Enzyme-linked immunosorbent assay (ELISA) is an analytic biochemical assay that utilizes enzyme-linked antibodies on a microwell platform to quantitate specific proteins within biological samples. Visual detection is then achieved by adding the enzyme’s substrate, which causes a color-changing reaction to occur. There are three different types of ELISA that are most commonly used: indirect ELISA, sandwich ELISA and competitive ELISA. These assays are extremely useful for high-throughput diagnostic screenings drug testing.
Novus offers a range of support options for ELISA when you buy a product to help you with your ELISA experiment including:
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-38483-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-92928-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-38493-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-93797-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-38010-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-92668-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-03216-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-38161-20ul | |||
![Immunocytochemistry/ Immunofluorescence: GPIP137 Antibody [NBP3-38483] - Immunofluorescence analysis of U-2 OS cells using GPIP137 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-38483_rabbit-gpip137-pab-1012202411343359.jpg "Immunocytochemistry/ Immunofluorescence: GPIP137 Antibody [NBP3-38483] - Immunofluorescence analysis of U-2 OS cells using GPIP137 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
![Western Blot: GPIP137 Antibody [NBP3-38483] - Western blot analysis of lysates from HeLa cells, using GPIP137 Rabbit pAb at 1:3000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp3-38483_rabbit-gpip137-pab-1012202411364637.jpg)
![Immunocytochemistry/ Immunofluorescence: GPIP137 Antibody [NBP3-38483] - Immunofluorescence analysis of L929 cells using GPIP137 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-38483_rabbit-gpip137-pab-101220241139291.jpg)
![Western Blot: GPIP137 Antibody [NBP3-38483] - Western blot analysis of lysates from Mouse brain, using GPIP137 Rabbit pAb at 1:3000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-38483_rabbit-gpip137-pab-1012202411404019.jpg)
![Immunocytochemistry/ Immunofluorescence: GPIP137 Antibody [NBP3-38483] - Immunofluorescence analysis of C6 cells using GPIP137 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-38483_rabbit-gpip137-pab-101220241140405.jpg)
![Simple Western: ATP5A Antibody [NBP2-92928] - ATP5A Antibodies (NBP2-92928), ProteinSimple Western Blot on Jess Instrument. One microgram of human brain tissue lysate was tested with the antibodies diluted 1:10 or 1:20 times. Image from verified customer review.](http://images.novusbio.com/fullsize/nbp2-92928_rabbit-polyclonal-atp5a-antibody-simple-western-2042023113534.jpg "Simple Western: ATP5A Antibody [NBP2-92928] - ATP5A Antibodies (NBP2-92928), ProteinSimple Western Blot on Jess Instrument. One microgram of human brain tissue lysate was tested with the antibodies diluted 1:10 or 1:20 times. Image from verified customer review.")
![Western Blot: ATP5A Antibody [NBP2-92928] - Analysis of extracts of various cell lines, using ATP5A at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 1s.](http://images.novusbio.com/fullsize/ATP5A-Antibody-Western-Blot-NBP2-92928-img0007.jpg)
![Immunocytochemistry/ Immunofluorescence: ATP5A Antibody - BSA Free [NBP2-92928] - Confocal immunofluorescence analysis of Hela cells using ATP5A Rabbit pAb at dilution of 1:200. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-92928_rabbit-polyclonal-atp5a-antibody-4220251430450.jpg)
![Immunocytochemistry/ Immunofluorescence: ATP5A Antibody - BSA Free [NBP2-92928] - Confocal immunofluorescence analysis of U-2OS cells using ATP5A Rabbit pAb at dilution of 1:200. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-92928_rabbit-polyclonal-atp5a-antibody-4220251429052.jpg)
![Western Blot: PERP Antibody (1O3L5) [NBP3-16617] - Western blot analysis of extracts of various cell lines, using PERP Rabbit mAb (NBP3-16617) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/PERP-Antibody-1O3L5-Western-Blot-NBP3-16617-img0006.jpg "Western Blot: PERP Antibody (1O3L5) [NBP3-16617] - Western blot analysis of extracts of various cell lines, using PERP Rabbit mAb (NBP3-16617) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunohistochemistry-Paraffin: PERP Antibody (1O3L5) [NBP3-16617] - Immunohistochemistry of paraffin-embedded rat skin using PERP Rabbit mAb (NBP3-16617) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/PERP-Antibody-1O3L5-Immunohistochemistry-Paraffin-NBP3-16617-img0001.jpg)
![Immunohistochemistry-Paraffin: PERP Antibody (1O3L5) [NBP3-16617] - Immunohistochemistry of paraffin-embedded mouse skin using PERP Rabbit mAb (NBP3-16617) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/PERP-Antibody-1O3L5-Immunohistochemistry-Paraffin-NBP3-16617-img0002.jpg)
![Immunohistochemistry: PERP Antibody (1O3L5) [NBP3-16617] - Immunofluorescence analysis of human skin cells using PERP Rabbit mAb (NBP3-16617) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/PERP-Antibody-1O3L5-Immunohistochemistry-NBP3-16617-img0003.jpg)
![Western Blot: ALK-1 Antibody (8L2V7) [NBP3-16876] - Analysis of extracts of various cell lines, using ALK-1 Rabbit mAb (NBP3-16876) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.](http://images.novusbio.com/fullsize/ALK-1-Antibody-8L2V7-Western-Blot-NBP3-16876-img0003.jpg "Western Blot: ALK-1 Antibody (8L2V7) [NBP3-16876] - Analysis of extracts of various cell lines, using ALK-1 Rabbit mAb (NBP3-16876) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.")
![Immunocytochemistry/Immunofluorescence: ALK-1 Antibody (8L2V7) [NBP3-16876] -Confocal imaging of C6 cells using ACVRL1 Rabbit mAb (dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). The cells were counterstained with alpha -Tubulin Mouse mAb (dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-16876_rabbit-alk-1-mab-8l2v7-immunocytochemistry-immunofluorescence-139202491229..jpg)
![Immunocytochemistry/Immunofluorescence: ALK-1 Antibody (8L2V7) [NBP3-16876] -Confocal imaging of NIH/3T3 cells using ACVRL1 Rabbit mAb (dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). The cells were counterstained with alpha -Tubulin Mouse mAb (dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-16876_rabbit-alk-1-mab-8l2v7-immunocytochemistry-immunofluorescence-139202491349..jpg)
![Immunocytochemistry/ Immunofluorescence: ALK-1 Antibody (8L2V7) [NBP3-16876] - Confocal imaging of C6 cells using ALK-1 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo(R) 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-16876_rabbit-alk-1-mab-8l2v7-52202511392299.jpg)
![Immunocytochemistry/ Immunofluorescence: ALK-1 Antibody (8L2V7) [NBP3-16876] - Confocal imaging of NIH/3T3 cells using ALK-1 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) . The cells were counterstained with alpha-Tubulin Mouse mAb followed by incubation with ABflo(R) 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-16876_rabbit-alk-1-mab-8l2v7-522025126539.jpg)
![Immunohistochemistry: MCCC2 Antibody [NBP3-38493] - Immunohistochemistry analysis of MCCC2 in paraffin-embedded rat kidney tissue using MCCC2 Rabbit pAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38493_rabbit-mccc2-pab-31120251744489.jpg%3Ft=1705995013 "Immunohistochemistry: MCCC2 Antibody [NBP3-38493] - Immunohistochemistry analysis of MCCC2 in paraffin-embedded rat kidney tissue using MCCC2 Rabbit pAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.")
![Western Blot: MCCC2 Antibody [NBP3-38493] - Western Blot analysis of various lysates using MCCC2 Rabbit pAb at 1:2000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Negative control (NC): MOLT-4.Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-38493_rabbit-mccc2-pab-311202517312.jpg%3Ft=1705995013)
![Immunohistochemistry: MCCC2 Antibody [NBP3-38493] - Immunohistochemistry analysis of MCCC2 in paraffin-embedded human liver tissue using MCCC2 Rabbit pAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38493_rabbit-mccc2-pab-311202517704.jpg%3Ft=1705995013)
![Immunohistochemistry: MCCC2 Antibody [NBP3-38493] - Immunohistochemistry analysis of MCCC2 in paraffin-embedded mouse kidney tissue using MCCC2 Rabbit pAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38493_rabbit-mccc2-pab-311202516593610.jpg%3Ft=1705995013)
![Immunohistochemistry: MCCC2 Antibody [NBP3-38493] - Immunohistochemistry analysis of MCCC2 in paraffin-embedded human colon tissue using MCCC2 Rabbit pAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38493_rabbit-mccc2-pab-3112025173155.jpg%3Ft=1705995013)
![Western Blot: UbcH8/Ube2L6 Antibody (k1H3) [NBP1-04351] - The Cell lysate (20ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human UBE2L6 antibody (1:2000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Jurkat cell lysate](http://images.novusbio.com/fullsize/UbcH8-Ube2L6-Antibody-k1H3-Western-Blot-NBP1-04351-img0006.jpg "Western Blot: UbcH8/Ube2L6 Antibody (k1H3) [NBP1-04351] - The Cell lysate (20ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human UBE2L6 antibody (1:2000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Jurkat cell lysate")
![Immunocytochemistry/Immunofluorescence: UbcH8/Ube2L6 Antibody (k1H3) [NBP1-04351] - ICC/IF analysis of UBE2L6 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human UBE2L6 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).](http://images.novusbio.com/fullsize/UbcH8-Ube2L6-Antibody-k1H3-Immunocytochemistry-Immunofluorescence-NBP1-04351-img0008.jpg)
![Immunohistochemistry-Paraffin: UbcH8/Ube2L6 Antibody (k1H3) [NBP1-04351] - Ube2L6 Antibody (k1H3) [NBP1-04351]-Paraffin embedded sections of human breast lobule tissue were incubated with anti-human UBE2L6 for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB)](http://images.novusbio.com/fullsize/UbcH8-Ube2L6-Antibody-k1H3-Immunohistochemistry-Paraffin-NBP1-04351-img0001.jpg)
![Western Blot: UbcH8/Ube2L6 Antibody (k1H3) [NBP1-04351] - Ube2L6 Antibody (k1H3) [NBP1-04351]-Cell lysates of MCF7 (20ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human UBE2L6 . Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.](http://images.novusbio.com/fullsize/UbcH8-Ube2L6-Antibody-k1H3-Western-Blot-NBP1-04351-img0002.jpg)
![Western Blot: UbcH8/Ube2L6 Antibody (k1H3) [NBP1-04351] - The Cell lysates (5ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human UBE2L6 antibody (1:2000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: 293T cell lysate Lane 2.: UBE2L6 Transfected 293T cell lysate](http://images.novusbio.com/fullsize/UbcH8-Ube2L6-Antibody-k1H3-Western-Blot-NBP1-04351-img0004.jpg)
![Western Blot: TAP2 Antibody - Azide and BSA Free [TAP2] - Western blot analysis of lysates from Rat spleen, using TAP2 Rabbit pAb at 1:500 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp2-93797_rabbit-polyclonal-tap2-antibody-5220251636348.jpg "Western Blot: TAP2 Antibody - Azide and BSA Free [TAP2] - Western blot analysis of lysates from Rat spleen, using TAP2 Rabbit pAb at 1:500 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.")
![Immunocytochemistry/Immunofluorescence: TAP2 Antibody [NBP2-93797] - Analysis of NIH/3T3 cells using TAP2 Rabbit pAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/TAP2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-93797-img0006.jpg)
![Immunohistochemistry-Paraffin: TAP2 Antibody [NBP2-93797] - Human colon carcinoma using TAP2 Rabbit pAb at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/TAP2-Antibody-Immunohistochemistry-Paraffin-NBP2-93797-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: TAP2 Antibody [NBP2-93797] - Analysis of C6 cells using TAP2 Rabbit pAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/TAP2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-93797-img0005.jpg)
![Immunohistochemistry: Elastin Antibody [NBP3-38010] - Immunohistochemistry analysis of paraffin-embedded Human esophagus tissue using Elastin Rabbit pAb at a dilution of 1:2000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38010_rabbit-elastin-pab-1012202411535876.jpg "Immunohistochemistry: Elastin Antibody [NBP3-38010] - Immunohistochemistry analysis of paraffin-embedded Human esophagus tissue using Elastin Rabbit pAb at a dilution of 1:2000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.")
![Western Blot: Elastin Antibody [NBP3-38010] - Western blot analysis of various lysates using Elastin Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.](http://images.novusbio.com/fullsize/nbp3-38010_rabbit-elastin-pab-1012202411592827.jpg)
![Immunohistochemistry: Elastin Antibody [NBP3-38010] - Immunohistochemistry analysis of Elastin in paraffin-embedded Human breast tissue using Elastin Rabbit pAb at a dilution of 1:2000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38010_rabbit-elastin-pab-3112025170470.jpg%3Ft=1705995530)
![Immunohistochemistry: Elastin Antibody [NBP3-38010] - Immunohistochemistry analysis of Elastin in paraffin-embedded Human esophagus tissue using Elastin Rabbit pAb at a dilution of 1:2000 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38010_rabbit-elastin-pab-3112025174447.jpg%3Ft=1705995530)
![Immunohistochemistry: RALBP1 Antibody (5C10W9) [NBP3-33554] - Immunohistochemistry analysis of RALBP1 in paraffin-embedded rat colon tissue using RALBP1 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-33554_rabbit-ralbp1-mab-5c10w9-31120251744411.jpg%3Ft=1705995169 "Immunohistochemistry: RALBP1 Antibody (5C10W9) [NBP3-33554] - Immunohistochemistry analysis of RALBP1 in paraffin-embedded rat colon tissue using RALBP1 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.")
![Immunohistochemistry: RALBP1 Antibody (5C10W9) [NBP3-33554] - Immunohistochemistry analysis of RALBP1 in paraffin-embedded mouse brain tissue using RALBP1 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-33554_rabbit-ralbp1-mab-5c10w9-31120251704725.jpg%3Ft=1705995169)
![Immunohistochemistry: RALBP1 Antibody (5C10W9) [NBP3-33554] - Immunohistochemistry analysis of RALBP1 in paraffin-embedded mouse testis tissue using RALBP1 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-33554_rabbit-ralbp1-mab-5c10w9-31120251704792.jpg%3Ft=1705995169)
![Immunohistochemistry: RALBP1 Antibody (5C10W9) [NBP3-33554] - Immunohistochemistry analysis of RALBP1 in paraffin-embedded human colon carcinoma tissue using RALBP1 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-33554_rabbit-ralbp1-mab-5c10w9-31120251744430.jpg%3Ft=1705995169)
![Immunocytochemistry/ Immunofluorescence: RALBP1 Antibody (5C10W9) [NBP3-33554] - Immunofluorescence analysis of U-2 OS cells using RALBP1 Rabbit mAb (A9701) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-33554_rabbit-ralbp1-mab-5c10w9-1012202411433436.jpg)
![Western Blot: 15-Lipoxygenase 2 Antibody [NBP2-92668] - Analysis of various lysates, using ALOX15B Rabbit pAb at 1:500 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp2-92668_rabbit-polyclonal-15-lipoxygenase-2-antibody-western-blot-2212025105133..png "Western Blot: 15-Lipoxygenase 2 Antibody [NBP2-92668] - Analysis of various lysates, using ALOX15B Rabbit pAb at 1:500 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.")
![Immunocytochemistry/ Immunofluorescence: 15-Lipoxygenase 2 Antibody - Azide and BSA Free [NBP2-92668] - Immunofluorescence analysis of NIH/3T3 cells using 15-Lipoxygenase 2 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-92668_rabbit-polyclonal-15-lipoxygenase-2-antibody-4220251425187.jpg)
![Immunocytochemistry/ Immunofluorescence: 15-Lipoxygenase 2 Antibody - Azide and BSA Free [NBP2-92668] - Immunofluorescence analysis of U2OS cells using 15-Lipoxygenase 2 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-92668_rabbit-polyclonal-15-lipoxygenase-2-antibody-42202514264439.jpg)
![Immunocytochemistry/ Immunofluorescence: 15-Lipoxygenase 2 Antibody - Azide and BSA Free [NBP2-92668] - Immunofluorescence analysis of PC-12 cells using 15-Lipoxygenase 2 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-92668_rabbit-polyclonal-15-lipoxygenase-2-antibody-42202514275258.jpg)
![Western Blot: UBE2O Antibody [NBP3-03216] - Analysis of extracts from normal (control) and UBE2O knockout (KO) HeLa cells, using UBE2O antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.](http://images.novusbio.com/fullsize/UBE2O-Antibody-Western-Blot-NBP3-03216-img0003.jpg "<b>Genetic Strategies Validation. </b>Western Blot: UBE2O Antibody [NBP3-03216] - Analysis of extracts from normal (control) and UBE2O knockout (KO) HeLa cells, using UBE2O antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.")
![<b>Genetic Strategies Validation. </b>Western Blot: UBE2O Antibody [NBP3-03216] - Analysis of 200ug extracts of A-431 cells using UBE2O antibody . Western blot was performed from the immunoprecipitate using this antibody.](http://images.novusbio.com/fullsize/UBE2O-Antibody-Western-Blot-NBP3-03216-img0002.jpg)
![Western Blot: UBE2O Antibody [NBP3-03216] - Analysis of extracts of various cell lines, using UBE2O antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit](http://images.novusbio.com/fullsize/UBE2O-Antibody-Western-Blot-NBP3-03216-img0004.jpg)
![Immunocytochemistry/ Immunofluorescence: UBE2O Antibody - BSA Free [NBP3-03216] - Immunofluorescence analysis of HeLa cells using [KO Validated] UBE2O Rabbit pAb (A10036) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-03216_rabbit-polyclonal-ube2o-antibody-52202519302932.jpg)
![Immunoprecipitation: Proteasome 20S beta2 Antibody [NBP3-38161] - Immunoprecipitation analysis of 300 ug extracts of HeLa cells using 3 ug Proteasome 20S beta2 antibody. Western blot was performed from the immunoprecipitate using Proteasome 20S beta2 antibody at a dilution of 1:1000.](http://images.novusbio.com/fullsize/nbp3-38161_rabbit-proteasome-20s-beta2-pab-91220241517113.jpg "Immunoprecipitation: Proteasome 20S beta2 Antibody [NBP3-38161] - Immunoprecipitation analysis of 300 ug extracts of HeLa cells using 3 ug Proteasome 20S beta2 antibody. Western blot was performed from the immunoprecipitate using Proteasome 20S beta2 antibody at a dilution of 1:1000.")
![Western Blot: Proteasome 20S beta2 Antibody [NBP3-38161] - Western blot analysis of various lysates using Proteasome 20S beta2 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp3-38161_rabbit-proteasome-20s-beta2-pab-912202415171118.jpg)
![Western Blot: Proteasome 20S beta2 Antibody [NBP3-38161] - Western blot analysis of various lysates using Proteasome 20S beta2 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.](http://images.novusbio.com/fullsize/nbp3-38161_rabbit-proteasome-20s-beta2-pab-91220241519536.jpg)
![Immunohistochemistry: Proteasome 20S beta2 Antibody [NBP3-38161] - Immunohistochemistry analysis of paraffin-embedded Human esophageal cancer using Proteasome 20S beta2 Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.](http://images.novusbio.com/fullsize/nbp3-38161_rabbit-proteasome-20s-beta2-pab-912202414112848.jpg)