
![Immunohistochemistry E-Selectin/CD62E Antibody [Unconjugated]](http://images.novusbio.com/fullsize/antibody/ESelectin_BBA18_Immunohistochemistry_22158.jpg)
![Immunocytochemistry E-Selectin/CD62E Antibody (BBIG-E1) [Unconjugated]](http://images.novusbio.com/images/antibody/ESelectin_BBA26_Immunocytochemistry__Immunofluorescence_18918.jpg)
![N/A E-Selectin/CD62E [HRP]](http://images.novusbio.com/fullsize/elisa/E-Selectin_DSLE00_ELISA_183.jpg)
![N/A E-Selectin/CD62E [HRP]](http://images.novusbio.com/fullsize/elisa/E-Selectin_MES00_ELISA_264.jpg)
