Enzyme-linked immunosorbent assay (ELISA) is an analytic biochemical assay that utilizes enzyme-linked antibodies on a microwell platform to quantitate specific proteins within biological samples. Visual detection is then achieved by adding the enzyme’s substrate, which causes a color-changing reaction to occur. There are three different types of ELISA that are most commonly used: indirect ELISA, sandwich ELISA and competitive ELISA. These assays are extremely useful for high-throughput diagnostic screenings drug testing.
Novus offers a range of support options for ELISA when you buy a product to help you with your ELISA experiment including:
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | FAB1642P | |||
| Carrier Free | MAB1642-SP | |||
| Carrier Free | FAB1642V-100UG | |||
| Carrier Free | FAB1642U-100UG | |||
| Carrier Free | FAB1642T-100UG | |||
| Carrier Free | FAB1642S-100UG | |||
| Carrier Free | FAB1642R-100UG | |||
| Carrier Free | FAB1642N-100UG | |||
| Carrier Free | FAB1642G-100UG | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB2281-SP | |||
| Carrier Free | MAB2281-500 | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor Plus 488 | NB600-861AFP488 | |||
| Alexa Fluor Plus 555 | NB600-861AFP555 | |||
| Alexa Fluor Plus 594 | NB600-861AFP594 | |||
| Alexa Fluor Plus 647 | NB600-861AFP647 | |||
| Alexa Fluor Plus 680 | NB600-861AFP680 | |||
| Biotin | NB600-862-0.1mg | |||
| HRP | NB600-863-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-54473-100ug | |||
![N/A Nesfatin-1/Nucleobindin-2 [Biotin]](http://images.novusbio.com/fullsize2/DATA_Nesfatin1_DY5949_ELISA_2145.jpg "N/A Nesfatin-1/Nucleobindin-2 [Biotin]")
![N/A CXCL6/GCP-2 [Biotin]](http://images.novusbio.com/fullsize2/DATA_CXCL6_DY333_ELISA_1935.jpg "N/A CXCL6/GCP-2 [Biotin]")
![N/A EMMPRIN/CD147 [Biotin]](http://images.novusbio.com/fullsize2/DATA_EMMPRIN_DY972_ELISA_2286.jpg "N/A EMMPRIN/CD147 [Biotin]")
![N/A IL-28B/IFN-lambda 3 [HRP]](http://images.novusbio.com/fullsize2/IL28B_D28B00_ELISA_1128.jpg "N/A IL-28B/IFN-lambda 3 [HRP]")
![N/A IL-28B/IFN-lambda 3 [HRP]](http://images.novusbio.com/fullsize2/IL28B_D28B00_ELISA_1127.jpg)
![Western Blot: Lactate Dehydrogenase Antibody [NB600-861] - Analysis using HRP conjugate of NB600-861. Lane 1-4: HeLa cell extracts cytoplasmic fraction (CF). Lane 5-8: HeLa cell extracts nuclear fraction (NF). Load: 30 ug per lane. Primary antibody: LDH antibody at 1:400 for overnight at 4C. Secondary antibody: IRDye800 secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO/TBST overnight at 4C. Predicted/Observed size: 36.6 kDa, 36 kDa for LDH. Other band(s): None.](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-Antibody-Western-Blot-NB600-861-img0012.jpg "Western Blot: Lactate Dehydrogenase Antibody [NB600-861] - Analysis using HRP conjugate of NB600-861. Lane 1-4: HeLa cell extracts cytoplasmic fraction (CF). Lane 5-8: HeLa cell extracts nuclear fraction (NF). Load: 30 ug per lane. Primary antibody: LDH antibody at 1:400 for overnight at 4C. Secondary antibody: IRDye800 secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO/TBST overnight at 4C. Predicted/Observed size: 36.6 kDa, 36 kDa for LDH. Other band(s): None.")
![Immunocytochemistry/Immunofluorescence: Lactate Dehydrogenase Antibody [NB600-861] - Analysis of HRP conjugate of NB600-861. Tissue: HeLa cells. Fixation: fixed for 5 min in 1:1 MeTOH:Acetone, blocked (preservative free) for 15 min. Antigen retrieval: not required. Primary antibody: Lactate Dehydrogenase antibody at 1:200 for](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-Antibody-Immunocytochemistry-Immunofluorescence-NB600-861-img0015.jpg)
![Western Blot: Lactate Dehydrogenase Antibody [NB600-861] - Analysis using Biotin conjugate of NB600-861. Lane 1: Lactate Dehydrogenase (Rabbit Muscle). Load: 50 ng per lane. Primary antibody: Goat anti-Lactate Dehydrogenase Antibody Biotin Conjugated at 1:1,000 overnight at 4C. Secondary antibody: HRP streptavidin secondary antibody at 1:40,000 for 30 min at RT. Block: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 36.5 kDa, 36.5 kDa for Lactate Dehydrogenase.](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-Antibody-Western-Blot-NB600-861-img0009.jpg)
![Western Blot: Lactate Dehydrogenase Antibody [NB600-861] - Analysis using Biotin conjugate of NB600-861. Lane 1-4: HeLa cell extracts cytoplasmic fraction (CF). Lane 5-8: HeLa cell extracts nuclear fraction (NF). Load: 30 ug per lane. Primary antibody: LDH antibody at 1:400 for overnight at 4C. Secondary antibody: IRDye800 secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO/TBST overnight at 4C. Predicted/Observed size: 36.6 kDa, 36 kDa for LDH. Other band(s): None.](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-Antibody-Western-Blot-NB600-861-img0010.jpg)
![Western Blot: Lactate Dehydrogenase Antibody [NB600-861] - Analysis using HRP conjugate of NB600-861. Lane 1: Lactate Dehydrogenase (Rabbit Muscle). Load: 50 ng per lane. Primary antibody: Goat anti-Lactate Dehydrogenase Antibody Peroxidase Conjugated at 1:1,000 overnight at 4C. Secondary antibody: n/a. Block: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 36.5 kDa, 36.5 kDa for Lactate Dehydrogenase.](http://images.novusbio.com/fullsize/Lactate-Dehydrogenase-Antibody-Western-Blot-NB600-861-img0011.jpg)
![N/A EGFR [HRP]](http://images.novusbio.com/fullsize2/DATA_EGF_R_DEGFR0_ELISA_655.jpg "N/A EGFR [HRP]")
![N/A EGFR [HRP]](http://images.novusbio.com/fullsize2/DATA_EGF_R_DEGFR0_ELISA_656.jpg)
![N/A EGFR [HRP]](http://images.novusbio.com/fullsize2/EGF_R_DEGFR0_ELISA_80.jpg)
![N/A C-Peptide [HRP]](http://images.novusbio.com/fullsize2/DATA_Insulin_DICP00_ELISA_720.jpg "N/A C-Peptide [HRP]")
![N/A C-Peptide [HRP]](http://images.novusbio.com/fullsize2/Insulin_DICP00_ELISA_478.jpg)
![N/A IGF-II/IGF2 [Biotin]](http://images.novusbio.com/fullsize2/DATA_IGFII_DY29205_ELISA_2416.jpg "N/A IGF-II/IGF2 [Biotin]")
![Western Blot: Siglec-2/CD22 Antibody (BLCAM/1795) [NBP2-53239] - Western Blot Analysis of Raji cell lysate using Siglec-2/CD22 Antibody (BLCAM/1795).](http://images.novusbio.com/fullsize/Siglec-2-CD22-Antibody-BLCAM-1795-Western-Blot-NBP2-53239-img0008.jpg "Western Blot: Siglec-2/CD22 Antibody (BLCAM/1795) [NBP2-53239] - Western Blot Analysis of Raji cell lysate using Siglec-2/CD22 Antibody (BLCAM/1795).")
![Immunocytochemistry/Immunofluorescence: Siglec-2/CD22 Antibody (BLCAM/1795) [NBP2-53239] - Immunofluorescent staining of paraformaldehyde-fixed Ramos cells using Siglec-2/CD22 Antibody (BLCAM/1795) followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are stained with Red Dot.](http://images.novusbio.com/fullsize/Siglec-2-CD22-Antibody-BLCAM-1795-Immunocytochemistry-Immunofluorescence-NBP2-53239-img0005.jpg)
![Immunohistochemistry-Paraffin: Siglec-2/CD22 Antibody (BLCAM/1795) [NBP2-53239] - Formalin-fixed, paraffin-embedded human Spleen stained with Siglec-2/CD22 Antibody (BLCAM/1795).](http://images.novusbio.com/fullsize/Siglec-2-CD22-Antibody-BLCAM-1795-Immunohistochemistry-Paraffin-NBP2-53239-img0006.jpg)
![Flow Cytometry: Siglec-2/CD22 Antibody (BLCAM/1795) [NBP2-53239] - Flow Cytometric Analysis of paraformaldehyde-fixed MOLT4 cells. Siglec-2/CD22 Antibody (BLCAM/1795) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/Siglec-2-CD22-Antibody-BLCAM-1795-Flow-Cytometry-NBP2-53239-img0004.jpg)
![Western Blot: Siglec-2/CD22 Antibody (BLCAM/1795) [NBP2-53239] - Analysis of Raji and Ramos Cell Lysates using CD22 Monoclonal Antibody (BLCAM/1795).](http://images.novusbio.com/fullsize/Siglec-2-CD22-Antibody-BLCAM-1795-Western-Blot-NBP2-53239-img0001.jpg)
![Immunohistochemistry-Paraffin: Siglec-2/CD22 Antibody (BLCAM/1795) [NBP2-53239] - Formalin-fixed, paraffin-embedded human Tonsil stained with CD22 Monoclonal Antibody (BLCAM/1795).](http://images.novusbio.com/fullsize/Siglec-2-CD22-Antibody-BLCAM-1795-Immunohistochemistry-Paraffin-NBP2-53239-img0002.jpg)
![Protein Array: Siglec-2/CD22 Antibody (BLCAM/1795) [NBP2-53239] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Siglec-2/CD22 Antibody (BLCAM/1795) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.](http://images.novusbio.com/fullsize/Siglec-2-CD22-Antibody-BLCAM-1795-Protein-Array-NBP2-53239-img0007.jpg)