Immunoprecipitation (IP) is a common research technique used to precipitate a specific protein (or protein complex) of interest out of a heterogeneous lysate. The concept of precipitating proteins out of solution is often called a "pull-down". There are a variety of methods to achieve immunoprecipitation, the most common of which uses antibodies bound to magnetic or agarose beads. This process can be used to isolate and concentrate a particular protein or complex from a sample containing many thousands of different proteins, and is often used to study protein-protein interactions through Co-IP experiments.
Novus offers a range of support options for IP when you buy a product to help you with your IP experiment including:
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56176SS | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Biotin | NBP2-21679 | |||
| DyLight 488 | NBP1-77702 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP1-27946SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF3129-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF3095-SP | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 350 | FAB918U-100UG | |||
| Alexa Fluor 405 | FAB918V-100UG | |||
| Alexa Fluor 488 | FAB918G-100UG | |||
| Alexa Fluor 532 | FAB918X-100UG | |||
| Alexa Fluor 594 | FAB918T-100UG | |||
| Alexa Fluor 647 | FAB918R-100UG | |||
| Alexa Fluor 700 | FAB918N-100UG | |||
| Alexa Fluor 750 | FAB918S-100UG | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB918-SP | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor Plus 488 | NB600-861AFP488 | |||
| Alexa Fluor Plus 555 | NB600-861AFP555 | |||
| Alexa Fluor Plus 594 | NB600-861AFP594 | |||
| Alexa Fluor Plus 647 | NB600-861AFP647 | |||
| Alexa Fluor Plus 680 | NB600-861AFP680 | |||
| Biotin | NB600-862-0.1mg | |||
| HRP | NB600-863-0.1mg | |||
![Western Blot: OCT1 Antibody [NBP2-21584] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with OCT1 antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/OCT1-Antibody-Western-Blot-NBP2-21584-img0011.jpg "Western Blot: OCT1 Antibody [NBP2-21584] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with OCT1 antibody diluted at 1:1000.")
![Immunohistochemistry-Paraffin: OCT1 Antibody [NBP2-21584] - Rat brain. OCT1 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/OCT1-Antibody-Immunohistochemistry-Paraffin-NBP2-21584-img0009.jpg)
![Immunohistochemistry-Paraffin: OCT1 Antibody [NBP2-21584] - Hela xenograft, using OCT1 antibody at 1:750 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min](http://images.novusbio.com/fullsize/OCT1-Antibody-Immunohistochemistry-Paraffin-NBP2-21584-img0002.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: OCT1 Antibody [NBP2-21584] - Wild-type (WT) and OCT1 knockout (KO) HeLa cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with OCT1 antibody diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-21584_rabbit-polyclonal-oct1-antibody-1610202419405718.jpg)
![Western Blot: NuMA Antibody [NB100-74636] - Detection of Human NUMA on HeLa whole cell lysate using NB100-74636. NUMA was also immunoprecipitated by rabbit anti-NUMA antibody NB100-74637.](http://images.novusbio.com/fullsize/NuMA-Antibody-Western-Blot-NB100-74636-img0008.jpg "<b>Independent Antibodies Validation. </b>Western Blot: NuMA Antibody [NB100-74636] - Detection of Human NUMA on HeLa whole cell lysate using NB100-74636. NUMA was also immunoprecipitated by rabbit anti-NUMA antibody NB100-74637.")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry: NuMA Antibody [NB100-74636] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- NUMA used at a dilution of 1:400 (0.5ug/ml). Detection: Red-fluorescent Goat anti-Rabbit IgG-heavy and light chain cross-adsorbed Antibody DyLight 594 Conjugated](http://images.novusbio.com/fullsize/NuMA-Antibody-Immunohistochemistry-NB100-74636-img0010.jpg)
![Immunohistochemistry-Paraffin: TRAF-3 Antibody - Unpurified [NB100-56176] - Analysis of formalin fixed paraffin-embedded (FFPE) human tonsil using this at 1:4000 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Staining was performed by Histowiz.](http://images.novusbio.com/fullsize/TRAF-3-Antibody-Immunohistochemistry-Paraffin-NB100-56176-img0001.jpg "Immunohistochemistry-Paraffin: TRAF-3 Antibody - Unpurified [NB100-56176] - Analysis of formalin fixed paraffin-embedded (FFPE) human tonsil using this at 1:4000 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Staining was performed by Histowiz.")
![Western Blot: Rel B Antibody [NBP2-20123] - Sample (30 ug of whole cell lysate) A: NIH-3T3 7. 5% SDS PAGE gel, diluted at 1:10000.](http://images.novusbio.com/fullsize/Rel-B-Antibody-Western-Blot-NBP2-20123-img0002.jpg "Western Blot: Rel B Antibody [NBP2-20123] - Sample (30 ug of whole cell lysate) A: NIH-3T3 7. 5% SDS PAGE gel, diluted at 1:10000.")
![Western Blot: Rabbit Polyclonal RelB Antibody [NBP2-20123] - Whole cell lysates from MDA-MB-231, SUM159, MCF-7 and HEK293T cells were loaded with 50 ug/lane. 10% SDS-PAGE. RRS1 Antibody (NBP2-49323) was used for primary antibody: 1:1000, 4C, overnight. Image from a verified customer review.](http://images.novusbio.com/fullsize/antibody/nbp2-20123_rabbit-polyclonal-relb-antibody-western-blot-311202415821..jpg)
![Western Blot: RelB Antibody [NBP2-20123] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with RelB antibody (NBP2-20123) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-20123_rabbit-polyclonal-relb-antibody-91020242075935.jpg)
![Western Blot: AKT1 [p Thr308] Antibody (18F3.H11) [NBP1-69924] - Lane 1: non-phosphorylated AKT in untreated cells. Lane 2: phosphorylated AKT on PDGF stimulated NIH/3T3 cell lysates. Load: 15 ug per lane. Primary antibody: AKT phospho Thr308 antibody at a 1:4000 dilution in TBS with 3% BSA, for 3 h at 4 C. Secondary antibody: peroxidase conjugated Gt-a-Mouse IgG (Fc) at a 1:40,000 dilution for 1 h at 4 C. Block: 3% BSA in TBS for 30 min at RT. Predicted/Observed size: (indicated by arrowhead at 56 kDa). Other band(s): unspecific.](http://images.novusbio.com/fullsize/AKT1-p-Thr308-Antibody-18F3-H11-Western-Blot-NBP1-69924-img0011.jpg "Western Blot: AKT1 [p Thr308] Antibody (18F3.H11) [NBP1-69924] - Lane 1: non-phosphorylated AKT in untreated cells. Lane 2: phosphorylated AKT on PDGF stimulated NIH/3T3 cell lysates. Load: 15 ug per lane. Primary antibody: AKT phospho Thr308 antibody at a 1:4000 dilution in TBS with 3% BSA, for 3 h at 4 C. Secondary antibody: peroxidase conjugated Gt-a-Mouse IgG (Fc) at a 1:40,000 dilution for 1 h at 4 C. Block: 3% BSA in TBS for 30 min at RT. Predicted/Observed size: (indicated by arrowhead at 56 kDa). Other band(s): unspecific.")
![Immunocytochemistry/Immunofluorescence: AKT1 [p Thr308] Antibody (18F3.H11) [NBP1-69924] - IHC-P analysis of FFPE human prostate tissue using AKT1 phospho Thr308 antibody at 20 ug/mL. Left panel: 20X magnification. Right panel: 40X magnification. Heat antigen retrieval in citrate buffer pH 6.2. Secondary antibody: streptavidin-HRP at 10 ug/mL. Antibody as precipitated red signal with hemotoxylin purple nuclear counterstain. Image using the Biotin format of this antibody.](http://images.novusbio.com/fullsize/AKT1-p-Thr308-Antibody-18F3-H11-Immunocytochemistry-Immunofluorescence-NBP1-69924-img0014.jpg)
![Immunohistochemistry-Paraffin: AKT1 [p Thr308] Antibody (18F3.H11) [NBP1-69924] - IHC analysis. Detection of phosphorylated AKT phospho Thr308 in human FFPE brain cerebellum tissue (40X). AKT1 phospho Thr308 antibody was used at 20 ug/mL. The image shows strong staining of Purkinje neurons. Red: AKT1 pT308. Purple: Hematoxylin nuclear counterstain.](http://images.novusbio.com/fullsize/AKT1-p-Thr308-Antibody-18F3-H11-Immunohistochemistry-Paraffin-NBP1-69924-img0003.jpg)
![Western Blot: AKT1 [p Thr308] Antibody (18F3.H11) [NBP1-69924] - WB analysis using the Biotin conjugate of AKT1 pT308 antibody. Detection of Lane 1: GST tagged AKT1 active recombinant protein. Lane 2: GST tagged AKT1 inactive recombinant protein. Load: 25 ng per lane. AKT pT308 Biotin conjugated antibody at 1:1000 overnight at 4C. Secondary antibody: HRP Streptavidin.](http://images.novusbio.com/fullsize/AKT1-p-Thr308-Antibody-18F3-H11-Western-Blot-NBP1-69924-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: AKT1 [p Thr308] Antibody (18F3.H11) [NBP1-69924] - ICC/IF analysis using the DyLight 488 conjugate of AKT1 pT308, NBP1-69924. Histone detection using DyLight 488 conjugate (green). Anti-Tubulin was detected using DyLight 549 conjugate (red). Nuclei were counter-stained using DAPI (blue).](http://images.novusbio.com/fullsize/AKT1-p-Thr308-Antibody-18F3-H11-Immunocytochemistry-Immunofluorescence-NBP1-69924-img0006.jpg)
![ELISA: AKT1 [p Thr308] Antibody (18F3.H11) [NBP1-69924] - ELISA of AKT1 phospho Thr308 Biotin conjugated antibody. Antigen: Unconjugated AKT1 phospho Thr308 and AKT1 non-phospho Thr308. Coating amount: 0.1 ug per well. Primary antibody: AKT1 phospho Thr308 Biotin conjugated antibody at 5 ug/mL. Dilution series: 3-fold. Mid-point concentration: 5 ng/mL AKT1 phospho Thr308 Biotin conjugated antibody. Secondary antibody: Peroxidase streptavidin secondary antibody at 1:10,000. Substrate: TMB](http://images.novusbio.com/fullsize/AKT1-p-Thr308-Antibody-18F3-H11-ELISA-NBP1-69924-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: FXR1 Antibody [NBP1-33719] - HeLa cells were fixed in ice-cold MeOH for 5 min.Green: FXR1 stained by FXR1 antibody diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [114] diluted at 1:1000.Blue: Fluoroshield with DAPI. Scale bar= 10um.](http://images.novusbio.com/fullsize/antibody/nbp1-33719_rabbit-polyclonal-fxr1-antibody-immunocytochemistry-immunofluorescence-8112023132213..jpg "Immunocytochemistry/Immunofluorescence: FXR1 Antibody [NBP1-33719] - HeLa cells were fixed in ice-cold MeOH for 5 min.Green: FXR1 stained by FXR1 antibody diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [114] diluted at 1:1000.Blue: Fluoroshield with DAPI. Scale bar= 10um.")
![Immunohistochemistry-Paraffin: FXR1 Antibody [NBP1-33719] - Analysis of paraffin-embedded sections of zebrafish kidney tube, using FXR1 antibody at 1:300 dilution.](http://images.novusbio.com/fullsize/antibody/nbp1-33719_rabbit-polyclonal-fxr1-antibody-immunohistochemistry-paraffin-8112023132323..jpg)
![Immunohistochemistry-Paraffin: FXR1 Antibody [NBP1-33719] - Mouse kidney.FXR1 stained by FXR1 antibody diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.](http://images.novusbio.com/fullsize/antibody/nbp1-33719_rabbit-polyclonal-fxr1-antibody-immunohistochemistry-paraffin-8112023132436..jpg)
![Western Blot: FXR1 Antibody [NBP1-33719] - Sample (30 ug of whole cell lysate) A: zebrafish brain 7.5% SDS PAGE NBP1-33719 diluted at 1:1000](http://images.novusbio.com/fullsize/nbp1-33719_rabbit-polyclonal-fxr1-antibody-910202420134079.jpg)
![Western Blot: THRAP3 Antibody [NB100-40848] - Detection of Human THRAP3/TRAP150 on HeLa whole cell lysate using NB100-40848. THRAP3/TRAP150 was also immunoprecipitated by other rabbit anti-THRAP3/TRAP150 antibodies and NB100-40847.](http://images.novusbio.com/fullsize/THRAP3-Antibody-Western-Blot-NB100-40848-img0002.jpg "<b>Independent Antibodies Validation. </b>Western Blot: THRAP3 Antibody [NB100-40848] - Detection of Human THRAP3/TRAP150 on HeLa whole cell lysate using NB100-40848. THRAP3/TRAP150 was also immunoprecipitated by other rabbit anti-THRAP3/TRAP150 antibodies and NB100-40847.")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: THRAP3 Antibody [NB100-40848] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti- THRAP3/TRAP150 used at a dilution of 1:1,000 (0.2ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/THRAP3-Antibody-Immunohistochemistry-Paraffin-NB100-40848-img0005.jpg)
![Western Blot: THRAP3 Antibody [NB100-40848] - ORF2p chA1-L1 antibody cross-reacts with BCLAF1. LNCaP cells were treated with Scramble, BCLAF1 (a) or THRAP3 (b) siRNA for 72 h. Whole cell lysates were assayed via western blot & probed with chA1-L1 ORF2p antibody, BCLAF1 or THRAP3, ORF1p, & Tubulin (loading control). Intensity of ORF1 bands in b were normalized to tubulin levels. c) Left: BCLAF1 Tagged overexpression construct with N-terminus 3x flag, V5, & Venus tag (~ 35 kDa). Right: HEK293 cells were transfected with vector only (VO) or tagged BCLAF1 construct. Whole cell lysates were assayed by western blot & probed with ORF2p antibody chA1-L1, & Tubulin (loading control) Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31890047), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb100-40848_rabbit-polyclonal-thrap3-antibody-310202415293318.jpg)
![Western Blot: Lactate Dehydrogenase Antibody [NB600-861] - Analysis using HRP conjugate of NB600-861. Lane 1-4: HeLa cell extracts cytoplasmic fraction (CF). Lane 5-8: HeLa cell extracts nuclear fraction (NF). Load: 30 ug per lane. Primary antibody: LDH antibody at 1:400 for overnight at 4C. Secondary antibody: IRDye800 secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO/TBST overnight at 4C. Predicted/Observed size: 36.6 kDa, 36 kDa for LDH. Other band(s): None.](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-Antibody-Western-Blot-NB600-861-img0012.jpg "Western Blot: Lactate Dehydrogenase Antibody [NB600-861] - Analysis using HRP conjugate of NB600-861. Lane 1-4: HeLa cell extracts cytoplasmic fraction (CF). Lane 5-8: HeLa cell extracts nuclear fraction (NF). Load: 30 ug per lane. Primary antibody: LDH antibody at 1:400 for overnight at 4C. Secondary antibody: IRDye800 secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO/TBST overnight at 4C. Predicted/Observed size: 36.6 kDa, 36 kDa for LDH. Other band(s): None.")
![Immunocytochemistry/Immunofluorescence: Lactate Dehydrogenase Antibody [NB600-861] - Analysis of HRP conjugate of NB600-861. Tissue: HeLa cells. Fixation: fixed for 5 min in 1:1 MeTOH:Acetone, blocked (preservative free) for 15 min. Antigen retrieval: not required. Primary antibody: Lactate Dehydrogenase antibody at 1:200 for](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-Antibody-Immunocytochemistry-Immunofluorescence-NB600-861-img0015.jpg)
![Western Blot: Lactate Dehydrogenase Antibody [NB600-861] - Analysis using Biotin conjugate of NB600-861. Lane 1: Lactate Dehydrogenase (Rabbit Muscle). Load: 50 ng per lane. Primary antibody: Goat anti-Lactate Dehydrogenase Antibody Biotin Conjugated at 1:1,000 overnight at 4C. Secondary antibody: HRP streptavidin secondary antibody at 1:40,000 for 30 min at RT. Block: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 36.5 kDa, 36.5 kDa for Lactate Dehydrogenase.](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-Antibody-Western-Blot-NB600-861-img0009.jpg)
![Western Blot: Lactate Dehydrogenase Antibody [NB600-861] - Analysis using Biotin conjugate of NB600-861. Lane 1-4: HeLa cell extracts cytoplasmic fraction (CF). Lane 5-8: HeLa cell extracts nuclear fraction (NF). Load: 30 ug per lane. Primary antibody: LDH antibody at 1:400 for overnight at 4C. Secondary antibody: IRDye800 secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO/TBST overnight at 4C. Predicted/Observed size: 36.6 kDa, 36 kDa for LDH. Other band(s): None.](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-Antibody-Western-Blot-NB600-861-img0010.jpg)
![Western Blot: Lactate Dehydrogenase Antibody [NB600-861] - Analysis using HRP conjugate of NB600-861. Lane 1: Lactate Dehydrogenase (Rabbit Muscle). Load: 50 ng per lane. Primary antibody: Goat anti-Lactate Dehydrogenase Antibody Peroxidase Conjugated at 1:1,000 overnight at 4C. Secondary antibody: n/a. Block: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 36.5 kDa, 36.5 kDa for Lactate Dehydrogenase.](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-Antibody-Western-Blot-NB600-861-img0011.jpg)
