WB, ChIP, ELISA, EM, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IM, IP, ISH, IHC-FrFl, IHC-WhMtHost:
WB, ELISA, EM, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IPHost:
WB, ELISA, EM, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, PLA, IHC-FrFlHost:
Green fluorescence protein (GFP) is a 27 kDa protein, originally derived from the jellyfish Aequorea victoria. Researchers have long used GFP for its fluorescent property, as it emits green light (emission peak of 509 nm) when excited by blue light (excitation peak of 395 nm). GFP's intrinsic fluorescence can be visualized in living cells, is stable under fixation conditions and is suitable for a variety of applications. This protein has therefore been widely used as a reporter for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. Other applications of GFP include assessment of protein-protein interactions through the yeast two hybrid system and measurement of distance between proteins through fluorescence energy transfer (FRET) protocols. GFP technnology has considerably contributed to a greater understanding of cellular physiology.
- green fluorescent protein (gfp)
- Green Fluorescent Protein
- Enhanced Green Fluorescent Protein