Immunoprecipitation (IP) is a common research technique used to precipitate a specific protein (or protein complex) of interest out of a heterogeneous lysate. The concept of precipitating proteins out of solution is often called a "pull-down". There are a variety of methods to achieve immunoprecipitation, the most common of which uses antibodies bound to magnetic or agarose beads. This process can be used to isolate and concentrate a particular protein or complex from a sample containing many thousands of different proteins, and is often used to study protein-protein interactions through Co-IP experiments.
Novus offers a range of support options for IP when you buy a product to help you with your IP experiment including:
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-95251-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-05030-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-12899-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-47607-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-12918-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-12919-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-50416-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB110-96875-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-12884-25ug | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| FITC | NBP1-71697-500ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-12883-25ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB120-10535-0.025ml | |||
![Western Blot: Pyruvate Dehydrogenase E1-alpha subunit Antibody [NBP2-95251] - Analysis of extracts of various cell lines, using PDHA1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/Pyruvate-Dehydrogenase-E1-alpha-subunit-Antibody-Western-Blot-NBP2-95251-img0007.jpg "Western Blot: Pyruvate Dehydrogenase E1-alpha subunit Antibody [NBP2-95251] - Analysis of extracts of various cell lines, using PDHA1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 180s.")
![Immunohistochemistry-Paraffin: Pyruvate Dehydrogenase E1-alpha subunit Antibody [NBP2-95251] - Human thyroid cancer using PDHA1 Rabbit pAb at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/Pyruvate-Dehydrogenase-E1-alpha-subunit-Antibody-Immunohistochemistry-Paraffin-NBP2-95251-img0006.jpg)
![Immunohistochemistry-Paraffin: Pyruvate Dehydrogenase E1-alpha subunit Antibody [NBP2-95251] - Rat kidney using PDHA1 Rabbit pAb at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/Pyruvate-Dehydrogenase-E1-alpha-subunit-Antibody-Immunohistochemistry-Paraffin-NBP2-95251-img0005.jpg)
![Immunocytochemistry/ Immunofluorescence: Pyruvate Dehydrogenase E1-alpha subunit Antibody - Azide and BSA Free [NBP2-95251] - Immunofluorescence analysis of NIH/3T3 cells using Pyruvate Dehydrogenase E1-alpha subunit Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-95251_rabbit-polyclonal-pyruvate-dehydrogenase-e1-alpha-subunit-antibody-5220251640291.jpg)
![Western Blot: KIF3A Antibody [NBP3-05030] - Analysis of extracts of various cell lines, using KIF3A antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit](http://images.novusbio.com/fullsize/KIF3A-Antibody-Western-Blot-NBP3-05030-img0003.jpg "Western Blot: KIF3A Antibody [NBP3-05030] - Analysis of extracts of various cell lines, using KIF3A antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit")
![Immunocytochemistry/Immunofluorescence: KIF3A Antibody [NBP3-05030] - Analysis of MCF7 cells using KIF3A antibody . Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/KIF3A-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-05030-img0001.jpg)
![Immunoprecipitation: KIF3A Antibody [NBP3-05030] - Analysis of 200ug extracts of MCF-7 cells, using KIF3A antibody . Western blot was performed from the immunoprecipitate using this antibody.](http://images.novusbio.com/fullsize/KIF3A-Antibody-Immunoprecipitation-NBP3-05030-img0002.jpg)
![Immunohistochemistry-Paraffin: KIF3A Antibody [NBP3-05030] - Rat testis using KIF3A Rabbit pAb at dilution of 1:500 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/antibody/nbp3-05030_rabbit-polyclonal-kif3a-antibody-immunohistochemistry-paraffin-146202493146..jpg)
![Western Blot: Kv7.4 Antibody (S43/6) [NBP2-12899] - Western Blot analysis of Rat tissue lysate showing detection of Kv7.4 protein using Mouse Anti-Kv7.4 Monoclonal Antibody, Clone N43/6 (NBP2-12899). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Kv7.4 Monoclonal Antibody (NBP2-12899) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.](http://images.novusbio.com/fullsize/Kv7-4-Antibody-S43-6-Western-Blot-NBP2-12899-img0008.jpg "Western Blot: Kv7.4 Antibody (S43/6) [NBP2-12899] - Western Blot analysis of Rat tissue lysate showing detection of Kv7.4 protein using Mouse Anti-Kv7.4 Monoclonal Antibody, Clone N43/6 (NBP2-12899). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Kv7.4 Monoclonal Antibody (NBP2-12899) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.")
![Immunocytochemistry/Immunofluorescence: Kv7.4 Antibody (S43/6) [NBP2-12899] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Kv7.4 Monoclonal Antibody, Clone N43/6 (NBP2-12899). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-Kv7.4 Monoclonal Antibody (NBP2-12899) at 1:100 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Kv7.4 Antibody (D) Composite.](http://images.novusbio.com/fullsize/Kv7-4-Antibody-S43-6-Immunocytochemistry-Immunofluorescence-NBP2-12899-img0010.jpg)
![Immunohistochemistry: Kv7.4 Antibody (S43/6) [NBP2-12899] - Immunohistochemistry analysis using Mouse Anti-Kv7.4 Monoclonal Antibody, Clone N43/6 (NBP2-12899). Tissue: hippocampus. Species: Human. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Kv7.4 Monoclonal Antibody (NBP2-12899) at 1:1000 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT.](http://images.novusbio.com/fullsize/Kv7-4-Antibody-S43-6-Immunohistochemistry-NBP2-12899-img0009.jpg)
![Immunohistochemistry-Frozen: Kv7.4 Antibody (S43/6) [NBP2-12899] - Tissue: frozen brain section. Species: mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-KCNQ4 Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 uL for 5 minutes at RT.](http://images.novusbio.com/fullsize/Kv7-4-Antibody-S43-6-Immunohistochemistry-Frozen-NBP2-12899-img0006.jpg)
![Western Blot: CACNB2 Antibody (N8b/1) [NBP1-47607] - Western Blot analysis of Human Cell line lysates showing detection of Cav Beta2 Calcium Channel protein using Mouse Anti-Cav Beta2 Calcium Channel Monoclonal Antibody, Clone S8b-1 (NBP1-47607). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Cav Beta2 Calcium Channel Monoclonal Antibody (NBP1-47607) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.](http://images.novusbio.com/fullsize/CACNB2-Antibody-N8b-1-Western-Blot-NBP1-47607-img0006.jpg "Western Blot: CACNB2 Antibody (N8b/1) [NBP1-47607] - Western Blot analysis of Human Cell line lysates showing detection of Cav Beta2 Calcium Channel protein using Mouse Anti-Cav Beta2 Calcium Channel Monoclonal Antibody, Clone S8b-1 (NBP1-47607). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Cav Beta2 Calcium Channel Monoclonal Antibody (NBP1-47607) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.")
![Immunocytochemistry/Immunofluorescence: CACNB2 Antibody (N8b/1) [NBP1-47607] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Cav beta 2 Monoclonal Antibody, Clone S8B-1 (NBP1-47607). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-Cav beta 2 Monoclonal Antibody (NBP1-47607) at 1:50 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Cav beta 2 Antibody (D) Composite.](http://images.novusbio.com/fullsize/CACNB2-Antibody-N8b-1-Immunocytochemistry-Immunofluorescence-NBP1-47607-img0008.jpg)
![Immunohistochemistry: CACNB2 Antibody (N8b/1) [NBP1-47607] - Immunohistochemistry analysis using Mouse Anti-Cav Beta2 Calcium Channel Monoclonal Antibody, Clone S8b-1 (NBP1-47607). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Cav Beta2 Calcium Channel Monoclonal Antibody (NBP1-47607) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: All nuclei. Some nuclei also staining higher up in epidermis.](http://images.novusbio.com/fullsize/CACNB2-Antibody-N8b-1-Immunohistochemistry-NBP1-47607-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: CACNB2 Antibody (N8b/1) [NBP1-47607] - Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol at -20C for 10 minutes. Primary Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: All cells positive. Bright dottiness located throughout cytoplasm and in nuclei.. Image courtesy of Dr. Turksen, Ottawa Hospital Research Institute, Canada.](http://images.novusbio.com/fullsize/CACNB2-Antibody-N8b-1-Immunocytochemistry-Immunofluorescence-NBP1-47607-img0003.jpg)
![Western Blot: Vanilloid R-like 3/TRPV3 Antibody (N15/39) [NBP2-12918] - Western Blot analysis of Rat brain membrane lysate showing detection of TrpV3 protein using Mouse Anti-TrpV3 Monoclonal Antibody, Clone S15-39 (NBP2-12918). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-TrpV3 Monoclonal Antibody (NBP2-12918) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.](http://images.novusbio.com/fullsize/Vanilloid-R-like-3-TRPV3-Antibody-N15-39-Western-Blot-NBP2-12918-img0006.jpg "Western Blot: Vanilloid R-like 3/TRPV3 Antibody (N15/39) [NBP2-12918] - Western Blot analysis of Rat brain membrane lysate showing detection of TrpV3 protein using Mouse Anti-TrpV3 Monoclonal Antibody, Clone S15-39 (NBP2-12918). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-TrpV3 Monoclonal Antibody (NBP2-12918) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.")
![Immunocytochemistry/Immunofluorescence: Vanilloid R-like 3/TRPV3 Antibody (N15/39) [NBP2-12918] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-TrpV3 Monoclonal Antibody, Clone S15-39 (NBP2-12918). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-TrpV3 Monoclonal Antibody (NBP2-12918) at 1:50 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) TrpV3 Antibody (D) Composite.](http://images.novusbio.com/fullsize/Vanilloid-R-like-3-TRPV3-Antibody-N15-39-Immunocytochemistry-Immunofluorescence-NBP2-12918-img0008.jpg)
![Immunohistochemistry: Vanilloid R-like 3/TRPV3 Antibody (N15/39) [NBP2-12918] - Immunohistochemistry analysis using Mouse Anti-TrpV3 Monoclonal Antibody, Clone S15-39 (NBP2-12918). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-TrpV3 Monoclonal Antibody (NBP2-12918) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Filaggrin-like staining in upper layers. Dull lower layer cell staining. Some stain seen in hypodermis.](http://images.novusbio.com/fullsize/Vanilloid-R-like-3-TRPV3-Antibody-N15-39-Immunohistochemistry-NBP2-12918-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: Vanilloid R-like 3/TRPV3 Antibody (N15/39) [NBP2-12918] - Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20C. Primary Antibody: Mouse Anti-TrpV3 Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Dotty staining in all cells. Some intermediate filamentlike staining in some cells.](http://images.novusbio.com/fullsize/Vanilloid-R-like-3-TRPV3-Antibody-N15-39-Immunocytochemistry-Immunofluorescence-NBP2-12918-img0005.jpg)
![Western Blot: TRPC5 Antibody (N67/15) [NBP2-12919] - Western Blot analysis of Mouse brain showing detection of 110 kDa TrpC5 protein using Mouse Anti-TrpC5 Monoclonal Antibody, Clone N67/15 (NBP2-12919). Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse Brain. Load: 15 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Mouse Anti-TrpC5 Monoclonal Antibody (NBP2-12919) at 1:1000 for Overnight at 4C. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:7000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT. Predicted/Observed Size: 110 kDa.](http://images.novusbio.com/fullsize/TRPC5-Antibody-N67-15-Western-Blot-NBP2-12919-img0007.jpg "Western Blot: TRPC5 Antibody (N67/15) [NBP2-12919] - Western Blot analysis of Mouse brain showing detection of 110 kDa TrpC5 protein using Mouse Anti-TrpC5 Monoclonal Antibody, Clone N67/15 (NBP2-12919). Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse Brain. Load: 15 ug. Block: 5% Skim Milk powder in TBST. Primary Antibody: Mouse Anti-TrpC5 Monoclonal Antibody (NBP2-12919) at 1:1000 for Overnight at 4C. Secondary Antibody: Goat anti-mouse IgG:HRP at 1:7000 for 1 hour at RT with shaking. Color Development: Chemiluminescent for HRP (Moss) for 5 min in RT. Predicted/Observed Size: 110 kDa.")
![Immunocytochemistry/Immunofluorescence: TRPC5 Antibody (N67/15) [NBP2-12919] - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-TrpC5 Monoclonal Antibody, Clone N67/15 (NBP2-12919). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-TrpC5 Monoclonal Antibody (NBP2-12919) at 1:50 for overnight at 4C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) TrpC5 Antibody (D) Composite.](http://images.novusbio.com/fullsize/TRPC5-Antibody-N67-15-Immunocytochemistry-Immunofluorescence-NBP2-12919-img0009.jpg)
![Immunohistochemistry: TRPC5 Antibody (N67/15) [NBP2-12919] - Immunohistochemistry analysis using Mouse Anti-TrpC5 Monoclonal Antibody, Clone N67/15 (NBP2-12919). Tissue: Brain Slice. Species: Mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-TrpC5 Monoclonal Antibody (NBP2-12919) at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 l for 5 minutes at RT. Localization: Nuclear staining. Magnification: 10X.](http://images.novusbio.com/fullsize/TRPC5-Antibody-N67-15-Immunohistochemistry-NBP2-12919-img0008.jpg)
![Western Blot: TRPC5 Antibody (N67/15) [NBP2-12919] - Rat Brain Membrane showing detection of ~110 kDa TrpC5 protein using Mouse Anti-TrpC5 Monoclonal Antibody, Clone S67-15. Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Brain Membrane cell lysate. Load: 20 ug . Block: 2% BSA and 2% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-TrpC5 Monoclonal Antibody ) at 1:1000 for 16 hours at 4 degrees C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:100 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: ~110 kDa. Other Band(s): 160kDa, 60kDa.](http://images.novusbio.com/fullsize/TRPC5-Antibody-N67-15-Western-Blot-NBP2-12919-img0005.jpg)
![Western Blot: p38 alpha Antibody (9F12) [NB110-96875] - analysis of Human Cell lysates showing detection of p38 MAPK protein using Mouse Anti-p38 MAPK Monoclonal Antibody, Clone 9F12 . Load: 15 ug protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-p38 MAPK Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.](http://images.novusbio.com/fullsize/p38-alpha-Antibody-9F12-Western-Blot-NB110-96875-img0008.jpg "Western Blot: p38 alpha Antibody (9F12) [NB110-96875] - analysis of Human Cell lysates showing detection of p38 MAPK protein using Mouse Anti-p38 MAPK Monoclonal Antibody, Clone 9F12 . Load: 15 ug protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-p38 MAPK Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.")
![Immunohistochemistry: p38 alpha Antibody (9F12) [NB110-96875] - Immunohistochemistry analysis using Mouse Anti-p38 alpha Monoclonal Antibody, Clone 9F12 (NB110-96875). Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-p38 alpha Monoclonal Antibody (NB110-96875) at 1:10000 for 12 hours at 4C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 l for 2 minutes at RT. Magnification: 40x.](http://images.novusbio.com/fullsize/p38-alpha-Antibody-9F12-Immunohistochemistry-NB110-96875-img0009.jpg)
![Western Blot: AHSA1 Antibody (25F2.D10) - Azide and BSA Free [NBP2-12884] - Western Blot analysis of Human Cell lysates showing detection of AHSA1 protein using Rat Anti-Aha1 Monoclonal Antibody, Clone 25F2.D10 (NBP2-12884). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rat Anti-AHSA1 Monoclonal Antibody (NBP2-12884) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.](http://images.novusbio.com/fullsize/AHSA1-Antibody-25F2-D10-Azide-and-BSA-Free-Western-Blot-NBP2-12884-img0014.jpg "Western Blot: AHSA1 Antibody (25F2.D10) - Azide and BSA Free [NBP2-12884] - Western Blot analysis of Human Cell lysates showing detection of AHSA1 protein using Rat Anti-Aha1 Monoclonal Antibody, Clone 25F2.D10 (NBP2-12884). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rat Anti-AHSA1 Monoclonal Antibody (NBP2-12884) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.")
![Immunocytochemistry/Immunofluorescence: AHSA1 Antibody (25F2.D10) - Azide and BSA Free [NBP2-12884] - Tissue: HeLa cells. Species: Human. Primary Antibody: Rat Anti-Aha1 Monoclonal Antibody at 1:1000. Secondary Antibody: FITC Goat Anti-Rat (green).](http://images.novusbio.com/fullsize/AHSA1-Antibody-25F2-D10-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-12884-img0010.jpg)
![Immunohistochemistry: AHSA1 Antibody (25F2.D10) - Azide and BSA Free [NBP2-12884] - Immunohistochemistry analysis using Rat Anti-Aha1 Monoclonal Antibody, Clone 25F2.D10 (NBP2-12884). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Rat Anti-Aha1 Monoclonal Antibody (NBP2-12884) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Rat (green) at 1:50 for 1 hour at RT. Localization: Uppermost epidermis staining, and muscle.](http://images.novusbio.com/fullsize/AHSA1-Antibody-25F2-D10-Azide-and-BSA-Free-Immunohistochemistry-NBP2-12884-img0015.jpg)
![Immunoprecipitation: AHSA1 Antibody (25F2.D10) - Azide and BSA Free [NBP2-12884] - Immunoprecipitation analysis using Rat Anti-AHSA1 Monoclonal Antibody, Clone 25F2.D10. Tissue: HeLa cells. Species: Human. Primary Antibody: Rat Anti-AHSA1 Monoclonal Antibody at 1:1000.](http://images.novusbio.com/fullsize/AHSA1-Antibody-25F2-D10-Azide-and-BSA-Free-Immunoprecipitation-NBP2-12884-img0013.jpg)
![Flow Cytometry: Rat IgG Isotype Control [NBP1-71666] - Image from verified customer review.](http://images.novusbio.com/fullsize/Rat-IgG-Isotype-Control-Flow-Cytometry-NBP1-71666-img0001.jpg "Flow Cytometry: Rat IgG Isotype Control [NBP1-71666] - Image from verified customer review.")
![ELISA: Rat IgG Isotype Control [NBP1-71666] - ELISA plate was coated with serially diluted Rat IgG-UNLB. Immunoglobulin was detected with Goat Anti-Rat IgG-BIOT and Mouse Anti-Rat IgM-BIOT followed by Streptavidin-HRP and quantified.](http://images.novusbio.com/fullsize/Rat-IgG-Isotype-Control-ELISA-NBP1-71666-img0002.jpg)
![Western Blot: AHSA1 Antibody (25F2.D9) - Azide and BSA Free [NBP2-12883] - Western Blot analysis of Human Cell lysates showing detection of AHSA1 protein using Rat Anti-AHSA1 Monoclonal Antibody, Clone 25F2.D9 (NBP2-12883). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rat Anti-AHSA1 Monoclonal Antibody (NBP2-12883) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.](http://images.novusbio.com/fullsize/AHSA1-Antibody-25F2-D9-Azide-and-BSA-Free-Western-Blot-NBP2-12883-img0010.jpg "Western Blot: AHSA1 Antibody (25F2.D9) - Azide and BSA Free [NBP2-12883] - Western Blot analysis of Human Cell lysates showing detection of AHSA1 protein using Rat Anti-AHSA1 Monoclonal Antibody, Clone 25F2.D9 (NBP2-12883). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rat Anti-AHSA1 Monoclonal Antibody (NBP2-12883) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.")
![Immunocytochemistry/Immunofluorescence: AHSA1 Antibody (25F2.D9) - Azide and BSA Free [NBP2-12883] - Tissue: HeLa cells. Species: Human. Primary Antibody: Rat Anti-Aha1 Monoclonal Antibody at 1:1000. Secondary Antibody: FITC Goat Anti-Rat (green).](http://images.novusbio.com/fullsize/AHSA1-Antibody-25F2-D9-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-12883-img0007.jpg)
![Immunohistochemistry: AHSA1 Antibody (25F2.D9) - Azide and BSA Free [NBP2-12883] - Immunohistochemistry analysis using Rat Anti-Aha1 Monoclonal Antibody, Clone 25F2.D9 (NBP2-12883). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Rat Anti-Aha1 Monoclonal Antibody (NBP2-12883) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Rat (green) at 1:50 for 1 hour at RT. Localization: Uppermost epidermis staining, and muscle.](http://images.novusbio.com/fullsize/AHSA1-Antibody-25F2-D9-Azide-and-BSA-Free-Immunohistochemistry-NBP2-12883-img0011.jpg)
![Immunoprecipitation: AHSA1 Antibody (25F2.D9) - Azide and BSA Free [NBP2-12883] - Immunoprecipitation analysis using Rat Anti-AHSA1 Monoclonal Antibody, Clone 25F2.D9 . Tissue: HeLa cells. Species: Human. Primary Antibody: Rat Anti-AHSA1 Monoclonal Antibody at 1:1000.](http://images.novusbio.com/fullsize/AHSA1-Antibody-25F2-D9-Azide-and-BSA-Free-Immunoprecipitation-NBP2-12883-img0009.jpg)
![Western Blot: CDC7 Antibody (DCS-341) [NB120-10535] - Whole extract of A431 cells was separated on SDS-PAGE, blotted with Monoclonal Anti-Cdc7 Kinase Clone: DCS-341 and developed using Goat Anti-Mouse IgG-Peroxidase and a chemiluminescent substrate.](http://images.novusbio.com/fullsize/CDC7-Antibody-DCS-341-Western-Blot-NB120-10535-img0001.jpg "Western Blot: CDC7 Antibody (DCS-341) [NB120-10535] - Whole extract of A431 cells was separated on SDS-PAGE, blotted with Monoclonal Anti-Cdc7 Kinase Clone: DCS-341 and developed using Goat Anti-Mouse IgG-Peroxidase and a chemiluminescent substrate.")