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314-BP) induces alkaline phosphatase production in the the C2C12 mouse myoblast cell line in a dose-dependent manner (orange line). Alkaline phosphatase production elicited by Recombinant Human BMP‑4 (500 ng/mL) is neutralized (green line) by increasing concentrations of Human BMP‑4 Monoclonal Antibody (Catalog # MAB757). The ND50 is typically 1-3 µg/mL in the presence of heparin (2 µg/mL)." class="big_lightbox" target="_blank">
314-BP) induces alkaline phosphatase production in the the ATDC5 mouse chondrogenic cell line in a dose-dependent manner (orange line). Alkaline phosphatase production elicited by Recombinant Human BMP-4 (15 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human BMP-4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF757). The ND50 is typically 0.5-2.0 µg/mL in the presence of heparin (2 µg/mL)." class="big_lightbox" target="_blank">
AFL314E) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 8-9 kDa and 20-22 kDa, respectively." class="big_lightbox" target="_blank">
314E-GMP), Animal-Free (Catalog # AFL314E) and RUO (314-BPE) grades of Recombinant Human BMP-4 as measured by its ability to induce BMP responsive SEAP reporter activity in HEK293 human embryonic kidney cells (orange, green, red, respectively)." class="big_lightbox" target="_blank">
Bone morphogenetic proteins were originally identified by an ability of demineralized bone extract to induce endochondral osteogenesis in vivo in an extraskeletal site. Through molecular cloning, 7 BMP cDNAs, designated BMP1 through BMP7, have been recovered. BMP2 through BMP7, are members of the transforming growth factor-beta superfamily of regulatory molecules. Transcript variants utilizing alternative promoters and alternative non-coding first exons have been described in the literature.
![Neutralization BMP-4 Antibody (66119) [Unconjugated]](http://images.novusbio.com/images/antibody/BMP-4_MAB757_Block_Neutralize_9272.jpg)
![Neutralization BMP-4 Antibody [Unconjugated]](http://images.novusbio.com/images/antibody/BMP-4_AF757_Block_Neutralize_8959.jpg)
![N/A BMP-4 [HRP]](http://images.novusbio.com/fullsize/elisa/DATA_BMP4_DBP400_ELISA_594.jpg)
![N/A BMP-4 [Biotin]](http://images.novusbio.com/fullsize/elisa/DATA_BMP4_DY314_ELISA_1920.jpg)
![Bioactivity BMP-4 [Unconjugated]](http://images.novusbio.com/fullsize/314-bp_recombinant-human-bmp-4-protein-1882021123744.jpg)
![Bioactivity BMP-4 [Unconjugated]](http://images.novusbio.com/fullsize/protein/afl314e_recombinant-human-bmp-4-animal-free-protein-bioactivity-12620209179.jpg)
![Western Blot BMP-4 Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af757_human-bmp-4-affinity-purified-polyclonal-ab-812025557251.jpg)
![N/A BMP-4 [HRP]](http://images.novusbio.com/fullsize/elisa/BMP-4_DBP400_ELISA_40.jpg)
![SDS-Page BMP-4 [Unconjugated]](http://images.novusbio.com/fullsize/protein/BMP-4_314-BP_100.jpg)
![SDS-Page BMP-4 [Unconjugated]](http://images.novusbio.com/images/protein/afl314e_recombinant-human-bmp-4-animal-free-protein-sds-page-962020134757.jpg)
![Bioactivity BMP-4 [Unconjugated]](http://images.novusbio.com/images/afl314e_recombinant-human-bmp-4-animal-free-protein-bioactivity-1532022104128.jpg)
